##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546686_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1688549
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332540542205173	31.0	31.0	33.0	30.0	34.0
2	31.72612284274842	31.0	31.0	34.0	30.0	34.0
3	31.825499289626773	31.0	31.0	34.0	30.0	34.0
4	35.56382669380634	37.0	35.0	37.0	33.0	37.0
5	35.45918418713345	37.0	35.0	37.0	33.0	37.0
6	35.53030382890873	37.0	35.0	37.0	33.0	37.0
7	35.93024247445588	37.0	35.0	37.0	35.0	37.0
8	35.91541376649419	37.0	35.0	37.0	35.0	37.0
9	37.64621577460885	39.0	37.0	39.0	35.0	39.0
10	37.11491819307583	39.0	37.0	39.0	33.0	39.0
11	36.76651847236888	39.0	35.0	39.0	32.0	39.0
12	35.62118303940247	37.0	35.0	39.0	31.0	39.0
13	35.10182055717661	37.0	35.0	39.0	30.0	39.0
14	35.98536850277961	38.0	35.0	40.0	30.0	41.0
15	36.40136057644759	37.0	35.0	40.0	31.0	41.0
16	36.61667739579959	37.0	35.0	40.0	32.0	41.0
17	36.587733018112	37.0	35.0	40.0	32.0	41.0
18	36.513656399666225	37.0	35.0	40.0	32.0	41.0
19	36.435838699380355	37.0	35.0	40.0	32.0	41.0
20	36.22499968908217	36.0	35.0	40.0	31.0	41.0
21	36.0575802064376	36.0	35.0	40.0	31.0	41.0
22	35.93563349360901	36.0	35.0	40.0	31.0	41.0
23	35.91733671927791	35.0	35.0	40.0	31.0	41.0
24	35.79477172412527	35.0	35.0	40.0	31.0	41.0
25	35.69608048093363	35.0	34.0	40.0	31.0	41.0
26	35.53207398778478	35.0	34.0	40.0	30.0	41.0
27	35.460707980639	35.0	34.0	40.0	30.0	41.0
28	35.46558435674653	36.0	34.0	40.0	30.0	41.0
29	35.51618934363172	36.0	34.0	40.0	30.0	41.0
30	35.513503013534105	36.0	35.0	40.0	30.0	41.0
31	35.340251304522404	35.0	34.0	40.0	30.0	41.0
32	35.11757550417548	35.0	34.0	40.0	29.0	41.0
33	34.939146569036495	35.0	34.0	40.0	29.0	41.0
34	34.812131599379114	35.0	34.0	40.0	27.0	41.0
35	34.68121742395394	35.0	34.0	40.0	27.0	41.0
36	34.46032540364538	35.0	34.0	40.0	25.0	41.0
37	34.377972448534216	35.0	34.0	40.0	25.0	41.0
38	34.36764583082872	35.0	33.0	40.0	26.0	41.0
39	34.33889214941349	35.0	34.0	40.0	25.0	41.0
40	34.126230864487795	35.0	33.0	40.0	23.0	41.0
41	34.13459188924929	35.0	34.0	40.0	23.0	41.0
42	34.05543753838355	35.0	33.0	40.0	23.0	41.0
43	33.90757863704281	35.0	33.0	40.0	23.0	41.0
44	33.78051332830732	35.0	33.0	39.0	23.0	41.0
45	33.69020679885511	35.0	33.0	39.0	23.0	41.0
46	33.682610928080855	35.0	33.0	39.0	23.0	41.0
47	33.65551665956984	35.0	33.0	39.0	23.0	41.0
48	33.62354009270682	35.0	33.0	39.0	23.0	41.0
49	33.606499426430624	35.0	33.0	39.0	24.0	41.0
50	33.4429430238625	35.0	33.0	39.0	23.0	41.0
51	33.0236972690754	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	8.0
12	3.0
13	11.0
14	17.0
15	47.0
16	155.0
17	425.0
18	965.0
19	1919.0
20	3293.0
21	5151.0
22	7882.0
23	11446.0
24	16470.0
25	22984.0
26	29687.0
27	34211.0
28	36662.0
29	40394.0
30	47262.0
31	57307.0
32	70759.0
33	93900.0
34	175704.0
35	332641.0
36	104494.0
37	119107.0
38	170945.0
39	304385.0
40	312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.60308110691487	23.69732829784626	29.33169247679517	14.367898118443707
2	29.772485133685784	26.930636895938463	30.361926127106763	12.934951843268985
3	28.198411772474476	26.176912840551264	31.895136001383435	13.729539385590824
4	24.156657579969547	29.23178421236221	31.935525708759414	14.676032498908825
5	23.18416581336994	31.945652746825825	30.97843177781634	13.891749661987895
6	20.86400809215486	40.87924010496587	27.893238514250996	10.363513288628285
7	85.21979522063026	5.437331104990142	7.531969756281873	1.8109039180977275
8	86.14011201333216	4.387613270328548	6.671526855305947	2.800747861033349
9	81.61782690345379	5.5371801469782636	9.312374115290702	3.5326188342772404
10	55.32637785459586	21.262693590769352	12.89444369100334	10.516484863631437
11	50.77916009544289	18.602717481103596	19.404826273919205	11.213296149534305
12	45.84285087373834	20.37897626897413	21.252033550699448	12.526139306588083
13	20.94016815620986	45.205676589782115	21.662445093390833	12.191710160617193
14	13.412047858842119	48.08909898380207	25.200275502813362	13.29857765454245
15	11.403873977006294	23.383094005563358	52.583786434388344	12.629245583042009
16	12.216228252777976	16.853404905631994	51.60501708863645	19.32534975295357
17	13.703363064974722	18.664190378840058	28.515784854333514	39.116661701851704
18	19.847277159265143	23.011591609127127	35.835086811220755	21.30604442038697
19	29.298527907688793	25.283068480689636	25.319549506706647	20.098854104914928
20	32.42938167622023	22.157603954637974	24.186742581944618	21.226271787197174
21	20.085292165048216	30.545574928533313	27.904905335883058	21.464227570535414
22	21.387652949366586	25.567750773000963	24.167080730260125	28.87751554737233
23	17.732443654285422	33.11713192806368	22.433698992448544	26.71672542520235
24	19.544117464165982	22.963147649253887	40.46065586488755	17.03207902169259
25	14.642927152247283	25.64770107352526	37.623190088057854	22.086181686169603
26	14.553027480991076	37.04144801246514	27.95595508332894	20.449569423214843
27	16.17311668183748	37.51783335870028	27.85504003733383	18.454009922128407
28	13.026213630756347	30.32005585860997	40.05083654664448	16.602893963989203
29	13.685063329521382	23.983076594164576	40.19143063067758	22.14042944563646
30	17.469436776782906	32.85750072991663	32.173363047207985	17.499699446092475
31	29.31866353893195	28.174722794541353	23.862558918929803	18.644054747596904
32	31.07046345708653	27.511668302193186	24.85820666145904	16.55966157926125
33	29.175522889771038	28.337584517831583	23.109427087990934	19.377465504406448
34	19.389073103593677	28.60142050956176	26.930400006159132	25.079106380685428
35	19.480867893084536	26.170398371619658	31.44220274330209	22.90653099199372
36	32.24194263832438	24.152630453720917	26.409360936520056	17.196065971434646
37	19.6594827867003	32.66745590444814	29.251742176270866	18.421319132580695
38	19.62649588492842	33.44575727444096	24.137232618064385	22.790514222566237
39	20.311225792085395	32.67290436937276	26.432576134894514	20.583293703647332
40	25.6694357107789	25.661855237840296	25.61098315772891	23.057725893651888
41	17.451196263774403	23.505566021477613	27.614300799088447	31.428936915659538
42	23.10279417416966	24.076825724334917	23.939725764546957	28.88065433694847
43	23.408381989506967	24.934840505072696	25.729605714729036	25.9271717906913
44	18.681601777620905	31.074431360890326	28.671066104685146	21.572900756803623
45	16.008537507647098	40.345290542353226	21.63431443209525	22.011857517904428
46	22.559369020383773	34.99815522084346	24.54669660163845	17.89577915713432
47	21.60488087701334	28.009965953016465	26.054559269526678	24.330593900443517
48	24.124144457756334	23.32067354870957	31.519369588919243	21.035812404614852
49	21.44989573888587	22.825159352793435	33.07437332289439	22.650571585426306
50	18.532775773756047	33.99919102140358	26.902920791756706	20.565112413083657
51	18.00338633939554	34.777137056727405	23.807067488121458	23.412409115755597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2362.0
1	2696.0
2	3030.0
3	7947.5
4	12865.0
5	9682.0
6	6499.0
7	7291.5
8	8084.0
9	9062.0
10	10040.0
11	10585.0
12	11130.0
13	10908.0
14	10686.0
15	10331.5
16	9977.0
17	9528.5
18	9080.0
19	8891.5
20	8703.0
21	8325.0
22	7947.0
23	7773.5
24	7600.0
25	7389.0
26	8954.5
27	10731.0
28	13381.5
29	16032.0
30	16771.0
31	17510.0
32	19611.5
33	21713.0
34	29635.5
35	37558.0
36	37388.5
37	37219.0
38	53063.0
39	68907.0
40	99433.5
41	129960.0
42	155874.5
43	181789.0
44	181784.5
45	181780.0
46	176355.5
47	170931.0
48	162959.5
49	154988.0
50	151573.0
51	148158.0
52	128610.0
53	109062.0
54	91385.0
55	73708.0
56	62715.5
57	51723.0
58	45442.5
59	39162.0
60	36664.0
61	34166.0
62	31055.0
63	27944.0
64	24724.5
65	21505.0
66	17426.0
67	13347.0
68	10995.5
69	8644.0
70	7873.5
71	7103.0
72	5660.5
73	4218.0
74	3431.5
75	2123.0
76	1601.0
77	1133.5
78	666.0
79	515.0
80	364.0
81	257.5
82	151.0
83	103.0
84	55.0
85	34.0
86	13.0
87	10.5
88	8.0
89	6.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1688549.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.154778081015994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.42412638013323	17.225093240123186
2	7.595077860012148	3.2134437327318963
3	2.5749332091468022	1.6341642183881682
4	1.2676011166059844	1.0726328126819071
5	0.7841818533024298	0.8294596540886372
6	0.5588326235750792	0.7093188081697648
7	0.38400946835231947	0.5686544558801576
8	0.3188666801965488	0.539644308559063
9	0.26644851516214485	0.5072993287444258
>10	2.7136547502899195	13.121894616608913
>50	0.9850673134978973	15.20044737723549
>100	1.113381662986358	42.2386950252281
>500	0.010434427945876111	1.4257288933950611
>1k	0.002820115661047598	0.6963985845091869
>5k	5.640231322095196E-4	1.0171249436560548
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9244	0.5474522800345148	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7805	0.46223118192009827	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1835	0.10867318626821015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.9222444832812075E-5	0.0	0.0	0.07349505403751978	0.0
2	5.9222444832812075E-5	0.0	0.0	0.2579137472468966	0.0
3	5.9222444832812075E-5	0.0	0.0	0.38364299762695664	0.0
4	1.1844488966562415E-4	0.0	0.0	0.6969889532373653	0.0
5	1.1844488966562415E-4	0.0	0.0	1.1580356862608074	0.0
6	1.1844488966562415E-4	0.0	0.0	1.931540038222166	0.0
7	1.1844488966562415E-4	0.0	0.0	2.317255821418271	0.0
8	1.1844488966562415E-4	0.0	0.0	3.326050946700392	0.0
9	1.1844488966562415E-4	0.0	0.0	3.844602673656494	0.0
10	1.1844488966562415E-4	0.0	0.0	4.692194304103701	0.0
11	1.1844488966562415E-4	0.0	0.0	5.258656988929548	0.0
12	1.1844488966562415E-4	0.0	0.0	5.807826719864215	0.0
13	1.1844488966562415E-4	0.0	0.0	6.045782503202454	0.0
14	1.1844488966562415E-4	0.0	0.0	6.14426942895942	0.0
15	1.1844488966562415E-4	0.0	0.0	6.249803825651491	0.0
16	1.1844488966562415E-4	0.0	0.0	6.449679576962232	0.0
17	1.1844488966562415E-4	0.0	0.0	6.656958133877074	0.0
18	1.1844488966562415E-4	0.0	0.0	7.005541444163006	0.0
19	1.1844488966562415E-4	0.0	0.0	7.13470559634337	0.0
20	1.1844488966562415E-4	0.0	0.0	7.281399592194245	0.0
21	1.1844488966562415E-4	0.0	0.0	7.459066926692681	0.0
22	1.1844488966562415E-4	0.0	0.0	7.636615816301452	0.0
23	1.1844488966562415E-4	0.0	0.0	7.841407030533316	0.0
24	1.1844488966562415E-4	0.0	0.0	7.993312601529479	0.0
25	1.1844488966562415E-4	0.0	0.0	8.110099262739784	0.0
26	1.1844488966562415E-4	0.0	0.0	8.23239361131954	0.0
27	1.1844488966562415E-4	0.0	0.0	8.358892753482428	0.0
28	1.1844488966562415E-4	0.0	0.0	8.474080408682248	0.0
29	1.1844488966562415E-4	0.0	0.0	8.609107582901059	0.0
30	1.1844488966562415E-4	0.0	0.0	8.841318789090515	0.0
31	1.1844488966562415E-4	0.0	0.0	9.00044949835628	0.0
32	1.1844488966562415E-4	0.0	0.0	9.131804880995459	0.0
33	1.1844488966562415E-4	0.0	0.0	9.26428549008646	0.0
34	1.1844488966562415E-4	0.0	0.0	9.403872792557397	0.0
35	1.1844488966562415E-4	0.0	0.0	9.598418523833185	0.0
36	1.1844488966562415E-4	0.0	0.0	9.746829970584212	0.0
37	1.1844488966562415E-4	0.0	0.0	9.893346299100589	0.0
38	1.7766733449843622E-4	0.0	0.0	10.039862627616966	0.0
39	1.7766733449843622E-4	0.0	0.0	10.182470274774378	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATCG	20	7.034227E-4	45.000004	17
CCCTATA	20	7.034227E-4	45.000004	44
ACGTTAC	30	2.166109E-6	45.000004	39
GTTTACG	20	7.034227E-4	45.000004	1
TACGGAC	20	7.034227E-4	45.000004	41
TCGATAG	35	1.2123746E-7	45.000004	1
CGTTATG	20	7.034227E-4	45.000004	13
ATCGTAG	40	6.8175723E-9	45.000004	1
TACGATT	20	7.034227E-4	45.000004	23
ACTACGG	35	1.2123746E-7	45.000004	2
ACTAACG	40	6.8175723E-9	45.000004	1
ACGCTCG	20	7.034227E-4	45.000004	42
CCGGTTA	20	7.034227E-4	45.000004	21
ATAACCG	20	7.034227E-4	45.000004	1
ATACGTT	20	7.034227E-4	45.000004	30
ATCGCGA	20	7.034227E-4	45.000004	20
TCCGCTA	25	3.8915856E-5	45.0	23
TGTATCG	25	3.8915856E-5	45.0	13
CACTAGT	25	3.8915856E-5	45.0	36
ACGGATA	25	3.8915856E-5	45.0	34
>>END_MODULE