FastQCFastQC Report
Thu 26 May 2016
SRR1546687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546687_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5484352
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1685663.073580980943601No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT126740.2310938466385819No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT96140.17529874085397873No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC95090.17338420291038942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT900200.043.9952241
GTTTTTT1021200.039.101792
TACGGGA16200.037.54
CGACATA301.1404838E-437.49999625
ACGGGTA6200.037.3790365
TATAGCG2350.037.3404271
GCTACGA6600.037.15909210
AGGGCGA58750.036.880856
TAGGGAC41450.036.857665
AGGGTAC25700.036.7704286
GACCGAT33500.036.7388089
CACGACG4300.036.62790326
TAAGGGA54900.036.352464
TAGCATA27650.036.21157530
TATGGGC19200.036.2109384
CGTTCGA2550.036.1764714
ATAACGC2800.036.16071311
TACGAAT6800.036.06617712
CGGTCTA4150.035.78313431
GGCGATA23800.035.7352948
Produced by FastQC (version 0.11.5)