##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546684_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1220173
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334655823395536	31.0	31.0	33.0	30.0	34.0
2	31.732537107442962	31.0	31.0	34.0	30.0	34.0
3	31.66540072596263	31.0	31.0	34.0	30.0	34.0
4	35.5222661048884	37.0	35.0	37.0	33.0	37.0
5	35.47362136352796	37.0	35.0	37.0	33.0	37.0
6	35.554223868254745	37.0	35.0	37.0	33.0	37.0
7	36.007789878976176	37.0	35.0	37.0	35.0	37.0
8	36.00753663619831	37.0	35.0	37.0	35.0	37.0
9	37.73541866604162	39.0	38.0	39.0	35.0	39.0
10	37.135734850713796	39.0	37.0	39.0	33.0	39.0
11	36.83890972837458	39.0	37.0	39.0	32.0	39.0
12	36.325681686121555	38.0	35.0	39.0	32.0	39.0
13	36.1437337164484	38.0	35.0	39.0	32.0	39.0
14	37.21290669437858	39.0	35.0	41.0	32.0	41.0
15	37.439661425060216	39.0	35.0	41.0	32.0	41.0
16	37.50769931804752	39.0	35.0	41.0	33.0	41.0
17	37.45096801846951	39.0	35.0	41.0	32.0	41.0
18	37.26533778406832	39.0	35.0	41.0	32.0	41.0
19	37.11095557761072	38.0	35.0	41.0	32.0	41.0
20	36.86884646685347	38.0	35.0	41.0	32.0	41.0
21	36.76812960129424	38.0	35.0	41.0	32.0	41.0
22	36.6657867367988	38.0	35.0	40.0	32.0	41.0
23	36.603173484415734	38.0	35.0	40.0	32.0	41.0
24	36.46308351356734	38.0	35.0	40.0	31.0	41.0
25	36.36394675181306	38.0	35.0	40.0	31.0	41.0
26	36.221421060784	37.0	35.0	40.0	31.0	41.0
27	36.09981043671676	37.0	35.0	40.0	31.0	41.0
28	36.032535550286724	37.0	35.0	40.0	31.0	41.0
29	35.995284275262605	37.0	35.0	40.0	30.0	41.0
30	35.92639240501142	37.0	35.0	40.0	30.0	41.0
31	35.754647086929474	37.0	35.0	40.0	30.0	41.0
32	35.45646641910614	37.0	34.0	40.0	29.0	41.0
33	35.13360810311325	37.0	34.0	40.0	27.0	41.0
34	34.83421531209099	37.0	34.0	40.0	24.0	41.0
35	34.586783185663016	37.0	34.0	40.0	23.0	41.0
36	34.3889841850295	37.0	34.0	40.0	22.0	41.0
37	34.30018612114839	37.0	34.0	40.0	22.0	41.0
38	34.237044255199876	37.0	33.0	40.0	22.0	41.0
39	34.15319712860389	36.0	33.0	40.0	21.0	41.0
40	34.0357900068269	36.0	33.0	40.0	21.0	41.0
41	34.00203495733802	36.0	33.0	40.0	21.0	41.0
42	33.93465762641855	36.0	33.0	40.0	20.0	41.0
43	33.822094080101756	36.0	33.0	40.0	20.0	41.0
44	33.67492806347952	36.0	33.0	40.0	18.0	41.0
45	33.567185964613216	35.0	33.0	40.0	19.0	41.0
46	33.55048833239221	35.0	33.0	40.0	19.0	41.0
47	33.526110641687694	35.0	33.0	40.0	20.0	41.0
48	33.432079713286555	35.0	33.0	40.0	18.0	41.0
49	33.371796458371065	36.0	33.0	40.0	18.0	41.0
50	33.26640894364979	35.0	33.0	40.0	18.0	41.0
51	32.9055568349734	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	4.0
13	0.0
14	6.0
15	31.0
16	86.0
17	238.0
18	583.0
19	1210.0
20	2036.0
21	3460.0
22	5507.0
23	8235.0
24	13028.0
25	20708.0
26	28054.0
27	29942.0
28	28133.0
29	27873.0
30	31009.0
31	36598.0
32	44730.0
33	57858.0
34	94753.0
35	162407.0
36	99549.0
37	108612.0
38	148289.0
39	267025.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.078335613064706	24.279671817029225	27.988654067906765	18.653338501999308
2	35.80992203564576	24.98670270527212	26.748256189900943	12.455119069181174
3	26.001640750942695	24.216729922724074	36.696107847002025	13.085521479331211
4	22.25299199375826	27.259577125538755	36.16077392304206	14.326656957660921
5	21.061849426269884	30.8947173884359	35.09584296653016	12.94759021876406
6	19.29537860614847	38.87293031398006	31.66370670388543	10.167984375986027
7	82.36061607657274	3.6755443695279277	12.415288651691196	1.5485509022081294
8	83.03248801604363	2.858856899800274	11.908967007137512	2.1996880770185867
9	77.70111287497757	5.311459932321073	14.237489274061957	2.749937918639406
10	41.54509237624501	28.79140908707208	19.302754609387357	10.360743927295555
11	33.73374103508273	23.399632675038703	29.48114734549937	13.385478944379198
12	30.584679385628107	22.874707111204724	31.107146281715792	15.433467221451385
13	19.995115446744027	34.08434705570439	32.40704391918195	13.513493578369626
14	15.922824058555632	37.38814086199252	32.69823213593482	13.990802943517025
15	14.35714443771498	26.279388250682484	46.110510558748636	13.252956752853898
16	16.01035263032373	23.389961915236608	44.17660446510454	16.42308098933512
17	16.761803449183027	23.934720732224037	33.247744377231754	26.055731441361186
18	19.428392531223032	26.37322740299941	36.64644275852687	17.551937307250693
19	23.99610547028987	28.313607988375416	30.032298698627162	17.657987842707552
20	25.446883351787005	26.59188492123658	29.45631480126179	18.50491692571463
21	20.003802739447604	29.892646370637603	32.374917327297034	17.72863356261776
22	20.333673995408848	26.817672575938	30.36233386577149	22.486319562881658
23	17.395647994177875	32.14765447194783	29.08235143705032	21.374346096823977
24	17.930408228997035	27.97578704003449	37.75972751404923	16.33407721691924
25	16.658047670289378	29.418451317968845	35.825739464813594	18.097761546928183
26	16.31252289634339	34.49010918943461	31.06452937411334	18.132838540108658
27	16.721809120509963	34.171301938331695	31.8554008325049	17.251488108653447
28	15.839966955505492	30.781290849740163	37.71055415912334	15.668188035631015
29	15.78612213186163	29.077024323600014	36.305589453298836	18.83126409123952
30	18.192993944301342	32.17052008198837	32.879435948836765	16.75705002487352
31	24.152148916588057	30.3688083575034	28.432197729338384	17.046844996570158
32	24.455220694114686	30.909305483730588	28.692406732487935	15.943067089666792
33	23.515272014706113	31.52954540052927	27.546093873573664	17.409088711190954
34	19.06205103702508	31.793278494115178	29.054076757967927	20.090593710891817
35	19.569028326311106	30.7580154617419	30.158756176378265	19.514200035568727
36	25.07980425726516	30.190309079122386	27.47659553194506	17.253291131667396
37	20.41481003103658	33.10210929105955	29.863879958005956	16.61920071989792
38	19.658605787867785	34.42413493824236	26.51533839873526	19.401920875154588
39	19.76408263418384	33.470089897088364	28.09568807046214	18.670139398265658
40	21.669140359604743	30.08696307818645	27.519950039871393	20.723946522337407
41	17.61553484628819	28.813782963563362	28.981136281494507	24.589545908653935
42	20.09977273714465	30.627542160005177	26.703016703369116	22.569668399481056
43	20.750746000772022	30.23136883048551	28.29836424834839	20.719520920394075
44	19.52829639731415	33.51311658264853	28.090524868194922	18.868062151842402
45	17.724453827449057	36.550308849646726	25.532117166991892	20.193120155912318
46	21.357053467008367	34.22244222745463	26.1478495262557	18.2726547792813
47	20.608225227078456	31.501680499404593	27.165901884404914	20.724192389112037
48	20.988908949796464	29.72603065303035	29.377063744239546	19.90799665293364
49	20.058385163415352	28.433427063211532	30.15580577508271	21.352381998290408
50	18.997306119705975	33.189064173686845	28.36794454556854	19.44568516103864
51	18.027197782609516	34.24563565986134	26.38666812001249	21.340498437516647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1506.0
1	1574.5
2	1643.0
3	15627.5
4	29612.0
5	19633.0
6	9654.0
7	10508.0
8	11362.0
9	13312.5
10	15263.0
11	16849.5
12	18436.0
13	18943.0
14	19450.0
15	18895.5
16	18341.0
17	17041.5
18	15742.0
19	14447.5
20	13153.0
21	12085.5
22	11018.0
23	10906.0
24	10794.0
25	10991.0
26	11965.5
27	12743.0
28	14457.5
29	16172.0
30	18213.5
31	20255.0
32	22671.0
33	25087.0
34	27634.5
35	30182.0
36	32933.0
37	35684.0
38	42550.5
39	49417.0
40	61907.0
41	74397.0
42	86416.0
43	98435.0
44	105264.0
45	112093.0
46	114547.5
47	117002.0
48	113590.0
49	110178.0
50	101755.5
51	93333.0
52	82936.5
53	72540.0
54	61441.0
55	50342.0
56	43632.5
57	36923.0
58	32069.5
59	27216.0
60	22446.5
61	17677.0
62	15078.5
63	12480.0
64	10716.5
65	8953.0
66	6955.5
67	4958.0
68	3899.0
69	2840.0
70	2159.5
71	1479.0
72	1329.0
73	1179.0
74	919.5
75	471.0
76	282.0
77	291.0
78	300.0
79	206.5
80	113.0
81	80.5
82	48.0
83	34.5
84	21.0
85	16.0
86	11.0
87	8.5
88	6.0
89	4.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1220173.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.31255409318715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.61007213381056	24.75467099906723
2	8.08356845523812	5.22401485951726
3	3.5964515723568633	3.486316079259274
4	2.097102130821461	2.7105090416442597
5	1.342663434871296	2.1692442434111605
6	1.0093301487137325	1.9568421016918272
7	0.7796681608834909	1.7635148736298476
8	0.6186328296164403	1.5991685416642125
9	0.5006242914987301	1.4558804549474587
>10	4.522269868789347	31.091488130274247
>50	0.715194107131602	15.796034650764812
>100	0.122128182265943	5.407548084859144
>500	0.001274824445778559	0.2999662161942766
>1k	7.648946674671354E-4	0.4083033692553789
>5k	0.0	0.0
>10k+	2.5496488915571183E-4	1.8764983538195654
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22777	1.8667025085787017	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2714	0.22242747544815367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050894422348306344	0.0
2	0.0	0.0	0.0	0.20693786864649522	0.0
3	0.0	0.0	0.0	0.3716686076482597	0.0
4	0.0	0.0	0.0	0.5976201735327695	0.0
5	0.0	0.0	0.0	1.038131478077289	0.0
6	0.0	0.0	0.0	1.92153079932108	0.0
7	0.0	0.0	0.0	2.4415390276624707	0.0
8	0.0	0.0	0.0	3.520238523553627	0.0
9	0.0	0.0	0.0	4.131545280874105	0.0
10	0.0	0.0	0.0	4.932415321433928	0.0
11	0.0	0.0	0.0	5.468159023351607	0.0
12	0.0	0.0	0.0	5.9506315907662275	0.0
13	0.0	0.0	0.0	6.220839176084047	0.0
14	0.0	0.0	0.0	6.35368918997552	0.0
15	0.0	0.0	0.0	6.468836796093669	0.0
16	0.0	0.0	0.0	6.6652023934310956	0.0
17	0.0	0.0	0.0	6.879516265316475	0.0
18	0.0	0.0	0.0	7.228647085290365	0.0
19	0.0	0.0	0.0	7.376904750391953	0.0
20	0.0	0.0	0.0	7.548028025534084	0.0
21	0.0	0.0	0.0	7.735788285759479	0.0
22	0.0	0.0	0.0	7.926253080505797	0.0
23	0.0	0.0	0.0	8.16834989792431	0.0
24	0.0	0.0	0.0	8.348242421361562	0.0
25	0.0	0.0	0.0	8.505597157124441	0.0
26	0.0	0.0	0.0	8.652543532761339	0.0
27	0.0	0.0	0.0	8.816372760256128	0.0
28	0.0	0.0	0.0	8.987905813355974	0.0
29	0.0	0.0	0.0	9.168535937117113	0.0
30	0.0	0.0	0.0	9.448332326645485	0.0
31	0.0	0.0	0.0	9.646009213447602	0.0
32	0.0	0.0	0.0	9.830901027968984	0.0
33	0.0	0.0	0.0	10.01841542141975	0.0
34	0.0	0.0	0.0	10.215600574672608	0.0
35	0.0	0.0	0.0	10.446633387232794	0.0
36	0.0	0.0	0.0	10.648571964795156	0.0
37	0.0	0.0	0.0	10.849117297301284	0.0
38	0.0	0.0	0.0	11.050564141314387	0.0
39	0.0	0.0	0.0	11.276679618381984	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	30	2.1656124E-6	45.000004	1
TCTTGCG	25	3.8909475E-5	45.0	1
CCCTCGA	20	7.033458E-4	45.0	42
TCCGCAC	25	3.8909475E-5	45.0	44
CGCATCG	55	1.8189894E-12	45.0	21
TAGCGGA	20	7.033458E-4	45.0	3
GCGCCTA	25	3.8909475E-5	45.0	9
CCTCGCG	20	7.033458E-4	45.0	1
GAGCCGT	20	7.033458E-4	45.0	12
CCGCATC	55	1.8189894E-12	45.0	20
ATCGAGT	20	7.033458E-4	45.0	29
TACCGGT	20	7.033458E-4	45.0	40
CGTTTTT	11695	0.0	44.095764	1
CGGGACG	70	0.0	41.785717	6
GCGATAC	70	0.0	41.785717	9
ATAACGG	70	0.0	41.785717	2
TATTGCG	65	0.0	41.53846	1
TACGCGG	65	0.0	41.53846	2
GCGGGTG	130	0.0	41.53846	5
TCGTAAG	55	6.184564E-11	40.909092	1
>>END_MODULE