FastQCFastQC Report
Thu 26 May 2016
SRR1546689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546689_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1243328
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156551.2591206825552066No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14020.11276187779893962No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG207.033509E-445.0000041
GTCGGTC207.033509E-445.00000421
CGTTTTT94300.043.019621
CGAATAT650.041.5384614
GCGATAT1850.041.3513539
ACGGGTA1950.040.3846175
AGGGCGA13550.039.5202946
CGGTCTA403.4585173E-739.37500431
GCGATAC1100.038.8636369
CGTTCGG2100.038.5714345
CGGGTAC1700.038.382356
CGGGATA1700.038.382356
CGTAAGG1650.038.1818162
GTTAGCG950.037.8947371
TAGGGCG4950.037.7272765
CACGACC2150.037.6744227
GGGCGAT28750.037.5652167
GGCGATA6600.037.58
CGCATCG301.1399758E-437.49999621
TCTAGCG301.1399758E-437.4999961
Produced by FastQC (version 0.11.5)