FastQCFastQC Report
Thu 26 May 2016
SRR1546682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546682_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2591095
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122880.4742396554352503No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC102960.39736096129242654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC302.16656E-645.00000429
ACGTTAC453.8562575E-1045.039
CCTACGT207.034927E-445.029
GCGATCG351.2127202E-745.09
CGTAATC207.034927E-445.035
ACGTATA207.034927E-445.010
TACGTAA253.892165E-545.010
TACGTCA650.044.99999638
GTTACAC1350.043.33333233
GCGTAAG2700.043.3333321
CGAATAT2100.042.85714314
GTCAACG1450.041.896551
CGTTTTT82200.041.6879581
TAACGTT556.184564E-1140.90909236
TCGATTG1100.040.9090921
TATTACG501.0822987E-940.51
CGTACCG501.0822987E-940.534
CGACGGT3400.040.36764528
ATCGTAC451.9299478E-840.028
TACGCGC451.9299478E-840.034