##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546682_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2591095
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333430460866932	31.0	31.0	33.0	30.0	34.0
2	31.72800649918278	31.0	31.0	34.0	30.0	34.0
3	31.85894534936002	31.0	31.0	34.0	30.0	34.0
4	35.607724533450146	37.0	35.0	37.0	33.0	37.0
5	35.49845914565078	37.0	35.0	37.0	33.0	37.0
6	35.571773323633444	37.0	35.0	37.0	33.0	37.0
7	35.955030595173085	37.0	35.0	37.0	35.0	37.0
8	35.94317884909662	37.0	35.0	37.0	35.0	37.0
9	37.675419079578326	39.0	37.0	39.0	35.0	39.0
10	37.069819516459255	39.0	37.0	39.0	33.0	39.0
11	36.806613034257715	39.0	35.0	39.0	32.0	39.0
12	35.98085056703826	37.0	35.0	39.0	32.0	39.0
13	35.61069161879437	37.0	35.0	39.0	31.0	39.0
14	36.56768624847796	38.0	35.0	41.0	31.0	41.0
15	36.87251914731031	38.0	35.0	41.0	32.0	41.0
16	37.02753700655514	38.0	35.0	41.0	32.0	41.0
17	36.98035386583665	38.0	35.0	41.0	32.0	41.0
18	36.918772565266806	38.0	35.0	41.0	32.0	41.0
19	36.849944135587464	38.0	35.0	41.0	32.0	41.0
20	36.68763167695511	38.0	35.0	41.0	32.0	41.0
21	36.54270993537481	38.0	35.0	40.0	31.0	41.0
22	36.431040158697385	38.0	35.0	40.0	31.0	41.0
23	36.394178908916885	38.0	35.0	40.0	31.0	41.0
24	36.26899476862099	37.0	35.0	40.0	31.0	41.0
25	36.17105509446778	37.0	35.0	40.0	31.0	41.0
26	36.02033927740975	37.0	35.0	40.0	31.0	41.0
27	35.93184078545943	37.0	35.0	40.0	30.0	41.0
28	35.887125327323005	37.0	35.0	40.0	30.0	41.0
29	35.95516374351384	37.0	35.0	40.0	30.0	41.0
30	35.959991818130945	37.0	35.0	40.0	30.0	41.0
31	35.85514078024928	37.0	35.0	40.0	30.0	41.0
32	35.70042510984738	37.0	34.0	40.0	30.0	41.0
33	35.583888664830894	37.0	34.0	40.0	30.0	41.0
34	35.47048757378637	37.0	34.0	40.0	29.0	41.0
35	35.403088269631176	37.0	34.0	40.0	29.0	41.0
36	35.24825141494233	37.0	34.0	40.0	28.0	41.0
37	35.187501423143495	37.0	34.0	40.0	28.0	41.0
38	35.15291797483303	36.0	34.0	40.0	28.0	41.0
39	35.11077208670466	36.0	34.0	40.0	27.0	41.0
40	34.964149519797616	36.0	34.0	40.0	27.0	41.0
41	34.94121828802109	36.0	34.0	40.0	27.0	41.0
42	34.87481856126464	36.0	34.0	40.0	27.0	41.0
43	34.75621696618611	36.0	34.0	40.0	27.0	41.0
44	34.610707828157594	35.0	34.0	40.0	26.0	41.0
45	34.49146789291786	35.0	34.0	40.0	26.0	41.0
46	34.47504780797308	35.0	34.0	40.0	26.0	41.0
47	34.4460623790328	35.0	34.0	40.0	26.0	41.0
48	34.39860213539064	35.0	34.0	40.0	26.0	41.0
49	34.36406306986043	36.0	34.0	40.0	26.0	41.0
50	34.23173368788099	35.0	34.0	40.0	25.0	41.0
51	33.84591803851268	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	0.0
12	3.0
13	9.0
14	22.0
15	75.0
16	200.0
17	519.0
18	1142.0
19	2279.0
20	3864.0
21	6206.0
22	9314.0
23	13845.0
24	20233.0
25	28990.0
26	38003.0
27	45320.0
28	50544.0
29	56922.0
30	67151.0
31	81647.0
32	102248.0
33	132717.0
34	236052.0
35	415166.0
36	178635.0
37	211689.0
38	311146.0
39	576711.0
40	440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.55982509325208	24.950339528268938	28.656841991513243	13.832993386965741
2	29.11163041108103	27.078281575936042	29.67332344047594	14.13676457250699
3	28.56413987136712	25.5585765863467	31.647083568915846	14.23019997337033
4	25.565021737913895	28.85015794480712	30.70192331813384	14.882896999145148
5	21.946667335624515	33.55789733684021	30.20394852369365	14.291486803841618
6	21.100345606780145	40.805952695674996	27.19058930683746	10.903112390707403
7	86.60936785413116	4.891715664612837	6.769994924925562	1.7289215563304317
8	87.37495151663678	3.559537570023484	6.194987061454714	2.8705238518850136
9	82.22234229157944	5.2779616339810005	9.08025371512816	3.419442359311411
10	48.99492299587626	21.735675457673302	16.46601147391354	12.8033900725369
11	44.41346226209383	21.089577958353516	20.92640370190981	13.57055607764285
12	39.23993523973455	21.320792946611373	24.234503173368786	15.204768640285286
13	21.479528925029765	38.90420845241105	25.629203097532127	13.987059525027066
14	16.01265102205824	41.23206597982706	27.879757399863763	14.875525598250933
15	14.26775166483668	23.974034143865815	47.43214741258039	14.326066778717106
16	16.41919728917697	19.249931013721998	45.645914179140476	18.684957517960555
17	16.508194412014998	20.002238435873636	29.70720872835616	33.78235842375521
18	21.282623755593676	22.739343790945526	34.770705049409614	21.20732740405118
19	27.600068696825087	25.447233698494266	26.563286950111824	20.38941065456882
20	30.14601934703282	22.78747016222871	25.926953662447726	21.139556828290743
21	22.068739278181617	27.90260488326364	28.59771640947167	21.43093942908307
22	22.8454379326115	23.953116346563906	26.649273762637034	26.55217195818756
23	19.181195594912577	29.327176348223432	25.40474201061713	26.086886046246853
24	20.369766450091564	22.93335443123467	37.847126407947215	18.84975271072655
25	17.280030257478014	24.65675708532493	35.65589065626695	22.407322000930108
26	16.516106125016645	33.2574066176655	28.30691271450873	21.91957454280912
27	17.85168046713841	33.28473097281265	29.3846038064988	19.47898475355014
28	15.700582186295756	28.562055810381327	37.058772449485645	18.678589553837277
29	16.160079039942573	24.736105777673146	36.44277805329407	22.66103712909021
30	18.912969227295797	30.896590051696293	30.545387181867124	19.64505353914079
31	27.912793625860882	26.953508072841792	25.681574778230825	19.452123523066504
32	28.483401805028375	27.043238476397047	26.24299765157202	18.230362067002563
33	26.94683135894284	27.62341789861043	24.86022318749409	20.569527554952636
34	18.990504014711927	29.03753046491927	28.16751219079192	23.804453329576877
35	19.927289427828775	26.89909092487925	30.45866708862469	22.71495255866728
36	28.450172610421465	24.801097605452522	28.00584308950463	18.742886694621387
37	20.144263332683675	31.725197262161366	29.215949241536883	18.914590163618083
38	19.98201532556699	32.50289935336219	25.716965221267458	21.798120099803363
39	20.82312690194686	30.313593287779877	28.260523060713716	20.60275674955955
40	23.80638301567484	26.15855458792518	27.016415839635364	23.01864655676461
41	17.846740470727624	23.752621960985607	30.05104019729111	28.34959737099566
42	22.885189466229527	24.52773827281516	26.499684496322985	26.087387764632325
43	23.224544063417206	24.349821214583024	27.441255530962778	24.984379191036993
44	19.74914080726488	29.82947363952306	29.059374511548206	21.362011041663852
45	17.641537651070298	35.47955594063514	25.321495352351032	21.55741105594353
46	22.828804038447064	31.365040648837656	26.580885687325246	19.225269625390037
47	21.01640426151878	28.578033611272453	27.32613045835834	23.07943166885043
48	23.26383247237172	25.051725235855884	30.49340143838802	21.19104085338438
49	21.95164592575726	23.613105656102924	31.57981471154087	22.855433706598948
50	20.10852554614941	30.357898880589097	28.824647494592053	20.70892807866944
51	18.79514259415421	32.21375518844349	25.54070769307957	23.45039452432273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3106.0
1	3918.5
2	4731.0
3	12795.0
4	20859.0
5	14866.5
6	8874.0
7	9733.5
8	10593.0
9	11549.0
10	12505.0
11	12853.0
12	13201.0
13	12951.0
14	12701.0
15	12662.0
16	12623.0
17	12102.0
18	11581.0
19	11267.5
20	10954.0
21	11876.0
22	12798.0
23	11864.0
24	10930.0
25	13037.0
26	17163.5
27	19183.0
28	22312.5
29	25442.0
30	32721.0
31	40000.0
32	42943.0
33	45886.0
34	54887.0
35	63888.0
36	66431.0
37	68974.0
38	80792.5
39	92611.0
40	137363.5
41	182116.0
42	213575.5
43	245035.0
44	258893.0
45	272751.0
46	266277.5
47	259804.0
48	253611.0
49	247418.0
50	226405.5
51	205393.0
52	183269.0
53	161145.0
54	139882.0
55	118619.0
56	109130.0
57	99641.0
58	88013.5
59	76386.0
60	69240.5
61	62095.0
62	53984.5
63	45874.0
64	40061.5
65	34249.0
66	28479.5
67	22710.0
68	18956.0
69	15202.0
70	12604.0
71	10006.0
72	8162.0
73	6318.0
74	5181.0
75	3393.5
76	2743.0
77	2141.0
78	1539.0
79	1253.0
80	967.0
81	610.0
82	253.0
83	185.5
84	118.0
85	86.5
86	55.0
87	37.0
88	19.0
89	12.0
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2591095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.024469697881415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55463980804639	15.325093040519306
2	8.303370945348352	3.1593445788009737
3	2.710939430774647	1.5472255516069247
4	1.3017469838470415	0.9906018419402627
5	0.7804455865008265	0.7423781705615131
6	0.5323776803276006	0.6076921828332501
7	0.4015215964226365	0.5347114810931194
8	0.29014856311722637	0.44159380375260043
9	0.22855496771420283	0.39133233518212046
>10	2.53952636899919	11.258843499675868
>50	0.9774363270943177	13.527502272703554
>100	1.3675958735035325	48.9425976885584
>500	0.00923358024009244	1.0855252461556428
>1k	0.0020519067200205424	0.5639594084871465
>5k	0.0	0.0
>10k+	4.1038134400410854E-4	0.8815988981292806
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12288	0.4742396554352503	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10296	0.39736096129242654	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.057543239441240096	0.0
2	0.0	0.0	0.0	0.22824327166699793	0.0
3	0.0	0.0	0.0	0.33881428508024597	0.0
4	0.0	0.0	0.0	0.6120964302736874	0.0
5	0.0	0.0	0.0	1.0181023852849858	0.0
6	0.0	0.0	0.0	1.669101287293596	0.0
7	0.0	0.0	0.0	2.0031299508508953	0.0
8	0.0	0.0	0.0	2.969169405212854	0.0
9	0.0	0.0	0.0	3.3778769207612998	0.0
10	3.859372195924889E-5	0.0	0.0	4.024862075686148	0.0
11	3.859372195924889E-5	0.0	0.0	4.518051248603389	0.0
12	3.859372195924889E-5	0.0	0.0	4.957093429611805	0.0
13	3.859372195924889E-5	0.0	0.0	5.14693594792935	0.0
14	3.859372195924889E-5	0.0	0.0	5.222077924584008	0.0
15	3.859372195924889E-5	0.0	0.0	5.316246606164575	0.0
16	3.859372195924889E-5	0.0	0.0	5.528164733442811	0.0
17	3.859372195924889E-5	0.0	0.0	5.754516912733806	0.0
18	3.859372195924889E-5	0.0	0.0	6.107610874938974	0.0
19	3.859372195924889E-5	0.0	0.0	6.238134842605153	0.0
20	3.859372195924889E-5	0.0	0.0	6.385061142104014	0.0
21	3.859372195924889E-5	0.0	0.0	6.574942254143519	0.0
22	3.859372195924889E-5	0.0	0.0	6.763511179636408	0.0
23	3.859372195924889E-5	0.0	0.0	6.988666953546667	0.0
24	3.859372195924889E-5	0.0	0.0	7.158170580391688	0.0
25	3.859372195924889E-5	0.0	0.0	7.299925321148009	0.0
26	3.859372195924889E-5	0.0	0.0	7.44376412289013	0.0
27	3.859372195924889E-5	0.0	0.0	7.5782246501961525	0.0
28	3.859372195924889E-5	0.0	0.0	7.711565959565357	0.0
29	3.859372195924889E-5	0.0	0.0	7.859881633054751	0.0
30	3.859372195924889E-5	0.0	0.0	8.072726009660009	0.0
31	3.859372195924889E-5	0.0	0.0	8.254155096590438	0.0
32	3.859372195924889E-5	0.0	0.0	8.413431387116258	0.0
33	3.859372195924889E-5	0.0	0.0	8.571395491095464	0.0
34	3.859372195924889E-5	0.0	0.0	8.741053492828321	0.0
35	7.718744391849778E-5	0.0	0.0	8.95235412055521	0.0
36	7.718744391849778E-5	0.0	0.0	9.1265275877573	3.859372195924889E-5
37	7.718744391849778E-5	0.0	0.0	9.309037298902588	3.859372195924889E-5
38	7.718744391849778E-5	0.0	0.0	9.480817955343204	3.859372195924889E-5
39	1.1578116587774667E-4	0.0	0.0	9.665604696084088	3.859372195924889E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAC	30	2.16656E-6	45.000004	29
ACGTTAC	45	3.8562575E-10	45.0	39
CCTACGT	20	7.034927E-4	45.0	29
GCGATCG	35	1.2127202E-7	45.0	9
CGTAATC	20	7.034927E-4	45.0	35
ACGTATA	20	7.034927E-4	45.0	10
TACGTAA	25	3.892165E-5	45.0	10
TACGTCA	65	0.0	44.999996	38
GTTACAC	135	0.0	43.333332	33
GCGTAAG	270	0.0	43.333332	1
CGAATAT	210	0.0	42.857143	14
GTCAACG	145	0.0	41.89655	1
CGTTTTT	8220	0.0	41.687958	1
TAACGTT	55	6.184564E-11	40.909092	36
TCGATTG	110	0.0	40.909092	1
TATTACG	50	1.0822987E-9	40.5	1
CGTACCG	50	1.0822987E-9	40.5	34
CGACGGT	340	0.0	40.367645	28
ATCGTAC	45	1.9299478E-8	40.0	28
TACGCGC	45	1.9299478E-8	40.0	34
>>END_MODULE