##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546685_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1847298
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42784867411755	31.0	31.0	33.0	30.0	34.0
2	31.838405065127553	31.0	31.0	34.0	30.0	34.0
3	31.959489481393906	33.0	31.0	34.0	30.0	34.0
4	35.72685457354471	37.0	35.0	37.0	33.0	37.0
5	35.64709321398063	37.0	35.0	37.0	33.0	37.0
6	35.715361571332835	37.0	35.0	37.0	33.0	37.0
7	36.013470485000255	37.0	35.0	37.0	35.0	37.0
8	35.97951061496304	37.0	35.0	37.0	35.0	37.0
9	37.71608966176545	39.0	37.0	39.0	35.0	39.0
10	37.20224241026624	39.0	37.0	39.0	33.0	39.0
11	36.99551398853894	39.0	37.0	39.0	33.0	39.0
12	36.59834038687857	39.0	35.0	39.0	32.0	39.0
13	36.51266011222878	39.0	35.0	39.0	32.0	39.0
14	37.60931533515437	40.0	36.0	41.0	33.0	41.0
15	37.73170381822532	40.0	36.0	41.0	33.0	41.0
16	37.76814244372051	40.0	36.0	41.0	33.0	41.0
17	37.68639223341334	40.0	35.0	41.0	33.0	41.0
18	37.57262932131145	39.0	36.0	41.0	33.0	41.0
19	37.526820794479285	39.0	36.0	41.0	33.0	41.0
20	37.39006429931717	39.0	35.0	41.0	32.0	41.0
21	37.294066252440054	39.0	35.0	41.0	32.0	41.0
22	37.19385935566433	39.0	35.0	41.0	32.0	41.0
23	37.10499713635807	39.0	35.0	41.0	32.0	41.0
24	36.99601905052677	39.0	35.0	41.0	32.0	41.0
25	36.89242504457862	39.0	35.0	41.0	31.0	41.0
26	36.764319021619684	38.0	35.0	41.0	31.0	41.0
27	36.66441581163407	38.0	35.0	41.0	31.0	41.0
28	36.5725562416026	38.0	35.0	40.0	31.0	41.0
29	36.586134451507014	38.0	35.0	41.0	31.0	41.0
30	36.573884126978975	38.0	35.0	40.0	31.0	41.0
31	36.503577116415435	38.0	35.0	41.0	31.0	41.0
32	36.41028572542167	38.0	35.0	41.0	30.0	41.0
33	36.28081175857929	38.0	35.0	41.0	30.0	41.0
34	36.11825758486178	38.0	35.0	41.0	30.0	41.0
35	36.0097336758877	38.0	35.0	41.0	30.0	41.0
36	35.90386066568578	38.0	35.0	41.0	29.0	41.0
37	35.85406415207508	38.0	35.0	41.0	29.0	41.0
38	35.772028660237815	38.0	35.0	41.0	29.0	41.0
39	35.675631110952324	38.0	35.0	40.0	28.0	41.0
40	35.55788887337073	38.0	35.0	40.0	28.0	41.0
41	35.49467438388392	38.0	35.0	40.0	28.0	41.0
42	35.42301025606047	38.0	35.0	40.0	27.0	41.0
43	35.30619423612217	38.0	34.0	40.0	27.0	41.0
44	35.16423608968342	38.0	34.0	40.0	27.0	41.0
45	35.05206414990976	37.0	34.0	40.0	26.0	41.0
46	35.01721974472987	37.0	34.0	40.0	26.0	41.0
47	34.9636128009666	37.0	34.0	40.0	26.0	41.0
48	34.86663386199736	37.0	34.0	40.0	26.0	41.0
49	34.7713260123705	37.0	34.0	40.0	26.0	41.0
50	34.67827010043859	36.0	34.0	40.0	26.0	41.0
51	34.309270079868	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	5.0
12	6.0
13	9.0
14	26.0
15	55.0
16	123.0
17	308.0
18	651.0
19	1313.0
20	2248.0
21	3746.0
22	5711.0
23	8449.0
24	12302.0
25	18082.0
26	24339.0
27	28861.0
28	30890.0
29	34954.0
30	40972.0
31	50449.0
32	63050.0
33	82206.0
34	134143.0
35	201009.0
36	146375.0
37	186475.0
38	275620.0
39	494625.0
40	292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.13829495836622	23.1174937665715	26.22154086671452	14.52267040834776
2	33.01080821827339	25.158258169499454	28.303067507245714	13.527866104981436
3	29.053027719404234	25.903454667303272	31.010048189301347	14.033469423991148
4	25.916717281131685	27.609026805637203	30.81814628717186	15.65610962605925
5	22.66061025346208	31.86118319837947	29.95071720967597	15.527489338482475
6	21.230900482759143	41.151184053682734	27.1595053965305	10.458410067027627
7	86.37642654298332	4.035136724015292	7.618099516158194	1.970337216843195
8	86.1883680922082	3.877230419780674	7.353713369472603	2.5806881185385357
9	80.35990944612077	5.909008725175906	9.865814827927059	3.8652670007762686
10	41.95868776992126	26.855277275242003	16.811256223955205	14.374778730881536
11	33.6481174125669	25.478617959852716	24.63544051907164	16.237824108508754
12	29.54260763558451	22.885262691780103	29.35368305492671	18.218446617708675
13	23.448571914222825	28.78398612459928	29.728500761652967	18.038941199524928
14	19.994879007068704	31.130710908581072	30.4210257359668	18.453384348383423
15	19.183964904417152	24.31443113130637	38.440305787155076	18.061298177121397
16	21.854243332694562	22.460425984329547	35.999768310256385	19.685562372719506
17	21.402069400822175	23.37348928001871	29.73164048247765	25.492800836681468
18	23.61140433216514	23.390432945848477	32.16194680013728	20.8362159218491
19	27.05784340155189	25.82945469545249	26.87162547677743	20.241076426218186
20	28.87806948310451	24.14001422618332	26.776892520860194	20.20502376985197
21	25.501732801096523	26.28612167609124	28.194205807617394	20.017939715194842
22	24.207680623267063	24.27004197481944	27.784418106878263	23.737859295035236
23	22.512123111701523	27.208712400489798	27.438615751221512	22.840548736587166
24	22.58163003478594	24.396659337042532	32.410093011522775	20.611617616648747
25	21.637277797085254	26.160641109339156	30.2983600913334	21.903721002242193
26	21.02811782397859	28.713992003455857	28.36331766720908	21.89457250535647
27	20.971819381604917	27.697750985493407	29.03559685551546	22.294832777386215
28	20.020321572372186	27.212880650550154	32.105919023351944	20.66087875372571
29	21.10926336736141	25.10374612000879	31.876611136914562	21.910379375715234
30	22.32844944345742	26.487984071871455	30.745716175733424	20.4378503089377
31	25.91124983624732	25.830050159746833	27.601339902928494	20.65736010107736
32	25.975018648859038	25.728929495944886	28.085939572283408	20.210112282912664
33	25.01150328750424	26.047394627179806	27.120908483633933	21.820193601682025
34	21.618222939666477	26.95715580269128	29.35817610369307	22.066445153949175
35	21.930841694193358	27.00132842670755	29.443219231547914	21.62461064755118
36	24.82858748290747	27.572216285623654	27.052375956667525	20.54682027480136
37	22.79513105086456	28.990720500969523	27.650438640652457	20.56370980751346
38	22.34853283011187	29.819931597392518	26.456586863624604	21.37494870887101
39	22.16226077221975	29.317413866089826	26.428383509320096	22.09194185237033
40	23.567989571796215	26.491123792696143	27.58320530851005	22.357681326997593
41	20.332886193781405	26.326288449400153	28.037598698206782	25.30322665861166
42	22.719940150425106	26.952987552630926	26.817275826639776	23.509796470304195
43	22.798812102865913	25.76812187313579	27.937560696758183	23.49550532724011
44	21.822629591977037	27.594464996984787	28.628894742483347	21.95401066855483
45	20.436334581643028	29.502549128510942	27.192364198954365	22.868752090891668
46	22.709167660009374	28.250395983755734	27.33592522700723	21.70451112922766
47	21.674792047628483	27.37766186072848	28.54866946210086	22.398876629542176
48	22.711387117833723	26.0139403604616	29.530914882168442	21.74375763953623
49	22.31561989457034	25.556028318116514	29.728717294123637	22.399634493189513
50	21.214335748753044	28.545150809452508	28.73104393552096	21.509469506273486
51	21.18255960868252	28.669440447615923	27.422429949039085	22.725569994662475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1557.0
1	2329.0
2	3101.0
3	11907.0
4	20713.0
5	14189.5
6	7666.0
7	7830.5
8	7995.0
9	8416.0
10	8837.0
11	9042.5
12	9248.0
13	9453.0
14	9658.0
15	9380.5
16	9103.0
17	8751.0
18	8399.0
19	8373.0
20	8347.0
21	8777.5
22	9208.0
23	9768.5
24	10329.0
25	11764.5
26	15575.5
27	17951.0
28	20819.0
29	23687.0
30	27153.0
31	30619.0
32	35277.5
33	39936.0
34	45962.5
35	51989.0
36	55798.5
37	59608.0
38	65908.0
39	72208.0
40	86656.0
41	101104.0
42	114100.0
43	127096.0
44	135398.5
45	143701.0
46	144342.5
47	144984.0
48	141599.5
49	138215.0
50	131050.0
51	123885.0
52	119125.0
53	114365.0
54	105671.0
55	96977.0
56	91937.5
57	86898.0
58	83752.5
59	80607.0
60	74788.5
61	68970.0
62	61848.5
63	54727.0
64	48769.0
65	42811.0
66	37556.5
67	32302.0
68	27947.5
69	23593.0
70	20279.5
71	16966.0
72	13795.5
73	10625.0
74	9289.0
75	5915.5
76	3878.0
77	3070.0
78	2262.0
79	1756.0
80	1250.0
81	838.0
82	426.0
83	315.5
84	205.0
85	138.5
86	72.0
87	58.5
88	45.0
89	28.0
90	11.0
91	8.5
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1847298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.841598128031897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44990245635267	17.690795469632782
2	6.1089629921295385	2.790769552904844
3	2.4512634283965973	1.6797232241213031
4	1.4489849096652165	1.323885240006219
5	1.0293729435610501	1.1756261550345382
6	0.85837951020071	1.1764055888004883
7	0.6908608181411617	1.1046255619268621
8	0.5505394020267779	1.006015981979412
9	0.49921958623300977	1.0262675849739096
>10	7.110141002623285	39.00859274688786
>50	1.60136701164369	25.068619812749564
>100	0.19837058142067399	5.799043142247785
>500	0.0019166237131740533	0.2801839911614592
>1k	4.7915592829351334E-4	0.2043920326148535
>5k	0.0	0.0
>10k+	2.3957796414675667E-4	0.6650539149580496
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12153	0.6578797790069604	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2650	0.14345276181753025	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.413311766699255E-5	0.0	0.0	0.03128894201152169	0.0
2	5.413311766699255E-5	0.0	0.0	0.11432914451268826	0.0
3	5.413311766699255E-5	0.0	0.0	0.18784191830446414	0.0
4	5.413311766699255E-5	0.0	0.0	0.3240949754722844	0.0
5	5.413311766699255E-5	0.0	0.0	0.5780334304481465	0.0
6	5.413311766699255E-5	0.0	0.0	1.0586272490957063	0.0
7	5.413311766699255E-5	0.0	0.0	1.3026593435385088	0.0
8	5.413311766699255E-5	0.0	0.0	1.9427834599506955	0.0
9	5.413311766699255E-5	0.0	0.0	2.2999537703175124	0.0
10	5.413311766699255E-5	0.0	0.0	2.824341281157669	0.0
11	5.413311766699255E-5	0.0	0.0	3.1356608408605435	0.0
12	5.413311766699255E-5	0.0	0.0	3.428196208732971	0.0
13	5.413311766699255E-5	0.0	0.0	3.57419322708085	0.0
14	5.413311766699255E-5	0.0	0.0	3.647760133990293	0.0
15	5.413311766699255E-5	0.0	0.0	3.7177542551337144	0.0
16	5.413311766699255E-5	0.0	0.0	3.834032191882414	0.0
17	5.413311766699255E-5	0.0	0.0	3.953612248808801	0.0
18	5.413311766699255E-5	0.0	0.0	4.146975745115298	0.0
19	5.413311766699255E-5	0.0	0.0	4.243332694562545	0.0
20	5.413311766699255E-5	0.0	0.0	4.342396299893141	0.0
21	5.413311766699255E-5	0.0	0.0	4.464574746467544	0.0
22	5.413311766699255E-5	0.0	0.0	4.587186257983282	0.0
23	5.413311766699255E-5	0.0	0.0	4.73372460750783	0.0
24	5.413311766699255E-5	0.0	0.0	4.849731878668195	0.0
25	5.413311766699255E-5	0.0	0.0	4.94847068529279	0.0
26	5.413311766699255E-5	0.0	0.0	5.045044167210705	0.0
27	5.413311766699255E-5	0.0	0.0	5.1548802629570325	0.0
28	5.413311766699255E-5	0.0	0.0	5.259519579407329	0.0
29	1.082662353339851E-4	0.0	0.0	5.382455889629069	0.0
30	1.082662353339851E-4	0.0	0.0	5.556331463575449	0.0
31	1.082662353339851E-4	0.0	0.0	5.678564043267518	0.0
32	1.082662353339851E-4	0.0	0.0	5.811027782198649	0.0
33	1.082662353339851E-4	0.0	0.0	5.941705128246769	0.0
34	1.082662353339851E-4	0.0	0.0	6.073411003530562	0.0
35	1.082662353339851E-4	0.0	0.0	6.219516288113775	0.0
36	1.082662353339851E-4	0.0	0.0	6.358151202458942	0.0
37	1.082662353339851E-4	0.0	0.0	6.499763438275795	0.0
38	1.082662353339851E-4	0.0	0.0	6.651065502155039	0.0
39	1.082662353339851E-4	0.0	0.0	6.835875965870152	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACA	30	2.16622E-6	45.000004	40
TCACGAC	85	0.0	42.352943	25
TATAGCG	70	0.0	41.785713	1
TTTACGG	150	0.0	40.500004	2
CGTTTTT	7180	0.0	40.23677	1
CGAATAT	145	0.0	38.793106	14
GGGCGAT	3840	0.0	38.261723	7
ACGTTAG	95	0.0	37.894737	1
GTATACG	95	0.0	37.894737	1
AGGGCGA	2070	0.0	37.826088	6
TTACGGG	455	0.0	37.582417	3
TACGGGA	505	0.0	37.425743	4
AGGGTAC	770	0.0	37.402596	6
CGTTAGG	265	0.0	37.35849	2
TGGGCGA	1075	0.0	37.255814	6
CGTAAGG	195	0.0	36.92308	2
TAGGGAC	1500	0.0	36.6	5
TCGATAG	80	0.0	36.5625	1
CTACGGG	315	0.0	36.428574	3
ACGGGAT	680	0.0	36.39706	5
>>END_MODULE