##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546688_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2457308
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330354599423433	31.0	31.0	33.0	30.0	34.0
2	31.718395496209673	31.0	31.0	34.0	30.0	34.0
3	31.821425315833423	31.0	31.0	34.0	30.0	34.0
4	35.56880741038567	37.0	35.0	37.0	33.0	37.0
5	35.465044267955015	37.0	35.0	37.0	33.0	37.0
6	35.52748943152425	37.0	35.0	37.0	33.0	37.0
7	35.947739558899414	37.0	35.0	37.0	35.0	37.0
8	35.934419698304	37.0	35.0	37.0	35.0	37.0
9	37.658240236877106	39.0	37.0	39.0	35.0	39.0
10	37.10551628041743	39.0	37.0	39.0	33.0	39.0
11	36.82113882346047	39.0	35.0	39.0	32.0	39.0
12	35.89665560849515	37.0	35.0	39.0	32.0	39.0
13	35.498519111157414	37.0	35.0	39.0	31.0	39.0
14	36.428696362035204	38.0	35.0	41.0	31.0	41.0
15	36.761773045951095	38.0	35.0	41.0	32.0	41.0
16	36.92581922982386	38.0	35.0	41.0	32.0	41.0
17	36.88504900484595	38.0	35.0	41.0	32.0	41.0
18	36.81395657361633	38.0	35.0	40.0	32.0	41.0
19	36.74465471971767	38.0	35.0	40.0	32.0	41.0
20	36.58455431716334	37.0	35.0	40.0	32.0	41.0
21	36.44028139736655	37.0	35.0	40.0	31.0	41.0
22	36.323975260732475	37.0	35.0	40.0	31.0	41.0
23	36.28697460798565	37.0	35.0	40.0	31.0	41.0
24	36.18121049538764	37.0	35.0	40.0	31.0	41.0
25	36.101678747637656	36.0	35.0	40.0	31.0	41.0
26	35.971610803366936	36.0	35.0	40.0	31.0	41.0
27	35.899605584647915	36.0	35.0	40.0	31.0	41.0
28	35.86368375474299	36.0	35.0	40.0	30.0	41.0
29	35.90022170602953	36.0	35.0	40.0	31.0	41.0
30	35.88370932744288	36.0	35.0	40.0	30.0	41.0
31	35.71665904314803	36.0	35.0	40.0	30.0	41.0
32	35.55751130912364	36.0	34.0	40.0	30.0	41.0
33	35.38716310694467	36.0	34.0	40.0	29.0	41.0
34	35.30030545621469	36.0	34.0	40.0	29.0	41.0
35	35.175696738056445	36.0	34.0	40.0	29.0	41.0
36	35.03092001491063	36.0	34.0	40.0	27.0	41.0
37	34.95543497192863	36.0	34.0	40.0	27.0	41.0
38	34.92311708585167	36.0	34.0	40.0	27.0	41.0
39	34.882111644124386	36.0	34.0	40.0	27.0	41.0
40	34.71661224396779	36.0	34.0	40.0	26.0	41.0
41	34.704375275708216	36.0	34.0	40.0	26.0	41.0
42	34.64386922599853	36.0	34.0	40.0	26.0	41.0
43	34.50649613316686	35.0	34.0	40.0	26.0	41.0
44	34.32609912961664	35.0	34.0	40.0	24.0	41.0
45	34.20603155973936	35.0	34.0	40.0	24.0	41.0
46	34.18152832286388	35.0	34.0	40.0	24.0	41.0
47	34.15696933392151	35.0	33.0	40.0	24.0	41.0
48	34.10316126427782	35.0	34.0	40.0	23.0	41.0
49	34.10580724923371	35.0	34.0	40.0	24.0	41.0
50	33.9458594527019	35.0	33.0	40.0	24.0	41.0
51	33.54240656848877	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	4.0
13	4.0
14	29.0
15	58.0
16	176.0
17	456.0
18	1061.0
19	2162.0
20	3779.0
21	6048.0
22	9300.0
23	13955.0
24	20541.0
25	29117.0
26	37603.0
27	43749.0
28	47865.0
29	54842.0
30	65252.0
31	79879.0
32	99577.0
33	130069.0
34	232858.0
35	429371.0
36	165202.0
37	193477.0
38	278499.0
39	511970.0
40	402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.281423411310264	25.434784731909875	29.08650441865651	13.197287438123345
2	29.334092429601828	26.8922739843764	30.13130629127484	13.642327294746934
3	28.156909919310074	25.80535284954104	32.16934954836756	13.86838768278132
4	24.49534205724313	29.664698116800984	30.452023108214355	15.38793671774153
5	22.610922196159375	33.257817090897845	30.504397495145092	13.626863217797688
6	20.852819426787363	40.70531654965515	27.135426246933637	11.30643777662385
7	85.66797487331665	4.949033657970429	7.419867594945363	1.9631238737675538
8	85.65409789900167	4.161993531132443	6.433381570401431	3.750526999464455
9	80.20455718208707	6.063261097103009	10.069393010562779	3.6627887102471486
10	49.69039290150034	23.027556985123557	15.028966657822298	12.253083455553801
11	45.27377113491674	20.51330154787271	21.51744917609026	12.695478141120283
12	40.79960672410622	20.94076119070137	24.019292656842367	14.24033942835005
13	21.583456367699938	41.4127980700832	24.298582025533634	12.705163536683232
14	15.049558297128401	44.211795997896886	26.873879871794664	13.864765833180048
15	12.36120990938051	24.79884491484177	49.25316647323005	13.586778702547667
16	13.676714518489339	19.56938242987855	47.91816898817731	18.835734063454804
17	14.517309185498927	20.675674355839803	28.69510049208321	36.11191596657806
18	19.815017083735533	23.3844109081971	35.46559893997822	21.334973068089145
19	26.969594369122635	26.76131766957988	26.565005282203124	19.70408267909436
20	29.82047834459498	23.494165159597415	24.819965588359295	21.86539090744831
21	20.476960967041983	31.16231257945687	27.53586445004045	20.824862003460698
22	20.712177716427895	25.711225454847337	25.47181712670939	28.104779702015374
23	17.58855625749804	32.00986608109362	23.092587498189072	27.30899016321926
24	18.732938646681653	24.563302605941136	38.83815134285161	17.8656074045256
25	16.034335134220047	26.390790246888056	35.245154453572766	22.329720165319124
26	15.3450035567377	35.81358136627561	28.197035129499437	20.644379947487252
27	16.97792869269949	35.783263636467225	27.821705703965478	19.417101966867808
28	14.672072039809416	30.84957197062802	36.74598381643652	17.732372173126038
29	14.566753536797178	26.03316311996705	36.32853512868554	23.07154821455023
30	17.62416432942065	33.37009442853724	29.964497734919675	19.04124350712243
31	28.097942952206235	29.230605198859887	23.655439204202324	19.016012644731553
32	28.53065224220977	29.396762636185613	25.184632939786137	16.887952181818477
33	27.046141550021407	29.975200503966125	23.629475832903324	19.349182113109144
34	19.346740416748734	28.575457370423248	26.98823265134041	25.08956956148761
35	18.10884105696152	28.12561551095752	31.443066965964377	22.32247646611658
36	29.032705708848873	24.8813335568842	27.34996996713477	18.73599076713216
37	18.88993972265585	31.37331583993541	30.643859052263693	19.092885385145046
38	18.54712555365465	32.03249246736673	26.619373721161534	22.80100825781709
39	19.033674248405166	30.718615655831506	28.559220089626535	21.688490006136796
40	23.516995020567222	25.517965187921092	27.69998714039917	23.26505265111252
41	15.918761506494098	24.321900225775522	29.396884721003637	30.36245354672674
42	21.780826823499535	25.70646414694454	25.69966809207474	26.81304093748118
43	22.387629064000116	24.024949253410643	27.76937201197408	25.81804967061516
44	19.037336792945776	30.269587695152584	28.764526058597458	21.928549453304186
45	17.216726596747336	36.357672705253066	24.28372837267449	22.141872325325114
46	22.459496326874774	32.84887364546895	25.454155523035777	19.237474504620504
47	20.972950887719406	27.8434368015731	27.12118301816459	24.062429292542898
48	22.46787948437884	24.17218354394321	31.31829628194756	22.04164068973039
49	21.87088472425923	22.525706993181156	32.17875007935513	23.424658203204483
50	18.89380574189316	31.21147206617974	28.748288777800745	21.146433414126353
51	17.52832774727466	32.41001941962505	25.357464347163646	24.704188485936644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3685.0
1	3991.0
2	4297.0
3	10748.5
4	17200.0
5	12919.0
6	8638.0
7	9645.0
8	10652.0
9	12096.0
10	13540.0
11	14249.0
12	14958.0
13	15119.0
14	15280.0
15	14887.5
16	14495.0
17	13693.5
18	12892.0
19	12489.5
20	12087.0
21	12259.0
22	12431.0
23	12504.5
24	12578.0
25	12763.0
26	14436.0
27	15924.0
28	17685.5
29	19447.0
30	23272.0
31	27097.0
32	33285.0
33	39473.0
34	43175.0
35	46877.0
36	52457.0
37	58037.0
38	76838.5
39	95640.0
40	135486.0
41	175332.0
42	220071.0
43	264810.0
44	288291.5
45	311773.0
46	310786.5
47	309800.0
48	275926.5
49	242053.0
50	210648.5
51	179244.0
52	154854.5
53	130465.0
54	113492.0
55	96519.0
56	86995.5
57	77472.0
58	67948.5
59	58425.0
60	51186.0
61	43947.0
62	38666.0
63	33385.0
64	28698.5
65	24012.0
66	19296.0
67	14580.0
68	12492.0
69	10404.0
70	8599.0
71	6794.0
72	5558.0
73	4322.0
74	3606.5
75	2246.5
76	1602.0
77	1070.0
78	538.0
79	440.5
80	343.0
81	287.0
82	231.0
83	164.0
84	97.0
85	74.5
86	52.0
87	35.5
88	19.0
89	15.5
90	12.0
91	8.0
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2457308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.148639830473876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14624880686783	16.738355099495205
2	8.796061065830225	3.720494548161951
3	3.088437620675182	1.9594876463563535
4	1.5703792563262795	1.328455411571676
5	0.9180242016525253	0.9707481598203791
6	0.6430125649081119	0.8159304685026517
7	0.47226562487913554	0.6991442923417763
8	0.35634190857405307	0.6028917344749039
9	0.2957734149328495	0.5629684881459722
>10	2.7383032395752136	12.918846114400303
>50	0.85619974621951	13.159741829628146
>100	1.1060685895613087	43.05949020162961
>500	0.009174941210503489	1.2021656920005694
>1k	0.0033185957569906235	1.377955434661321
>5k	0.0	0.0
>10k+	3.90423030234191E-4	0.8833248788091688
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10989	0.44719668840861626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10407	0.42351223371266444	No Hit
GAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	3291	0.13392704536834618	Illumina PCR Primer Index 7 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2875	0.11699795060285482	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2712	0.11036467549041472	No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	2694	0.1096321665822925	TruSeq Adapter, Index 23 (96% over 26bp)
AAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	2576	0.10483016374015791	TruSeq Adapter, Index 23 (95% over 23bp)
AAGAGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	2519	0.1025105521977709	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12143369899092829	0.0
2	0.0	0.0	0.0	0.4482547568314595	0.0
3	0.0	0.0	0.0	0.6759836373787901	0.0
4	0.0	0.0	0.0	1.2174298052991321	0.0
5	0.0	0.0	0.0	1.9808668673198475	0.0
6	0.0	0.0	0.0	3.282128247659634	0.0
7	0.0	0.0	0.0	3.890192031279758	0.0
8	0.0	0.0	0.0	5.556364932682431	0.0
9	0.0	0.0	0.0	6.348207062362553	0.0
10	0.0	0.0	0.0	7.581467199064993	0.0
11	0.0	0.0	0.0	8.501294912969803	0.0
12	0.0	0.0	0.0	9.285486394053981	0.0
13	0.0	0.0	0.0	9.650845559449609	0.0
14	0.0	0.0	0.0	9.808090805059846	0.0
15	0.0	0.0	0.0	9.976730633685317	0.0
16	0.0	0.0	0.0	10.319504107747177	0.0
17	0.0	0.0	0.0	10.689421106348899	0.0
18	0.0	0.0	0.0	11.279294252083988	0.0
19	0.0	0.0	0.0	11.501366536063042	0.0
20	0.0	0.0	0.0	11.746024511375863	0.0
21	0.0	0.0	0.0	12.052579489425012	0.0
22	0.0	0.0	0.0	12.34822822373101	0.0
23	0.0	0.0	0.0	12.699751109750995	0.0
24	0.0	0.0	0.0	12.955844363018393	0.0
25	0.0	0.0	0.0	13.164894266408606	0.0
26	0.0	0.0	0.0	13.368450352987903	0.0
27	0.0	0.0	0.0	13.573552847262127	0.0
28	0.0	0.0	0.0	13.768318826943956	0.0
29	0.0	0.0	0.0	13.983472971235189	0.0
30	0.0	0.0	0.0	14.307486078261253	0.0
31	0.0	0.0	0.0	14.568299944492102	0.0
32	0.0	0.0	0.0	14.777716102336377	0.0
33	0.0	0.0	0.0	14.989085617268978	0.0
34	0.0	0.0	0.0	15.208024390918842	0.0
35	0.0	0.0	0.0	15.494150509419251	0.0
36	0.0	0.0	0.0	15.717321556760487	0.0
37	0.0	0.0	0.0	15.943829589127615	0.0
38	0.0	0.0	0.0	16.159472072690928	0.0
39	0.0	0.0	0.0	16.382561730153483	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGA	20	7.034855E-4	45.000004	12
TACGTTC	40	6.8212103E-9	45.000004	40
TCGTTAT	85	0.0	45.0	11
TACCCGT	75	0.0	45.0	14
CAAGTCG	45	3.8562575E-10	45.0	24
CCGTCGA	25	3.8921073E-5	45.0	45
CGTTATG	75	0.0	45.0	12
GCTCGAC	25	3.8921073E-5	45.0	39
GTACGTC	45	3.8562575E-10	45.0	8
AGTCACG	125	0.0	43.199997	1
TAATACG	85	0.0	42.35294	1
ATTTACG	70	0.0	41.785713	1
TTTACGG	280	0.0	41.785713	2
ATTAGCG	135	0.0	41.666668	1
AGGTACG	65	0.0	41.53846	1
TTAGCGA	65	0.0	41.53846	27
TCGATAG	65	0.0	41.53846	1
ACCCGTA	65	0.0	41.53846	15
CGTTTTT	7965	0.0	41.384182	1
TAGGGTA	1045	0.0	41.339714	5
>>END_MODULE