FastQCFastQC Report
Thu 26 May 2016
SRR1546683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546683_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3716043
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT563581.516613236176223No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53460.14386270557149097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT330500.043.6996961
GCGAACG750.042.01
CGTTAGG4300.039.767442
ACGTTAG1650.039.5454521
CGACGGT1450.038.79310228
AGGGCGA34050.038.7224666
CTATTCG356.2520576E-638.57142627
AAGGGCG13650.038.0769235
GACCGAT23000.037.760879
TTACGGG9000.037.753
GGGCGAT73800.037.5914657
TACGGGA13050.037.586214
ACGGGAT16200.037.55
CGCTAGG3000.037.52
CCGCCTA301.14041264E-437.49999639
GGGACCG26200.037.2709967
GGACCGA24300.037.222228
TAGGGCG14900.037.147655
GTTTTTT395500.036.9500662
TAAGGGA50500.036.891094
Produced by FastQC (version 0.11.5)