FastQCFastQC Report
Thu 26 May 2016
SRR1546685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546685_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1847298
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121530.6578797790069604No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26500.14345276181753025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA302.16622E-645.00000440
TCACGAC850.042.35294325
TATAGCG700.041.7857131
TTTACGG1500.040.5000042
CGTTTTT71800.040.236771
CGAATAT1450.038.79310614
GGGCGAT38400.038.2617237
ACGTTAG950.037.8947371
GTATACG950.037.8947371
AGGGCGA20700.037.8260886
TTACGGG4550.037.5824173
TACGGGA5050.037.4257434
AGGGTAC7700.037.4025966
CGTTAGG2650.037.358492
TGGGCGA10750.037.2558146
CGTAAGG1950.036.923082
TAGGGAC15000.036.65
TCGATAG800.036.56251
CTACGGG3150.036.4285743
ACGGGAT6800.036.397065
Produced by FastQC (version 0.11.5)