##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546693_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1075274
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33897034616293	31.0	31.0	33.0	30.0	34.0
2	31.731696293223866	31.0	31.0	34.0	30.0	34.0
3	31.843440834615176	31.0	31.0	34.0	30.0	34.0
4	35.60362940050629	37.0	35.0	37.0	33.0	37.0
5	35.50576411221698	37.0	35.0	37.0	33.0	37.0
6	35.56603433171452	37.0	35.0	37.0	33.0	37.0
7	35.93705511339435	37.0	35.0	37.0	35.0	37.0
8	35.91760611713852	37.0	35.0	37.0	35.0	37.0
9	37.642292104152055	39.0	37.0	39.0	35.0	39.0
10	37.13528830791036	39.0	37.0	39.0	33.0	39.0
11	36.819000552417336	39.0	35.0	39.0	32.0	39.0
12	35.854991378941556	37.0	35.0	39.0	32.0	39.0
13	35.475466718250416	37.0	35.0	39.0	31.0	39.0
14	36.394828666925825	38.0	35.0	41.0	31.0	41.0
15	36.7040140466523	38.0	35.0	41.0	32.0	41.0
16	36.86010542429185	38.0	35.0	41.0	32.0	41.0
17	36.80877711169432	38.0	35.0	41.0	32.0	41.0
18	36.72476224664597	38.0	35.0	40.0	32.0	41.0
19	36.63695113989551	37.0	35.0	40.0	32.0	41.0
20	36.46616025310758	37.0	35.0	40.0	31.0	41.0
21	36.34704735723174	37.0	35.0	40.0	31.0	41.0
22	36.208438965324184	37.0	35.0	40.0	31.0	41.0
23	36.153909608155686	37.0	35.0	40.0	31.0	41.0
24	36.01234476049825	36.0	35.0	40.0	31.0	41.0
25	35.9146375714469	36.0	35.0	40.0	31.0	41.0
26	35.7540171156375	36.0	34.0	40.0	30.0	41.0
27	35.65511953232385	36.0	34.0	40.0	30.0	41.0
28	35.59134880969874	36.0	34.0	40.0	30.0	41.0
29	35.66674540628714	36.0	35.0	40.0	30.0	41.0
30	35.630261682138695	36.0	35.0	40.0	30.0	41.0
31	35.49487572469901	36.0	34.0	40.0	30.0	41.0
32	35.28059545752989	36.0	34.0	40.0	29.0	41.0
33	35.119513723943854	36.0	34.0	40.0	29.0	41.0
34	34.95331980499854	36.0	34.0	40.0	27.0	41.0
35	34.81190561661493	35.0	34.0	40.0	27.0	41.0
36	34.616853936764024	35.0	34.0	40.0	25.0	41.0
37	34.54258728472929	35.0	34.0	40.0	25.0	41.0
38	34.487771488941426	35.0	33.0	40.0	26.0	41.0
39	34.4464369081741	35.0	34.0	40.0	25.0	41.0
40	34.25989561730312	35.0	33.0	40.0	24.0	41.0
41	34.240540550594545	35.0	34.0	40.0	24.0	41.0
42	34.14353550815885	35.0	33.0	40.0	24.0	41.0
43	33.98577199857897	35.0	33.0	40.0	23.0	41.0
44	33.795400986167245	35.0	33.0	40.0	23.0	41.0
45	33.65455316505374	35.0	33.0	39.0	23.0	41.0
46	33.61306420503053	35.0	33.0	39.0	23.0	41.0
47	33.589210750004185	35.0	33.0	39.0	23.0	41.0
48	33.524016204241896	35.0	33.0	39.0	23.0	41.0
49	33.47196063514974	35.0	33.0	39.0	23.0	41.0
50	33.320548994953846	35.0	33.0	39.0	23.0	41.0
51	32.9481341499934	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	0.0
12	2.0
13	6.0
14	8.0
15	31.0
16	95.0
17	281.0
18	528.0
19	1091.0
20	1973.0
21	3135.0
22	4665.0
23	6718.0
24	10070.0
25	13819.0
26	18326.0
27	21303.0
28	23410.0
29	26155.0
30	30719.0
31	36382.0
32	44695.0
33	58407.0
34	106279.0
35	184495.0
36	77087.0
37	89750.0
38	122601.0
39	193080.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.11818755033601	23.147774427727256	28.483995707140693	14.250042314796044
2	29.97840550408547	26.770757964946608	29.935625710284075	13.315210820683845
3	27.846483779948176	25.62509648703493	32.4373136521482	14.09110608086869
4	24.10390281918841	29.24240705159801	31.3606578416292	15.293032287584374
5	23.092718693095897	32.79405993263113	29.937206702663694	14.176014671609282
6	21.06458446870286	41.05586111074945	26.635815615368735	11.243738805178959
7	85.81580136783741	5.192815970627022	7.118278689896715	1.873103971638857
8	86.32385791900484	4.244964539270921	6.667602862154204	2.7635746795700444
9	81.13178594479174	5.7212394236259785	9.637729546143587	3.5092450854386885
10	54.54200510753539	19.049005183795014	13.805690456572	12.603299252097605
11	47.07116511698414	18.890719946729856	19.499588011985782	14.538526924300225
12	40.635131138667916	19.18534252664902	24.588244484661583	15.591281850021483
13	22.13770629625565	37.62808363263689	25.848295411216114	14.385914659891338
14	16.209449870451625	40.37054741396147	28.627308016375363	14.79269469921155
15	14.213028493202662	20.45348441420512	49.950431238921425	15.383055853670784
16	16.14946515957793	16.721412402792218	47.27102115367804	19.85810128395181
17	17.023846945057723	17.347764383775672	29.118066650918745	36.51032202024786
18	22.266603674970288	21.38831590831732	35.02790916547782	21.31717125123457
19	29.67717995599261	23.570364390843636	25.98128477020741	20.771170882956344
20	31.505737142346973	21.54846113641732	24.84882922864312	22.096972492592588
21	23.78435635940235	27.542561244854802	27.493271482431453	21.179810913311396
22	24.058054040179528	23.3009446894466	24.84464424881472	27.796357021559153
23	20.223775521401986	29.132760580094004	23.82499716351367	26.818466734990338
24	21.508006331409483	22.165048164467844	37.24873846108061	19.078207043042053
25	18.472593962097104	23.343910482351475	34.664094919062485	23.519400636488932
26	17.273643741037166	31.491973208689135	28.319200501453583	22.915182548820116
27	18.739409676045362	31.8624834228299	28.007373004462117	21.390733896662617
28	16.871420679752326	27.44825969938825	37.10830913794996	18.572010482909473
29	17.498981654908423	23.541441530251824	35.70736389050605	23.252212924333705
30	20.688029283698853	28.315480519383897	31.541541969767707	19.454948227149547
31	30.120136820940523	25.47648320335096	24.241263157111582	20.162116818596935
32	31.460446360648543	24.83608829005444	25.609751560997474	18.09371378829954
33	29.927162751075542	25.607705570859146	24.451814142255834	20.013317535809477
34	22.392153069822204	25.346004832256707	28.139153369280763	24.12268872864033
35	22.126453350494852	25.01204344195061	31.161545801349238	21.699957406205304
36	31.344010921867355	23.245795955263496	27.24282368958982	18.167369433279333
37	22.855662835705132	30.123670803906727	28.354447331563865	18.666219028824283
38	22.066282640517674	31.365586817871538	24.82725333263894	21.740877208971852
39	22.04796172882447	29.73697866776282	27.037387679791387	21.17767192362133
40	25.341261855117857	24.20499333193214	26.55574300131873	23.898001811631268
41	18.75698659132463	23.133359497207223	27.310806361913336	30.798847549554807
42	24.010345270135797	23.692193803625866	24.106227807981963	28.19123311825637
43	23.69191480497064	23.342515489075343	26.472322403406018	26.493247302548
44	21.603330871945197	28.207880038018217	28.230757927746787	21.9580311622898
45	18.550620586008776	34.690506791757265	24.008950276859668	22.749922345374294
46	23.142845451484924	29.989658449846274	26.887658401486505	19.979837697182298
47	22.813720037869416	26.20336769976769	27.183954973337027	23.798957289025868
48	24.53979171820392	22.51984145436419	30.18244652060777	22.75792030682412
49	23.06565582353893	20.937640080574813	32.12920613722642	23.86749795865984
50	20.587403768713834	30.520313892087042	27.39273896699818	21.499543372200947
51	20.219218543366622	31.176797727834956	24.63623225336054	23.967751475437886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1570.0
1	1781.5
2	1993.0
3	5465.5
4	8938.0
5	6627.5
6	4317.0
7	4654.5
8	4992.0
9	5567.5
10	6143.0
11	6562.5
12	6982.0
13	6810.5
14	6639.0
15	6201.0
16	5763.0
17	5760.5
18	5758.0
19	5474.5
20	5191.0
21	5389.5
22	5588.0
23	5079.5
24	4571.0
25	4766.0
26	5193.0
27	5425.0
28	6307.5
29	7190.0
30	8075.0
31	8960.0
32	11157.5
33	13355.0
34	16686.5
35	20018.0
36	20894.0
37	21770.0
38	25825.5
39	29881.0
40	44712.0
41	59543.0
42	75851.5
43	92160.0
44	96703.0
45	101246.0
46	100350.0
47	99454.0
48	95795.0
49	92136.0
50	88366.5
51	84597.0
52	76444.0
53	68291.0
54	61127.0
55	53963.0
56	51502.0
57	49041.0
58	46609.0
59	44177.0
60	41240.5
61	38304.0
62	35265.5
63	32227.0
64	29727.0
65	27227.0
66	22828.5
67	18430.0
68	15148.0
69	11866.0
70	10374.0
71	8882.0
72	7337.0
73	5792.0
74	4811.5
75	2774.0
76	1717.0
77	1369.0
78	1021.0
79	861.5
80	702.0
81	565.0
82	428.0
83	263.0
84	98.0
85	90.0
86	82.0
87	56.5
88	31.0
89	21.5
90	12.0
91	9.5
92	7.0
93	4.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1075274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.109506923745478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.97821167436193	19.52344755035556
2	6.550439986917265	3.1585575643632207
3	2.2318208692581396	1.6142430209981629
4	1.123845771961392	1.0838146968130105
5	0.7566603031392584	0.9121353408729651
6	0.5846538936571983	0.8457430258273786
7	0.45482222735160427	0.767587774758479
8	0.3869296132465504	0.7462945751655893
9	0.3249917526420586	0.7051851819435501
>10	4.625647160845189	27.464207473227038
>50	1.4314923072921297	24.188170000464655
>100	0.5473809329744697	17.776313676993006
>500	0.001551753176398213	0.2153060827340729
>1k	0.001551753176398213	0.998994035483333
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4689	0.4360748981189911	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3764	0.3500503127574925	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1116	0.10378749974425124	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1112	0.1034155015372826	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.056822726114460126	0.0
2	0.0	0.0	0.0	0.24365882556446078	0.0
3	0.0	0.0	0.0	0.34447033965296286	0.0
4	0.0	0.0	0.0	0.5431173821742179	0.0
5	0.0	0.0	0.0	0.8359729706102816	0.0
6	0.0	0.0	0.0	1.38513532364774	0.0
7	0.0	0.0	0.0	1.6657149712538386	0.0
8	0.0	0.0	0.0	2.556743676495479	0.0
9	0.0	0.0	0.0	3.087120120081021	0.0
10	0.0	0.0	0.0	3.9478309714547177	0.0
11	0.0	0.0	0.0	4.591108870855242	0.0
12	0.0	0.0	0.0	5.135993244512561	0.0
13	0.0	0.0	0.0	5.399739973253329	0.0
14	0.0	0.0	0.0	5.509665443412563	0.0
15	0.0	0.0	0.0	5.624984887572842	0.0
16	0.0	0.0	0.0	5.85646077185908	0.0
17	0.0	0.0	0.0	6.089889646731903	0.0
18	0.0	0.0	0.0	6.466444831735911	0.0
19	0.0	0.0	0.0	6.616546108247758	0.0
20	0.0	0.0	0.0	6.78059731752093	0.0
21	0.0	0.0	0.0	6.979616358249153	0.0
22	0.0	0.0	0.0	7.161430481905077	0.0
23	0.0	0.0	0.0	7.375515450015531	0.0
24	0.0	0.0	0.0	7.540031657047413	0.0
25	0.0	0.0	0.0	7.67246301872825	0.0
26	0.0	0.0	0.0	7.8048943804090865	0.0
27	0.0	0.0	0.0	7.938813734917797	0.0
28	9.299955174216061E-5	0.0	0.0	8.071338096150377	0.0
29	9.299955174216061E-5	0.0	0.0	8.202560463658566	0.0
30	9.299955174216061E-5	0.0	0.0	8.408182472560481	0.0
31	9.299955174216061E-5	0.0	0.0	8.57418667242024	0.0
32	9.299955174216061E-5	0.0	0.0	8.70550203948017	0.0
33	9.299955174216061E-5	0.0	0.0	8.855789315095501	0.0
34	9.299955174216061E-5	0.0	0.0	8.999194623881912	0.0
35	9.299955174216061E-5	0.0	0.0	9.185100727814492	0.0
36	9.299955174216061E-5	0.0	0.0	9.34385096263836	0.0
37	9.299955174216061E-5	0.0	0.0	9.49627722794376	0.0
38	9.299955174216061E-5	0.0	0.0	9.668326398666759	0.0
39	9.299955174216061E-5	0.0	0.0	9.841584563562403	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTGT	20	7.0330827E-4	45.000004	40
ACTAGCA	20	7.0330827E-4	45.000004	14
ACTACGG	20	7.0330827E-4	45.000004	2
TATAGTC	20	7.0330827E-4	45.000004	40
ATACCCG	20	7.0330827E-4	45.000004	11
TCGAAAC	20	7.0330827E-4	45.000004	30
GTTGACG	35	1.211829E-7	45.0	1
CTATACG	25	3.890637E-5	45.0	1
ACGGGTA	135	0.0	45.0	5
CATAGTT	25	3.890637E-5	45.0	36
GTATGCG	55	1.8189894E-12	45.0	1
TACGACG	25	3.890637E-5	45.0	3
CGAATCG	25	3.890637E-5	45.0	2
CTCGATG	35	1.211829E-7	45.0	1
ACGTAGC	25	3.890637E-5	45.0	19
ATACGAG	50	2.1827873E-11	45.0	1
ATACGAC	25	3.890637E-5	45.0	2
AACACGT	55	1.8189894E-12	45.0	41
CACGACA	30	2.1653705E-6	44.999996	16
CGAATAT	115	0.0	44.999996	14
>>END_MODULE