##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546690_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3206150
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40036367606007	31.0	31.0	33.0	30.0	34.0
2	31.79721379224303	31.0	31.0	34.0	30.0	34.0
3	31.64290348236982	31.0	31.0	34.0	30.0	34.0
4	35.575522355473076	37.0	35.0	37.0	33.0	37.0
5	35.56097001076057	37.0	35.0	37.0	33.0	37.0
6	35.64162874475617	37.0	35.0	37.0	33.0	37.0
7	36.03704692544017	37.0	35.0	37.0	35.0	37.0
8	36.02800180902328	37.0	35.0	37.0	35.0	37.0
9	37.7582555401338	39.0	38.0	39.0	35.0	39.0
10	37.21779985340673	39.0	37.0	39.0	33.0	39.0
11	37.010396893470364	39.0	37.0	39.0	33.0	39.0
12	36.810878779844984	39.0	35.0	39.0	33.0	39.0
13	36.78853765419584	39.0	35.0	39.0	33.0	39.0
14	37.974987134101646	40.0	37.0	41.0	33.0	41.0
15	38.06577171997567	40.0	37.0	41.0	33.0	41.0
16	38.057329819253624	40.0	37.0	41.0	33.0	41.0
17	37.96294153423889	40.0	36.0	41.0	33.0	41.0
18	37.68068524554372	39.0	36.0	41.0	33.0	41.0
19	37.44094786582038	39.0	36.0	41.0	33.0	41.0
20	37.146175007407635	39.0	35.0	41.0	32.0	41.0
21	37.05811955148699	38.0	35.0	41.0	32.0	41.0
22	36.96898647911046	38.0	35.0	41.0	32.0	41.0
23	36.87599769193581	38.0	35.0	41.0	32.0	41.0
24	36.74018277373173	38.0	35.0	41.0	32.0	41.0
25	36.64457464560298	38.0	35.0	40.0	31.0	41.0
26	36.530315799323176	38.0	35.0	40.0	31.0	41.0
27	36.435180824353196	38.0	35.0	40.0	31.0	41.0
28	36.31662929058216	38.0	35.0	40.0	31.0	41.0
29	36.268472155076964	38.0	35.0	40.0	31.0	41.0
30	36.158410554715154	38.0	35.0	40.0	30.0	41.0
31	35.971180075791835	38.0	35.0	40.0	30.0	41.0
32	35.56582318356908	38.0	35.0	40.0	27.0	41.0
33	35.066450415607505	38.0	34.0	40.0	24.0	41.0
34	34.54266207133166	38.0	34.0	40.0	21.0	41.0
35	34.16416293685573	38.0	33.0	40.0	18.0	41.0
36	33.944064688177406	38.0	33.0	40.0	16.0	41.0
37	33.85326263587169	38.0	33.0	40.0	15.0	41.0
38	33.74698813218346	38.0	33.0	40.0	15.0	41.0
39	33.616358248990224	38.0	33.0	40.0	15.0	41.0
40	33.51526035899755	38.0	33.0	40.0	15.0	41.0
41	33.427417931163546	37.0	33.0	40.0	14.0	41.0
42	33.34693635668949	37.0	33.0	40.0	13.0	41.0
43	33.23036726291658	37.0	33.0	40.0	12.0	41.0
44	33.079366218049685	37.0	33.0	40.0	10.0	41.0
45	32.987173089219155	37.0	32.0	40.0	10.0	41.0
46	32.95359636947741	37.0	32.0	40.0	10.0	41.0
47	32.92534722330521	36.0	32.0	40.0	10.0	41.0
48	32.815672067744806	36.0	32.0	40.0	10.0	41.0
49	32.72291876549756	36.0	32.0	40.0	10.0	41.0
50	32.65205776398484	36.0	32.0	40.0	10.0	41.0
51	32.33745832228686	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	1.0
12	3.0
13	15.0
14	21.0
15	66.0
16	260.0
17	672.0
18	1565.0
19	3392.0
20	5998.0
21	9772.0
22	15192.0
23	23523.0
24	38063.0
25	64821.0
26	95209.0
27	102961.0
28	91118.0
29	81880.0
30	81401.0
31	89857.0
32	106271.0
33	132156.0
34	195867.0
35	283681.0
36	255820.0
37	306016.0
38	435802.0
39	784293.0
40	445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.933346849024534	21.80153766979087	24.493707406078943	23.771408075105658
2	40.538277996974564	22.862217924925535	24.457589320524615	12.141914757575286
3	25.648644012288884	22.732997520390498	39.11763329850444	12.500725168816182
4	21.890273380846185	25.369836096252513	39.1096798340689	13.6302106888324
5	20.117991984155452	29.061272866210253	37.7189152098311	13.10181993980319
6	18.397486081437236	36.62885392136987	35.49628058574926	9.477379411443632
7	77.49119660652184	3.217129579090186	17.60344962026106	1.6882241941269125
8	77.65079612619496	2.666063658905541	17.49821436925908	2.18492584564041
9	72.15738502565382	5.183007657158898	19.5255368588494	3.1340704583378822
10	32.46707109773404	29.87190243750292	25.64842568189261	12.012600782870422
11	24.528827409821748	25.19361227640628	35.395599083012335	14.881961230759632
12	22.482385415529528	22.39508444707827	38.077632050902174	17.044898086490026
13	20.102646476303356	25.251563401587575	39.058808851738064	15.586981270371005
14	17.658687210517286	27.50934921946883	38.346272008483695	16.48569156153018
15	16.878561514589148	24.51653852751743	42.85326637867848	15.751633579214946
16	19.49275610935234	23.448060758230277	41.18812282644293	15.871060305974455
17	19.171280195873557	23.627902624643262	37.656628666781025	19.544188512702153
18	20.267298785147293	23.514027727960325	39.12546200271353	17.093211484178845
19	21.788344275844864	25.55825522823324	35.71598334450977	16.93741715141213
20	23.331815417244982	24.570029474603498	35.125680333109806	16.972474775041714
21	21.5177081546403	25.709215102225407	36.352447639692464	16.420629103441822
22	20.720396737520076	23.568547946914524	36.017903092494116	19.693152223071284
23	18.377898725886187	26.9316781809959	35.88584439280758	18.80457870031034
24	18.40986853391139	25.26781342108136	39.251688161814016	17.070629883193238
25	18.91801693620074	26.386039330661387	36.846217425884625	17.849726307253245
26	18.00152831277389	27.661525505668795	36.078817273053346	18.258128908503966
27	18.17020413892051	26.5343168597851	36.743508569468055	18.551970431826334
28	17.37307986213995	26.81175241333063	38.303260920418566	17.51190680411085
29	18.417167007158117	25.25967281630616	37.825678773606974	18.49748140292875
30	18.83137095893829	25.876050715032044	37.96194813093586	17.330630195093804
31	20.38192224318887	26.81343667638757	34.730502315861706	18.07413876456186
32	20.452131060617877	26.565101445659124	34.79728022706361	18.18548726665939
33	19.75534519595153	28.447015891333844	33.13821873586701	18.65942017684762
34	18.207289116229745	28.70414671802629	33.91394663381314	19.17461753193082
35	18.60901704536594	29.356361991797016	32.65009434992125	19.384526612915803
36	20.789856993590444	30.19294169018917	30.518877781763177	18.498323534457214
37	19.171841616892536	30.450602747843984	31.46571433027151	18.91184130499197
38	19.88805888682688	30.529420020897334	30.84007298473247	18.742448107543318
39	19.8226221480592	29.786285732108603	30.2309623691967	20.160129750635498
40	21.44606459460724	28.48672083339831	31.111208146842788	18.95600642515166
41	18.658796375715422	28.668496483321114	31.270277435553545	21.402429705409915
42	20.768179904246526	29.12274846778847	29.912543081265692	20.196528546699312
43	20.42908160878312	28.73037755563526	30.827690532258316	20.0128503033233
44	19.910765248038924	29.827144706267646	30.473496249395694	19.78859379629774
45	19.01595371395599	30.122452162250674	30.360962525146984	20.50063159864635
46	20.764249957113673	29.573444785802288	29.6618685962915	20.000436660792538
47	19.740592299175024	29.414001216412206	30.860533661868594	19.984872822544173
48	20.213527127551735	28.710104018838795	30.828439093616954	20.247929759992513
49	20.062536063502957	27.84782371380004	31.37563744678197	20.714002775915038
50	19.160800336852613	29.07633766355286	31.71046270448981	20.05239929510472
51	18.823105593936653	29.376635528593486	30.872916114342747	20.927342763127115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2805.0
1	3751.0
2	4697.0
3	60794.5
4	116892.0
5	80426.5
6	43961.0
7	44112.5
8	44264.0
9	47023.5
10	49783.0
11	51742.0
12	53701.0
13	53260.5
14	52820.0
15	50687.0
16	48554.0
17	45552.0
18	42550.0
19	39922.5
20	37295.0
21	36383.0
22	35471.0
23	34264.0
24	33057.0
25	33572.0
26	36840.5
27	39594.0
28	43809.0
29	48024.0
30	55089.0
31	62154.0
32	70226.0
33	78298.0
34	87968.0
35	97638.0
36	105500.0
37	113362.0
38	122974.0
39	132586.0
40	147490.5
41	162395.0
42	182508.0
43	202621.0
44	220785.5
45	238950.0
46	241504.0
47	244058.0
48	231565.0
49	219072.0
50	202678.0
51	186284.0
52	174048.5
53	161813.0
54	150187.0
55	138561.0
56	126480.0
57	114399.0
58	104269.5
59	94140.0
60	84834.0
61	75528.0
62	67393.0
63	59258.0
64	52034.5
65	44811.0
66	38120.0
67	31429.0
68	26343.5
69	21258.0
70	17887.0
71	14516.0
72	12237.0
73	9958.0
74	8461.0
75	5975.5
76	4987.0
77	3436.5
78	1886.0
79	1382.5
80	879.0
81	657.0
82	435.0
83	304.5
84	174.0
85	116.5
86	59.0
87	59.0
88	59.0
89	53.5
90	48.0
91	27.5
92	7.0
93	6.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3206150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.663438813121466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.14997359106162	22.588700822392717
2	7.242333299424946	4.296650213834479
3	3.3176521175392444	2.952389117755447
4	2.057920486830942	2.4417999377351514
5	1.42339190636615	2.1111349360792304
6	1.027333216483019	1.8284541604698779
7	0.8273833731148489	1.7180125244370459
8	0.6882984931782512	1.6333840190045406
9	0.5628029236905747	1.502520308166711
>10	5.943893789046069	38.62772728352079
>50	0.6811778862140485	13.1308081507615
>100	0.07408607678744268	3.331144101131965
>500	0.0021444801501776267	0.4535900416166381
>1k	0.0013939120976137612	0.81252445152012
>5k	1.0722400750875088E-4	0.16660154533243515
>10k+	1.0722400750875088E-4	2.404558386241332
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75600	2.357968279712428	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTT	5238	0.1633735165229325	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	3975	0.12398047502456216	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCT	3656	0.11403084696598724	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.238011321990549E-5	0.0	0.0	0.055986151614865184	0.0
2	6.238011321990549E-5	0.0	0.0	0.17007937869407233	0.0
3	6.238011321990549E-5	0.0	0.0	0.31211889649579716	0.0
4	6.238011321990549E-5	0.0	0.0	0.47468147154687085	0.0
5	6.238011321990549E-5	0.0	0.0	0.8379520608829905	0.0
6	6.238011321990549E-5	0.0	0.0	1.5890398141072626	0.0
7	6.238011321990549E-5	0.0	0.0	2.0466915147450995	0.0
8	6.238011321990549E-5	0.0	0.0	2.8789669853250786	0.0
9	6.238011321990549E-5	0.0	0.0	3.35973051791089	0.0
10	6.238011321990549E-5	0.0	0.0	3.9660028382951515	0.0
11	6.238011321990549E-5	0.0	0.0	4.303354490588401	0.0
12	6.238011321990549E-5	0.0	0.0	4.629009871652917	0.0
13	6.238011321990549E-5	0.0	0.0	4.834084493863356	0.0
14	6.238011321990549E-5	0.0	0.0	4.952481948754737	0.0
15	6.238011321990549E-5	0.0	0.0	5.044211905244608	0.0
16	6.238011321990549E-5	0.0	0.0	5.182758136706018	0.0
17	6.238011321990549E-5	0.0	0.0	5.32651310762129	0.0
18	6.238011321990549E-5	0.0	0.0	5.57740592299175	0.0
19	6.238011321990549E-5	0.0	0.0	5.682578793880511	0.0
20	6.238011321990549E-5	0.0	0.0	5.8064968887918536	0.0
21	6.238011321990549E-5	0.0	0.0	5.9365906149119665	0.0
22	6.238011321990549E-5	0.0	0.0	6.074575425354397	0.0
23	6.238011321990549E-5	0.0	0.0	6.2357344478580226	0.0
24	6.238011321990549E-5	0.0	0.0	6.370693822809288	0.0
25	6.238011321990549E-5	0.0	0.0	6.48831152628542	0.0
26	6.238011321990549E-5	0.0	0.0	6.602997364440217	0.0
27	6.238011321990549E-5	0.0	0.0	6.734525833164387	0.0
28	6.238011321990549E-5	0.0	0.0	6.862093164699094	0.0
29	6.238011321990549E-5	0.0	0.0	7.00341531119879	0.0
30	6.238011321990549E-5	0.0	0.0	7.206337819503142	0.0
31	6.238011321990549E-5	0.0	0.0	7.357141743212264	0.0
32	6.238011321990549E-5	0.0	0.0	7.513996537903716	0.0
33	6.238011321990549E-5	0.0	0.0	7.670289911576189	0.0
34	6.238011321990549E-5	0.0	0.0	7.824555931569016	0.0
35	6.238011321990549E-5	0.0	0.0	7.995227921338677	0.0
36	6.238011321990549E-5	0.0	0.0	8.154234829936216	0.0
37	6.238011321990549E-5	0.0	0.0	8.31692216521373	0.0
38	6.238011321990549E-5	0.0	0.0	8.496452131060618	0.0
39	6.238011321990549E-5	0.0	0.0	8.697284905572104	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	55	1.8189894E-12	45.0	1
CGTTTTT	46615	0.0	43.86088	1
TACGGGA	1030	0.0	38.4466	4
GTTTTTT	53910	0.0	38.426544	2
TGGGCGA	2015	0.0	37.965263	6
AGGGCGA	3110	0.0	37.837624	6
AACACGT	885	0.0	37.37288	41
GGGCGAT	6560	0.0	37.317074	7
GCGAGAC	980	0.0	37.19388	21
CGTTAGG	365	0.0	36.9863	2
ACACGAC	970	0.0	36.649487	26
CGACGGT	160	0.0	36.5625	28
GGCGATT	1755	0.0	36.410255	8
GACACGA	975	0.0	36.23077	25
ACGACCA	970	0.0	36.18557	28
TAGGGAC	2420	0.0	36.074383	5
CACGACC	980	0.0	36.045918	27
CTATACG	50	4.8836228E-8	36.0	1
TAGGGAT	3800	0.0	35.763157	5
ACGGGAC	725	0.0	35.689655	5
>>END_MODULE