##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546692_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3942040
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.351916013028788	31.0	31.0	33.0	30.0	34.0
2	31.747595914805533	31.0	31.0	34.0	30.0	34.0
3	31.83913532079837	31.0	31.0	34.0	30.0	34.0
4	35.62229099653986	37.0	35.0	37.0	33.0	37.0
5	35.541543972156546	37.0	35.0	37.0	33.0	37.0
6	35.59973059634098	37.0	35.0	37.0	33.0	37.0
7	35.96518706050674	37.0	35.0	37.0	35.0	37.0
8	35.9396695619527	37.0	35.0	37.0	35.0	37.0
9	37.65551770149466	39.0	37.0	39.0	35.0	39.0
10	37.098898032490794	39.0	37.0	39.0	33.0	39.0
11	36.86205416484866	39.0	37.0	39.0	32.0	39.0
12	36.36804116650262	38.0	35.0	39.0	32.0	39.0
13	36.21179465454435	39.0	35.0	39.0	32.0	39.0
14	37.24194072104798	39.0	35.0	41.0	32.0	41.0
15	37.427230063621884	39.0	35.0	41.0	32.0	41.0
16	37.49176314801473	39.0	35.0	41.0	32.0	41.0
17	37.4126393948311	39.0	35.0	41.0	32.0	41.0
18	37.294410508264754	39.0	35.0	41.0	32.0	41.0
19	37.21232813467139	39.0	35.0	41.0	32.0	41.0
20	37.05348702702154	39.0	35.0	41.0	32.0	41.0
21	36.93646462237826	39.0	35.0	41.0	32.0	41.0
22	36.84758703615387	38.0	35.0	41.0	31.0	41.0
23	36.75649232377145	38.0	35.0	41.0	31.0	41.0
24	36.632409615326075	38.0	35.0	41.0	31.0	41.0
25	36.518886667816666	38.0	35.0	40.0	31.0	41.0
26	36.366466601049204	38.0	35.0	40.0	31.0	41.0
27	36.257051678826194	38.0	35.0	40.0	30.0	41.0
28	36.17515778632383	38.0	35.0	40.0	30.0	41.0
29	36.191473957646295	38.0	35.0	40.0	30.0	41.0
30	36.15229348256233	38.0	35.0	40.0	30.0	41.0
31	36.064788282209214	38.0	35.0	40.0	30.0	41.0
32	35.92444749419082	38.0	35.0	40.0	30.0	41.0
33	35.77659815729927	38.0	35.0	40.0	29.0	41.0
34	35.599806191717995	38.0	35.0	40.0	28.0	41.0
35	35.474921614189604	38.0	35.0	40.0	27.0	41.0
36	35.31892979269617	38.0	34.0	40.0	26.0	41.0
37	35.25700297307993	38.0	34.0	40.0	26.0	41.0
38	35.17714888737811	38.0	34.0	40.0	26.0	41.0
39	35.082691449097425	38.0	34.0	40.0	26.0	41.0
40	34.94527554261246	37.0	34.0	40.0	25.0	41.0
41	34.87525976398007	37.0	34.0	40.0	24.0	41.0
42	34.8113448874187	37.0	34.0	40.0	24.0	41.0
43	34.70153651408915	37.0	34.0	40.0	24.0	41.0
44	34.56713833446642	37.0	34.0	40.0	24.0	41.0
45	34.454442623616195	37.0	34.0	40.0	23.0	41.0
46	34.43293142636807	36.0	34.0	40.0	23.0	41.0
47	34.38573099207517	36.0	33.0	40.0	23.0	41.0
48	34.305456565636064	36.0	33.0	40.0	23.0	41.0
49	34.212035139166524	36.0	33.0	40.0	24.0	41.0
50	34.12546498767136	36.0	33.0	40.0	24.0	41.0
51	33.75298170490406	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	8.0
12	10.0
13	22.0
14	53.0
15	159.0
16	406.0
17	945.0
18	2071.0
19	3795.0
20	6652.0
21	10593.0
22	15603.0
23	23138.0
24	33741.0
25	48349.0
26	64218.0
27	73315.0
28	78501.0
29	86553.0
30	99068.0
31	118624.0
32	145074.0
33	185371.0
34	303837.0
35	465166.0
36	298544.0
37	370658.0
38	539917.0
39	966963.0
40	682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.86042759586407	23.894227354364745	26.554093819443743	14.691251230327445
2	33.83978853588497	24.625878986514596	27.611312924272713	13.923019553327718
3	29.528594331868778	25.235233533906303	31.572054063378353	13.664118070846568
4	25.740073667441223	27.624681636918957	30.862725898265875	15.77251879737395
5	23.34737851467768	31.779738409554444	29.813117066290552	15.059766009477327
6	21.984962100841187	40.05654432730261	27.421335146269442	10.537158425586753
7	85.5210500147132	4.3549532729246785	8.244157847206015	1.879838865156112
8	85.13901431746	3.9412081054479406	8.297632697790991	2.6221448793010724
9	79.15591927022557	5.892126919057137	11.118760844638818	3.8331929660784767
10	42.699744294832115	26.03862467148989	17.78363486925551	13.477996164422482
11	35.403040050329274	25.101292731682072	24.518624874430497	14.977042343558159
12	31.41865633022496	23.478351310488986	28.161231240677413	16.94176111860864
13	22.88726141794604	31.853634158963377	29.134864181997138	16.124240241093442
14	18.620790250733123	33.82086432405556	30.751108563079015	16.807236862132296
15	18.077213828373125	24.483490781422816	40.88667289017869	16.552622500025368
16	20.38279672453856	22.32027072277298	38.4876865785228	18.809245974165663
17	20.747430264533083	22.86087913872005	29.90862599060385	26.483064606143014
18	23.569877525342207	22.99053282057006	33.66739048817364	19.772199165914095
19	27.48498239490213	25.923151464723848	27.022176335095534	19.569689805278482
20	28.978270134245214	24.031719617254012	27.280722671510183	19.709287576990594
21	23.99437854511877	27.370295582997635	29.380955038507985	19.25437083337561
22	24.339453683879412	24.161829915475234	27.753827967245385	23.744888433399964
23	21.48636239104626	28.09423547198912	27.05933983419752	23.360062302767094
24	21.258155675741495	25.153194792543964	34.98016255542815	18.60848697628639
25	21.141439457742692	26.071145904151148	32.08181550669197	20.705599131414193
26	19.9253432233057	29.72694341001106	28.80447686984404	21.5432364968392
27	20.12655883755619	29.79320351899017	29.27073292001096	20.809504723442686
28	18.884536940264432	28.322518290022426	33.25752655985226	19.535418209860882
29	19.575169201733114	25.40065549816846	32.95664173879514	22.067533561303286
30	20.916809570679142	28.616883644001582	30.77492871711094	19.69137806820834
31	25.619729886048848	27.641830118415843	26.7077705959351	20.030669399600207
32	26.023607066392017	27.316085072703473	27.041404957839088	19.61890290306542
33	25.09314973972867	28.194691073657296	25.89836734279713	20.813791843816905
34	20.383659222128646	29.0970411259145	28.113869975951538	22.40542967600532
35	20.565747683940295	28.83032642997027	29.430472547209057	21.17345333888038
36	25.881015920690807	28.428833801787906	26.17005408367241	19.52009619384887
37	20.8660490507453	31.68100780306643	28.169222027173745	19.283721119014523
38	21.794045722519304	31.17185518158111	26.328956580856612	20.705142515042972
39	21.071450315065295	30.342639851447473	27.221844527199114	21.364065306288115
40	24.5759048614423	26.45962496575377	27.663976012419965	21.300494160383966
41	19.69485342614484	26.302371361021198	29.087452182118902	24.91532303071506
42	22.398732635894106	26.552698602753903	26.984023500522575	24.06454526082942
43	22.105026838895597	26.491461273858206	28.35455753873629	23.04895434850991
44	21.18266176903329	28.588167547767146	28.487610475794256	21.741560207405303
45	19.53044616492983	31.723904374384837	26.58423049994419	22.161418960741138
46	22.258018690830127	29.650028919036842	27.109872045945753	20.982080344187274
47	21.296612921228604	28.136903735121916	28.074017513774596	22.492465829874885
48	21.944881330478637	26.865227141277103	29.614641150267374	21.575250377976886
49	21.58250550476403	26.31551176548183	30.21699424663372	21.884988483120416
50	20.809301782833252	30.022754715832413	28.38687583078812	20.781067670546214
51	20.06664062262179	30.271407697537317	26.74188998589563	22.920061693945268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4412.0
1	5620.0
2	6828.0
3	27635.0
4	48442.0
5	34108.0
6	19774.0
7	20122.0
8	20470.0
9	21742.5
10	23015.0
11	23849.5
12	24684.0
13	24462.0
14	24240.0
15	23652.0
16	23064.0
17	22678.0
18	22292.0
19	22008.0
20	21724.0
21	21780.0
22	21836.0
23	23170.0
24	24504.0
25	26069.5
26	32221.0
27	36807.0
28	43046.0
29	49285.0
30	56736.5
31	64188.0
32	74655.0
33	85122.0
34	97239.5
35	109357.0
36	120942.0
37	132527.0
38	147610.5
39	162694.0
40	200084.5
41	237475.0
42	269216.5
43	300958.0
44	318439.0
45	335920.0
46	338322.0
47	340724.0
48	319910.0
49	299096.0
50	281679.0
51	264262.0
52	249721.5
53	235181.0
54	216017.0
55	196853.0
56	181612.0
57	166371.0
58	150848.0
59	135325.0
60	124099.5
61	112874.0
62	105353.5
63	97833.0
64	87751.0
65	77669.0
66	68690.5
67	59712.0
68	50570.5
69	41429.0
70	35293.0
71	29157.0
72	25802.5
73	22448.0
74	18430.5
75	12260.0
76	10107.0
77	7835.5
78	5564.0
79	4329.5
80	3095.0
81	2289.5
82	1484.0
83	1063.0
84	642.0
85	452.0
86	262.0
87	198.0
88	134.0
89	110.0
90	86.0
91	60.5
92	35.0
93	28.0
94	21.0
95	15.0
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3942040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.85189639452219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6634104863162	16.01321667803164
2	7.2183786627337545	2.8659701069804018
3	2.463756992384648	1.4673074566229884
4	1.22962009241715	0.9764116271715225
5	0.7789426150758886	0.7731744045882359
6	0.5751635528088265	0.6850852356159669
7	0.43963388067156367	0.6109296375429529
8	0.3693735450106669	0.586621227714331
9	0.30549708632291533	0.5458226855859825
>10	3.442887476249086	16.142052562653923
>50	1.322195270828336	19.02335918184233
>100	1.1847570288337819	38.31455840834729
>500	0.0044292357511745775	0.5821657717365698
>1k	0.0016935313166255737	0.48156496093050805
>5k	1.3027163974042874E-4	0.1651767040149914
>10k+	1.3027163974042874E-4	0.7665833506203813
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29642	0.7519456930929164	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6387	0.1620227090541953	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.61027285364938E-5	0.0	0.0	0.0367576178831265	0.0
2	7.61027285364938E-5	0.0	0.0	0.12295664173879514	0.0
3	7.61027285364938E-5	0.0	0.0	0.19695386145244595	0.0
4	7.61027285364938E-5	0.0	0.0	0.3529390873760794	0.0
5	7.61027285364938E-5	0.0	0.0	0.5982689166015566	0.0
6	7.61027285364938E-5	0.0	0.0	1.1460056214548813	0.0
7	7.61027285364938E-5	0.0	0.0	1.426444176111861	0.0
8	7.61027285364938E-5	0.0	0.0	2.1789225883045327	0.0
9	7.61027285364938E-5	0.0	0.0	2.540435916429057	0.0
10	7.61027285364938E-5	0.0	0.0	3.0586954977625798	0.0
11	7.61027285364938E-5	0.0	0.0	3.413638623656787	0.0
12	7.61027285364938E-5	0.0	0.0	3.7221337175675537	0.0
13	7.61027285364938E-5	0.0	0.0	3.8801483495854936	0.0
14	7.61027285364938E-5	0.0	0.0	3.955870564479305	0.0
15	7.61027285364938E-5	0.0	0.0	4.0303751357165325	0.0
16	7.61027285364938E-5	0.0	0.0	4.161804547899058	0.0
17	7.61027285364938E-5	0.0	0.0	4.303254152672221	0.0
18	7.61027285364938E-5	0.0	0.0	4.512764964333188	0.0
19	7.61027285364938E-5	0.0	0.0	4.609669105336323	0.0
20	7.61027285364938E-5	0.0	0.0	4.712230215827338	0.0
21	7.61027285364938E-5	0.0	0.0	4.842391249200921	0.0
22	7.61027285364938E-5	0.0	0.0	4.980593804223194	0.0
23	7.61027285364938E-5	0.0	0.0	5.140308063845116	0.0
24	7.61027285364938E-5	0.0	0.0	5.271610638146746	0.0
25	7.61027285364938E-5	0.0	0.0	5.382593783929133	0.0
26	7.61027285364938E-5	0.0	0.0	5.488782457813721	0.0
27	7.61027285364938E-5	0.0	0.0	5.606969995230895	0.0
28	7.61027285364938E-5	0.0	0.0	5.722214893811326	0.0
29	7.61027285364938E-5	0.0	0.0	5.858489513044008	0.0
30	7.61027285364938E-5	0.0	0.0	6.04207466185021	0.0
31	7.61027285364938E-5	0.0	0.0	6.185198526651176	0.0
32	7.61027285364938E-5	0.0	0.0	6.333725685178233	0.0
33	7.61027285364938E-5	0.0	0.0	6.47791498817871	0.0
34	7.61027285364938E-5	0.0	0.0	6.629232580085438	0.0
35	7.61027285364938E-5	0.0	0.0	6.804015179957585	0.0
36	7.61027285364938E-5	0.0	0.0	6.954241966088624	0.0
37	7.61027285364938E-5	0.0	0.0	7.105889336485678	0.0
38	7.61027285364938E-5	0.0	0.0	7.290134042272529	0.0
39	7.61027285364938E-5	0.0	0.0	7.56468731925602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.0353766E-4	45.0	12
TAATGCG	130	0.0	43.26923	1
AATACGG	240	0.0	42.187496	2
CGTTTTT	18100	0.0	40.78591	1
TTACGAG	120	0.0	39.374996	1
AGGGCGA	4505	0.0	39.35627	6
TAACGGG	770	0.0	38.57143	3
GCGATAT	590	0.0	38.516945	9
CGTTAGG	565	0.0	38.23009	2
TACGGGA	970	0.0	37.80928	4
GGGCGAT	9720	0.0	37.453705	7
CATACGA	1240	0.0	37.379032	18
CGTAAGG	425	0.0	37.058823	2
TATACGG	330	0.0	36.818184	2
ACGCTAG	110	0.0	36.81818	1
ACCGTAT	55	2.752131E-9	36.81818	32
CGGTCTA	295	0.0	36.61017	31
AACGGGA	905	0.0	36.546963	4
AGGGTAC	1755	0.0	36.538464	6
ACGTAAG	105	0.0	36.42857	1
>>END_MODULE