FastQCFastQC Report
Thu 26 May 2016
SRR1546692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546692_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3942040
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296420.7519456930929164No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC63870.1620227090541953No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.0353766E-445.012
TAATGCG1300.043.269231
AATACGG2400.042.1874962
CGTTTTT181000.040.785911
TTACGAG1200.039.3749961
AGGGCGA45050.039.356276
TAACGGG7700.038.571433
GCGATAT5900.038.5169459
CGTTAGG5650.038.230092
TACGGGA9700.037.809284
GGGCGAT97200.037.4537057
CATACGA12400.037.37903218
CGTAAGG4250.037.0588232
TATACGG3300.036.8181842
ACGCTAG1100.036.818181
ACCGTAT552.752131E-936.8181832
CGGTCTA2950.036.6101731
AACGGGA9050.036.5469634
AGGGTAC17550.036.5384646
ACGTAAG1050.036.428571
Produced by FastQC (version 0.11.5)