FastQCFastQC Report
Thu 26 May 2016
SRR1546693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546693_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1075274
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46890.4360748981189911No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37640.3500503127574925No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11160.10378749974425124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11120.1034155015372826No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGT207.0330827E-445.00000440
ACTAGCA207.0330827E-445.00000414
ACTACGG207.0330827E-445.0000042
TATAGTC207.0330827E-445.00000440
ATACCCG207.0330827E-445.00000411
TCGAAAC207.0330827E-445.00000430
GTTGACG351.211829E-745.01
CTATACG253.890637E-545.01
ACGGGTA1350.045.05
CATAGTT253.890637E-545.036
GTATGCG551.8189894E-1245.01
TACGACG253.890637E-545.03
CGAATCG253.890637E-545.02
CTCGATG351.211829E-745.01
ACGTAGC253.890637E-545.019
ATACGAG502.1827873E-1145.01
ATACGAC253.890637E-545.02
AACACGT551.8189894E-1245.041
CACGACA302.1653705E-644.99999616
CGAATAT1150.044.99999614