FastQCFastQC Report
Thu 26 May 2016
SRR1546684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546684_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1220173
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227771.8667025085787017No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27140.22242747544815367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG302.1656124E-645.0000041
TCTTGCG253.8909475E-545.01
CCCTCGA207.033458E-445.042
TCCGCAC253.8909475E-545.044
CGCATCG551.8189894E-1245.021
TAGCGGA207.033458E-445.03
GCGCCTA253.8909475E-545.09
CCTCGCG207.033458E-445.01
GAGCCGT207.033458E-445.012
CCGCATC551.8189894E-1245.020
ATCGAGT207.033458E-445.029
TACCGGT207.033458E-445.040
CGTTTTT116950.044.0957641
CGGGACG700.041.7857176
GCGATAC700.041.7857179
ATAACGG700.041.7857172
TATTGCG650.041.538461
TACGCGG650.041.538462
GCGGGTG1300.041.538465
TCGTAAG556.184564E-1140.9090921
Produced by FastQC (version 0.11.5)