FastQCFastQC Report
Thu 26 May 2016
SRR1546690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546690_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3206150
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT756002.357968279712428No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT52380.1633735165229325No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC39750.12398047502456216No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT36560.11403084696598724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG551.8189894E-1245.01
CGTTTTT466150.043.860881
TACGGGA10300.038.44664
GTTTTTT539100.038.4265442
TGGGCGA20150.037.9652636
AGGGCGA31100.037.8376246
AACACGT8850.037.3728841
GGGCGAT65600.037.3170747
GCGAGAC9800.037.1938821
CGTTAGG3650.036.98632
ACACGAC9700.036.64948726
CGACGGT1600.036.562528
GGCGATT17550.036.4102558
GACACGA9750.036.2307725
ACGACCA9700.036.1855728
TAGGGAC24200.036.0743835
CACGACC9800.036.04591827
CTATACG504.8836228E-836.01
TAGGGAT38000.035.7631575
ACGGGAC7250.035.6896555
Produced by FastQC (version 0.11.5)