##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546691_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	651968
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38210617699028	31.0	31.0	33.0	30.0	34.0
2	31.786466513693924	31.0	31.0	34.0	30.0	34.0
3	31.89914689064494	31.0	31.0	34.0	30.0	34.0
4	35.65113931972121	37.0	35.0	37.0	33.0	37.0
5	35.5673253902032	37.0	35.0	37.0	33.0	37.0
6	35.63132393000883	37.0	35.0	37.0	33.0	37.0
7	35.963599440463334	37.0	35.0	37.0	35.0	37.0
8	35.938395749484634	37.0	35.0	37.0	35.0	37.0
9	37.652587243545696	39.0	37.0	39.0	35.0	39.0
10	37.13978140031413	39.0	37.0	39.0	33.0	39.0
11	36.8934686978502	39.0	37.0	39.0	33.0	39.0
12	36.221504736428784	38.0	35.0	39.0	32.0	39.0
13	35.97697433002847	38.0	35.0	39.0	32.0	39.0
14	36.997668597231765	39.0	35.0	41.0	32.0	41.0
15	37.225912928241875	39.0	35.0	41.0	32.0	41.0
16	37.31993748159419	39.0	35.0	41.0	32.0	41.0
17	37.252046112692646	39.0	35.0	41.0	32.0	41.0
18	37.15997871061156	39.0	35.0	41.0	32.0	41.0
19	37.102911492588596	38.0	35.0	41.0	32.0	41.0
20	36.964725262589575	38.0	35.0	41.0	32.0	41.0
21	36.84807996711495	38.0	35.0	41.0	32.0	41.0
22	36.71157633503485	38.0	35.0	41.0	31.0	41.0
23	36.64217415578679	38.0	35.0	41.0	31.0	41.0
24	36.524117748110335	38.0	35.0	40.0	31.0	41.0
25	36.41223035486404	38.0	35.0	40.0	31.0	41.0
26	36.24955059144007	38.0	35.0	40.0	31.0	41.0
27	36.16000478551094	38.0	35.0	40.0	30.0	41.0
28	36.09022528713066	38.0	35.0	40.0	30.0	41.0
29	36.12506135270443	38.0	35.0	40.0	30.0	41.0
30	36.11061432462943	38.0	35.0	40.0	30.0	41.0
31	36.02045499165603	38.0	35.0	40.0	30.0	41.0
32	35.930278786688916	38.0	35.0	40.0	30.0	41.0
33	35.843142608226174	38.0	35.0	40.0	30.0	41.0
34	35.728261203003825	38.0	35.0	40.0	29.0	41.0
35	35.62574543535879	38.0	35.0	40.0	29.0	41.0
36	35.47706635908511	38.0	35.0	40.0	28.0	41.0
37	35.40625460145283	38.0	34.0	40.0	28.0	41.0
38	35.33176628300775	37.0	34.0	40.0	28.0	41.0
39	35.258488146657506	37.0	34.0	40.0	27.0	41.0
40	35.07826611122018	37.0	34.0	40.0	27.0	41.0
41	35.02412541719839	37.0	34.0	40.0	26.0	41.0
42	34.95789517276921	37.0	34.0	40.0	26.0	41.0
43	34.844187138019045	36.0	34.0	40.0	26.0	41.0
44	34.67814371257485	36.0	34.0	40.0	26.0	41.0
45	34.57388552812408	36.0	34.0	40.0	25.0	41.0
46	34.55416523510356	36.0	34.0	40.0	25.0	41.0
47	34.528522872288214	36.0	34.0	40.0	25.0	41.0
48	34.4456997889467	35.0	34.0	40.0	25.0	41.0
49	34.38396669775204	36.0	34.0	40.0	24.0	41.0
50	34.2693583120644	35.0	34.0	40.0	24.0	41.0
51	33.855006687444785	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	1.0
12	2.0
13	5.0
14	6.0
15	27.0
16	75.0
17	130.0
18	352.0
19	606.0
20	1044.0
21	1690.0
22	2420.0
23	3509.0
24	5094.0
25	6955.0
26	9108.0
27	10697.0
28	12007.0
29	13800.0
30	16061.0
31	19950.0
32	24303.0
33	32427.0
34	55089.0
35	89473.0
36	48191.0
37	59643.0
38	86805.0
39	152400.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.90482968489251	23.03840065770099	27.413615392166484	13.643154265240012
2	32.557119367821734	25.77856581918131	28.8201261411603	12.844188671836655
3	30.686322028074997	25.579783056837147	29.94257386865613	13.791321046431726
4	25.694512614116032	29.158026160793167	29.52460856974575	15.62285265534505
5	23.1031277608717	32.568622999901834	29.206188033768527	15.122061205457937
6	22.267197163050948	40.342010650829494	26.487496318837735	10.90329586728183
7	85.86096250122705	5.194273338568764	7.0748871110238545	1.8698770491803278
8	86.17953641896536	4.16017350544812	6.786836163738097	2.8734539118484346
9	80.67742588593305	5.694758024933739	9.657529203887307	3.9702868852459017
10	47.21934205359772	23.218010699911652	15.620858692451165	13.94178855403946
11	41.0635184548935	24.065598311573574	21.01023363109846	13.860649602434474
12	35.62414106213802	23.584899872386377	24.97499877294591	15.815960292529695
13	22.430702120349466	35.158474035535484	26.138552812407973	16.272271031707078
14	18.303352311769903	37.05565303818592	28.588673063708647	16.052321586335523
15	17.063720918818102	24.172965544321194	42.70316948071071	16.060144056149994
16	18.78972587611662	21.759656915676842	40.43480661627565	19.015810591930894
17	19.98871110238539	21.453200157062923	28.78852949838029	29.769559242171397
18	22.696052566997153	23.64747963090213	32.60911578482379	21.04735201727692
19	29.073666192205756	25.288204329046827	25.6385282713262	19.99960120742122
20	30.36866840090311	24.85213998233042	25.056137724550897	19.72305389221557
21	25.03236355158535	27.849986502405027	27.475121478354765	19.642528467654856
22	23.983078924118974	24.986655786787082	26.36264356532836	24.667621723765585
23	22.059825022086972	29.07826764503779	24.728514282909593	24.133393049965644
24	21.924542308824975	24.435861882791794	34.35828138804358	19.281314420339648
25	20.421707813880435	25.54373834298616	31.98270467262197	22.051849170511435
26	19.133147639147936	32.04620472170413	27.65657210169824	21.16407553744969
27	19.908799204868952	31.272240355354864	28.42056665357809	20.398393786198096
28	18.911204230882497	28.317494110140373	33.784173456365956	18.98712820261117
29	19.271804751153432	25.798352066359087	33.44167198390105	21.488171198586432
30	21.08569745754393	29.425370570334742	29.538412928241875	19.950519043879456
31	27.26192083047021	27.18553671345833	25.931794198488266	19.620748257583195
32	28.89528320408364	27.033228624717776	25.98685825071169	18.084629920486893
33	27.162529449298123	27.06022381466575	25.233600422106605	20.543646313929518
34	21.99479115539413	28.321942181211345	27.99508564837538	21.688181015019143
35	22.489907480121722	26.3600360753902	29.333955040738196	21.816101403749876
36	28.29755448120153	25.964464513595757	26.976784136644742	18.761196868557967
37	23.151903160891333	30.186450868754296	27.50610459409051	19.155541376263866
38	22.90388485324433	29.794407087464414	25.894982575831943	21.40672548345931
39	23.240864582310788	29.172597428094633	26.332580740159027	21.253957249435555
40	24.387086482772162	25.56965986060666	27.600127613625208	22.443126042995974
41	20.78767669578875	24.891098949641698	28.050303082359875	26.27092127220968
42	23.871723765583585	25.691291597133603	26.255429714341812	24.181554922941004
43	22.930113134386964	25.65509350152155	27.893546922548346	23.52124644154314
44	21.743245067242565	28.65094605870227	27.799677284774717	21.806131589280454
45	19.74560101109257	32.08700427014823	25.86246564248552	22.304929076273684
46	22.84115171296751	29.76787204279965	26.946261166192205	20.44471507804064
47	22.660928143712574	27.71976538725827	26.912977324040444	22.70632914498871
48	23.20712059487582	25.510301119073333	29.296990036320803	21.98558824973005
49	22.72764920977717	24.5154670167861	30.336918376362032	22.419965397074705
50	21.444150633159907	29.795020614508687	27.29413100029449	21.466697752036907
51	20.345783842151764	31.142019240208107	25.340200746048886	23.171996171591243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1014.0
1	1163.0
2	1312.0
3	3261.5
4	5211.0
5	3832.0
6	2453.0
7	2528.5
8	2604.0
9	2864.0
10	3124.0
11	3189.0
12	3254.0
13	3279.0
14	3304.0
15	3292.5
16	3281.0
17	3150.0
18	3019.0
19	3045.0
20	3071.0
21	3042.0
22	3013.0
23	3063.0
24	3113.0
25	3474.5
26	4784.5
27	5733.0
28	6794.0
29	7855.0
30	8668.5
31	9482.0
32	10998.5
33	12515.0
34	13581.0
35	14647.0
36	16636.5
37	18626.0
38	22729.0
39	26832.0
40	33096.5
41	39361.0
42	47266.5
43	55172.0
44	54325.0
45	53478.0
46	55288.0
47	57098.0
48	54949.0
49	52800.0
50	50215.0
51	47630.0
52	42907.0
53	38184.0
54	35058.0
55	31932.0
56	30153.0
57	28374.0
58	26047.5
59	23721.0
60	22762.0
61	21803.0
62	19739.0
63	17675.0
64	15262.0
65	12849.0
66	11819.0
67	10789.0
68	9318.0
69	7847.0
70	6853.5
71	5860.0
72	5057.5
73	4255.0
74	3440.0
75	2052.0
76	1479.0
77	1184.5
78	890.0
79	665.5
80	441.0
81	325.0
82	209.0
83	139.5
84	70.0
85	80.5
86	91.0
87	58.0
88	25.0
89	15.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.949165831206226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.98454102551591	19.717354556792053
2	5.962006125893618	3.0941817129496183
3	2.5881037589531206	2.0147740088832817
4	1.6088817913967508	1.6699656163104974
5	1.1641750193108729	1.5104685316322777
6	0.9420701113852091	1.4667560126974628
7	0.8597760287430305	1.561732952328418
8	0.7014888096705311	1.4562439560700862
9	0.6225335610454711	1.453880394995425
>10	8.771103335274164	51.65716071912987
>50	0.7566837899617638	12.265669574254675
>100	0.036853413179598865	1.3525692143790855
>500	5.9440988999353E-4	0.10581166393705697
>1k	0.00118881977998706	0.6734310856402198
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2876	0.4411259448316482	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1490	0.22853882399136152	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	686	0.10521988809266714	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0676352213605574E-4	0.0	0.0	0.03635147737312261	0.0
2	3.0676352213605574E-4	0.0	0.0	0.12454598998723863	0.0
3	3.0676352213605574E-4	0.0	0.0	0.19448807303425936	0.0
4	3.0676352213605574E-4	0.0	0.0	0.3891295278295867	0.0
5	3.0676352213605574E-4	0.0	0.0	0.6277915480514381	0.0
6	3.0676352213605574E-4	0.0	0.0	1.2287412879159714	0.0
7	3.0676352213605574E-4	0.0	0.0	1.513111072936095	0.0
8	3.0676352213605574E-4	0.0	0.0	2.2636080298419556	0.0
9	3.0676352213605574E-4	0.0	0.0	2.6763583488760183	0.0
10	3.0676352213605574E-4	0.0	0.0	3.284363649749681	0.0
11	3.0676352213605574E-4	0.0	0.0	3.674720231667812	0.0
12	3.0676352213605574E-4	0.0	0.0	4.044523657602827	0.0
13	3.0676352213605574E-4	0.0	0.0	4.2295020614508685	0.0
14	3.0676352213605574E-4	0.0	0.0	4.310794394816924	0.0
15	3.0676352213605574E-4	0.0	0.0	4.3815033866692845	0.0
16	3.0676352213605574E-4	0.0	0.0	4.50313512319623	0.0
17	3.0676352213605574E-4	0.0	0.0	4.632742711298714	0.0
18	3.0676352213605574E-4	0.0	0.0	4.839347943457348	0.0
19	3.0676352213605574E-4	0.0	0.0	4.921100422106607	0.0
20	3.0676352213605574E-4	0.0	0.0	5.01650387749092	0.0
21	3.0676352213605574E-4	0.0	0.0	5.128012417787376	0.0
22	3.0676352213605574E-4	0.0	0.0	5.247803573181506	0.0
23	3.0676352213605574E-4	0.0	0.0	5.38277952292137	0.0
24	3.0676352213605574E-4	0.0	0.0	5.487079120447629	0.0
25	3.0676352213605574E-4	0.0	0.0	5.578187886522038	0.0
26	3.0676352213605574E-4	0.0	0.0	5.673744723667419	0.0
27	3.0676352213605574E-4	0.0	0.0	5.7627061450868755	0.0
28	3.0676352213605574E-4	0.0	0.0	5.852741238833808	0.0
29	3.0676352213605574E-4	0.0	0.0	5.963176106802788	0.0
30	3.0676352213605574E-4	0.0	0.0	6.134350152154707	0.0
31	3.0676352213605574E-4	0.0	0.0	6.250153381761068	0.0
32	3.0676352213605574E-4	0.0	0.0	6.36120177677432	0.0
33	3.0676352213605574E-4	0.0	0.0	6.465961519583783	0.0
34	3.0676352213605574E-4	0.0	0.0	6.5793106410130555	0.0
35	3.0676352213605574E-4	0.0	0.0	6.707537793265927	0.0
36	3.0676352213605574E-4	0.0	0.0	6.826562039854717	0.0
37	3.0676352213605574E-4	0.0	0.0	6.94604643172671	0.0
38	3.0676352213605574E-4	0.0	0.0	7.079335182094827	0.0
39	3.0676352213605574E-4	0.0	0.0	7.2604790419161676	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGT	30	2.1640499E-6	45.000004	40
TCGTAAG	30	2.1640499E-6	45.000004	1
CGCAACA	30	2.1640499E-6	45.000004	39
CTTAACG	20	7.031037E-4	45.0	1
CGAACCG	20	7.031037E-4	45.0	45
TCTTACG	20	7.031037E-4	45.0	41
TAACGCC	25	3.888942E-5	45.0	12
CGTTAGC	20	7.031037E-4	45.0	11
TAGCACG	20	7.031037E-4	45.0	1
ACCCGTT	20	7.031037E-4	45.0	19
CCGTTAG	20	7.031037E-4	45.0	10
ATCGCCT	20	7.031037E-4	45.0	14
TACACGG	20	7.031037E-4	45.0	2
CTTACCG	20	7.031037E-4	45.0	1
AAGTCGC	20	7.031037E-4	45.0	22
ATAGGGT	210	0.0	40.714287	4
CACGACC	50	1.0804797E-9	40.5	27
CGTAAGG	50	1.0804797E-9	40.5	2
CGTTTTT	1915	0.0	39.24282	1
GCGCGAC	195	0.0	39.23077	9
>>END_MODULE