FastQCFastQC Report
Thu 26 May 2016
SRR1546688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546688_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2457308
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC109890.44719668840861626No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104070.42351223371266444No Hit
GAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT32910.13392704536834618Illumina PCR Primer Index 7 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28750.11699795060285482No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT27120.11036467549041472No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT26940.1096321665822925TruSeq Adapter, Index 23 (96% over 26bp)
AAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT25760.10483016374015791TruSeq Adapter, Index 23 (95% over 23bp)
AAGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT25190.1025105521977709TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGA207.034855E-445.00000412
TACGTTC406.8212103E-945.00000440
TCGTTAT850.045.011
TACCCGT750.045.014
CAAGTCG453.8562575E-1045.024
CCGTCGA253.8921073E-545.045
CGTTATG750.045.012
GCTCGAC253.8921073E-545.039
GTACGTC453.8562575E-1045.08
AGTCACG1250.043.1999971
TAATACG850.042.352941
ATTTACG700.041.7857131
TTTACGG2800.041.7857132
ATTAGCG1350.041.6666681
AGGTACG650.041.538461
TTAGCGA650.041.5384627
TCGATAG650.041.538461
ACCCGTA650.041.5384615
CGTTTTT79650.041.3841821
TAGGGTA10450.041.3397145
Produced by FastQC (version 0.11.5)