##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546687_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5484352
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287886153186374	31.0	31.0	33.0	30.0	34.0
2	31.666804756514534	31.0	31.0	34.0	30.0	34.0
3	31.457682694327424	31.0	31.0	34.0	30.0	34.0
4	35.443924095317	37.0	35.0	37.0	33.0	37.0
5	35.4332759822856	37.0	35.0	37.0	33.0	37.0
6	35.51940028648781	37.0	35.0	37.0	33.0	37.0
7	35.95716668076739	37.0	35.0	37.0	35.0	37.0
8	35.94355668636878	37.0	35.0	37.0	35.0	37.0
9	37.660799671501756	39.0	37.0	39.0	35.0	39.0
10	37.097310675901184	39.0	37.0	39.0	33.0	39.0
11	36.8828840672517	39.0	37.0	39.0	32.0	39.0
12	36.710036846650254	39.0	35.0	39.0	32.0	39.0
13	36.699932644731774	39.0	35.0	39.0	32.0	39.0
14	37.8819364621381	40.0	37.0	41.0	33.0	41.0
15	37.95828075951361	40.0	37.0	41.0	33.0	41.0
16	37.9470418747739	40.0	37.0	41.0	33.0	41.0
17	37.84866197501546	40.0	36.0	41.0	33.0	41.0
18	37.540184510403414	39.0	36.0	41.0	32.0	41.0
19	37.27235943280081	38.0	36.0	41.0	32.0	41.0
20	36.929372148250145	38.0	35.0	41.0	32.0	41.0
21	36.84621938927334	38.0	35.0	41.0	32.0	41.0
22	36.756237564620214	38.0	35.0	40.0	32.0	41.0
23	36.6521082162487	38.0	35.0	40.0	31.0	41.0
24	36.49135011757086	38.0	35.0	40.0	31.0	41.0
25	36.37573208284224	38.0	35.0	40.0	31.0	41.0
26	36.242091134923506	38.0	35.0	40.0	31.0	41.0
27	36.13157616433081	38.0	35.0	40.0	31.0	41.0
28	35.99509860052746	37.0	35.0	40.0	30.0	41.0
29	35.90738668852765	37.0	35.0	40.0	30.0	41.0
30	35.779013819681886	37.0	35.0	40.0	30.0	41.0
31	35.57526085123639	37.0	35.0	40.0	29.0	41.0
32	35.134485167983385	37.0	34.0	40.0	25.0	41.0
33	34.58765848727434	37.0	33.0	40.0	22.0	41.0
34	33.99900535195407	37.0	33.0	40.0	18.0	41.0
35	33.584818406987736	37.0	33.0	40.0	15.0	41.0
36	33.35778155742009	37.0	33.0	40.0	15.0	41.0
37	33.24998778342455	37.0	33.0	40.0	13.0	41.0
38	33.130728662201115	37.0	33.0	40.0	12.0	41.0
39	32.9986012932795	37.0	32.0	40.0	10.0	41.0
40	32.88841215881111	37.0	32.0	40.0	10.0	41.0
41	32.782025479035624	36.0	32.0	40.0	10.0	41.0
42	32.687330426639285	36.0	32.0	40.0	10.0	41.0
43	32.55937930315195	36.0	31.0	40.0	10.0	41.0
44	32.386244354848124	36.0	31.0	40.0	10.0	41.0
45	32.29849925752395	35.0	31.0	40.0	10.0	41.0
46	32.25230929743386	35.0	31.0	40.0	10.0	41.0
47	32.221850275109986	35.0	31.0	40.0	10.0	41.0
48	32.09001464530358	35.0	31.0	40.0	10.0	41.0
49	31.987679674827582	35.0	31.0	40.0	10.0	41.0
50	31.909968944371187	35.0	30.0	40.0	10.0	41.0
51	31.60560372492502	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	9.0
11	10.0
12	9.0
13	28.0
14	62.0
15	192.0
16	550.0
17	1451.0
18	3601.0
19	7378.0
20	12707.0
21	20378.0
22	30798.0
23	47291.0
24	75158.0
25	126702.0
26	182114.0
27	192184.0
28	168970.0
29	152199.0
30	151824.0
31	167522.0
32	195337.0
33	239080.0
34	341496.0
35	483253.0
36	477801.0
37	535336.0
38	705131.0
39	1165059.0
40	720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.815883444388692	21.019493278330785	23.63818004387756	25.52644323340296
2	42.10754524873677	21.975759396916903	24.093931242925326	11.822764111421002
3	24.888391554736092	22.351118236028615	40.665095894647166	12.095394314588123
4	21.62085876325954	24.480996114034987	40.681250948152126	13.216894174553348
5	19.300475243018685	28.468851014668644	39.156731734214	13.073942008098676
6	17.720106222211847	35.4939289090124	37.145555208710164	9.640409660065583
7	74.7617950124281	3.260385183153817	19.93289271002299	2.044927094395108
8	74.44839426790986	2.981227317283792	19.886323853757016	2.684054561049327
9	68.88474700383928	5.3398833627017375	22.171279305194123	3.604090328264852
10	31.771428967360226	27.52771886268423	28.294409257465603	12.406442912489934
11	23.938215490180063	24.64012156768931	36.36229038544572	15.059372556684911
12	21.83818617039898	21.468643879896838	39.44992954500368	17.2432404047005
13	19.703512830686286	23.554195646085443	40.80312496353261	15.939166559695659
14	17.547925443151716	26.450235141726864	39.55586731121562	16.445972103905802
15	16.74343659925548	24.24534384372119	43.492357893876985	15.518861663146346
16	18.88230368874937	23.626875153163034	41.54554631066715	15.945274847420443
17	18.585076231430804	24.437144078279438	38.30868259367743	18.669097096612326
18	19.458433740212154	24.39279973276697	39.454743240404696	16.69402328661618
19	21.06626270523847	26.857466479175663	35.51371246776283	16.562558347823042
20	22.489019669051146	25.09986594587656	35.28571834922339	17.1253960358489
21	20.824046304832365	26.55456834280513	36.4648184507486	16.1565669016139
22	20.126388678188416	24.45942565320388	36.144981212000985	19.269204456606722
23	18.2484457598637	27.301219907110262	35.81932742464379	18.631006908382247
24	18.432387272005883	25.767073302370086	39.0281294854889	16.772409940135134
25	19.168663864026232	27.085934673777324	36.36763285799307	17.37776860420338
26	17.94820974291949	28.00786674524173	35.97750472617366	18.066418785665107
27	18.07734076879092	27.072077065804674	36.05342983090801	18.7971523344964
28	17.917175994538646	27.332071318544106	37.15520083320691	17.595551853710337
29	18.351593770786412	26.376552781440726	36.52035828480739	18.75149516296547
30	18.74274298950906	27.380135337775545	36.30848275238351	17.568638920331882
31	19.953752056760763	27.76131072549683	33.9399075770483	18.345029640694104
32	19.983855886711865	28.75470064649388	33.213878321449826	18.04756514534443
33	18.97972631953602	28.95999381513076	32.01153025918103	20.048749606152192
34	17.316685726955527	31.055300607984314	32.4204026291529	19.207611035907252
35	17.783741816717818	30.718633669027806	31.02479563674979	20.472828877504583
36	19.346077713465508	32.21824565600458	29.1730727714049	19.262603859125015
37	18.467432433220917	31.910934965516436	30.558596530638443	19.063036070624207
38	18.85374972284784	31.73426869755989	30.11159750504709	19.30038407454518
39	18.632702641989425	31.162186526320703	29.88172531595346	20.32338551573641
40	20.502732136813975	29.524235497648583	30.612896473457575	19.360135892079867
41	18.287082958934803	29.828701731763392	30.783490921078734	21.100724388223075
42	19.7895758696743	30.791659616304717	28.954104331742382	20.4646601822786
43	19.354319343470294	29.4817145216062	31.074555389588415	20.089410745335094
44	20.10820968457167	30.739985325522508	29.6125230473901	19.539281942515725
45	18.920302708505947	30.699761795012424	29.49927356960312	20.880661926878506
46	20.12115560780927	30.574696883059293	28.745529098059354	20.558618411072082
47	19.381943390942084	30.603724925023045	29.744188556824945	20.270143127209924
48	19.652549653997408	29.48572593444039	30.013026151494287	20.848698260067916
49	19.459126620610785	28.96681321694888	30.335051433606015	21.239008728834328
50	18.644937451133696	29.54215921953952	31.52237493144131	20.290528397885474
51	18.2249242936996	29.886593712438593	30.578854165451087	21.309627828410722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4925.0
1	6574.5
2	8224.0
3	121580.5
4	234937.0
5	156975.5
6	79014.0
7	80216.0
8	81418.0
9	88200.0
10	94982.0
11	101854.5
12	108727.0
13	110601.5
14	112476.0
15	109045.0
16	105614.0
17	98555.0
18	91496.0
19	85399.5
20	79303.0
21	74390.5
22	69478.0
23	66673.5
24	63869.0
25	62581.5
26	63426.0
27	65558.0
28	68743.0
29	71928.0
30	80712.5
31	89497.0
32	96863.0
33	104229.0
34	122329.0
35	140429.0
36	150584.5
37	160740.0
38	177158.0
39	193576.0
40	218727.5
41	243879.0
42	275494.0
43	307109.0
44	353940.5
45	400772.0
46	409669.5
47	418567.0
48	399212.5
49	379858.0
50	347833.5
51	315809.0
52	294333.5
53	272858.0
54	253731.0
55	234604.0
56	217325.0
57	200046.0
58	186760.5
59	173475.0
60	157333.0
61	141191.0
62	126415.0
63	111639.0
64	99138.0
65	86637.0
66	74098.5
67	61560.0
68	51050.0
69	40540.0
70	34284.0
71	28028.0
72	23118.5
73	18209.0
74	15440.0
75	10100.5
76	7530.0
77	5927.5
78	4325.0
79	2979.0
80	1633.0
81	1303.0
82	973.0
83	705.0
84	437.0
85	308.0
86	179.0
87	110.0
88	41.0
89	34.5
90	28.0
91	27.0
92	26.0
93	17.0
94	8.0
95	6.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5484352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.11037263784247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43839233902406	23.015884771656175
2	7.750012365108205	4.667115205225898
3	3.566170813110149	3.221361962188328
4	2.1679882202373513	2.611157327431982
5	1.4278445052234336	2.14964650605867
6	1.0390686817788424	1.8772047122803621
7	0.8324912126061581	1.7546634440510533
8	0.6249771351725342	1.5054635544140997
9	0.5104595383636202	1.3833114225002683
>10	4.7395709920557625	31.053350706818918
>50	0.7763507513668312	15.852567603243555
>100	0.12179474359684363	5.435094019307987
>500	0.0028407637455418067	0.5870217788941422
>1k	0.0017909157487444687	1.1604318350487344
>5k	1.2351143094782178E-4	0.35558981478753227
>10k+	1.2351143094782178E-4	3.3701353360922637
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168566	3.073580980943601	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT	12674	0.2310938466385819	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT	9614	0.17529874085397873	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	9509	0.17338420291038942	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6467389401701424E-5	0.0	0.0	0.06753760517195104	0.0
2	3.6467389401701424E-5	0.0	0.0	0.20585841317260453	0.0
3	3.6467389401701424E-5	0.0	0.0	0.3795890562823101	0.0
4	3.6467389401701424E-5	0.0	0.0	0.5795579860665399	0.0
5	3.6467389401701424E-5	0.0	0.0	1.0743292917741238	0.0
6	3.6467389401701424E-5	0.0	0.0	2.1425867632128646	0.0
7	3.6467389401701424E-5	0.0	0.0	2.869710040493389	0.0
8	3.6467389401701424E-5	0.0	0.0	4.105462231454144	0.0
9	3.6467389401701424E-5	0.0	0.0	4.803502765686813	0.0
10	3.6467389401701424E-5	0.0	0.0	5.628303945479794	0.0
11	3.6467389401701424E-5	0.0	0.0	6.065730281353202	0.0
12	3.6467389401701424E-5	0.0	0.0	6.501989660765757	0.0
13	3.6467389401701424E-5	0.0	0.0	6.815007497695261	0.0
14	3.6467389401701424E-5	0.0011487227661535948	0.0	6.997253276230264	0.0
15	3.6467389401701424E-5	0.0011487227661535948	0.0	7.130304546462371	0.0
16	3.6467389401701424E-5	0.0011487227661535948	0.0	7.311255732673614	0.0
17	3.6467389401701424E-5	0.0011487227661535948	0.0	7.484895207309815	0.0
18	3.6467389401701424E-5	0.0011487227661535948	0.0	7.788395055605475	0.0
19	3.6467389401701424E-5	0.0011487227661535948	0.0	7.923379097475873	0.0
20	3.6467389401701424E-5	0.0011487227661535948	0.0	8.074718763492934	0.0
21	3.6467389401701424E-5	0.0011487227661535948	0.0	8.227115983802644	0.0
22	3.6467389401701424E-5	0.0011487227661535948	0.0	8.384253964734576	0.0
23	3.6467389401701424E-5	0.0011487227661535948	0.0	8.568778955107184	0.0
24	3.6467389401701424E-5	0.0011487227661535948	0.0	8.729107832611765	0.0
25	3.6467389401701424E-5	0.0011487227661535948	0.0	8.871677091477716	0.0
26	3.6467389401701424E-5	0.0011487227661535948	0.0	9.016890236075293	0.0
27	3.6467389401701424E-5	0.0011487227661535948	0.0	9.175067537605171	0.0
28	3.6467389401701424E-5	0.0011487227661535948	0.0	9.331713208780181	0.0
29	3.6467389401701424E-5	0.0011487227661535948	0.0	9.50854357998903	0.0
30	5.4701084102552136E-5	0.0011487227661535948	0.0	9.745891583910005	0.0
31	5.4701084102552136E-5	0.0011487227661535948	0.0	9.924800596314752	0.0
32	5.4701084102552136E-5	0.0011487227661535948	0.0	10.111349526799156	0.0
33	5.4701084102552136E-5	0.0011487227661535948	0.0	10.296348593233986	0.0
34	5.4701084102552136E-5	0.0011487227661535948	0.0	10.48222287701446	0.0
35	5.4701084102552136E-5	0.0011487227661535948	0.0	10.676539361441424	0.0
36	5.4701084102552136E-5	0.0011487227661535948	0.0	10.87189516646634	0.0
37	5.4701084102552136E-5	0.0011487227661535948	0.0	11.067232737796553	0.0
38	5.4701084102552136E-5	0.0011487227661535948	0.0	11.286985226331206	0.0
39	5.4701084102552136E-5	0.0011487227661535948	0.0	11.529675702799528	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	90020	0.0	43.995224	1
GTTTTTT	102120	0.0	39.10179	2
TACGGGA	1620	0.0	37.5	4
CGACATA	30	1.1404838E-4	37.499996	25
ACGGGTA	620	0.0	37.379036	5
TATAGCG	235	0.0	37.340427	1
GCTACGA	660	0.0	37.159092	10
AGGGCGA	5875	0.0	36.88085	6
TAGGGAC	4145	0.0	36.85766	5
AGGGTAC	2570	0.0	36.770428	6
GACCGAT	3350	0.0	36.738808	9
CACGACG	430	0.0	36.627903	26
TAAGGGA	5490	0.0	36.35246	4
TAGCATA	2765	0.0	36.211575	30
TATGGGC	1920	0.0	36.210938	4
CGTTCGA	255	0.0	36.17647	14
ATAACGC	280	0.0	36.160713	11
TACGAAT	680	0.0	36.066177	12
CGGTCTA	415	0.0	35.783134	31
GGCGATA	2380	0.0	35.735294	8
>>END_MODULE