FastQCFastQC Report
Thu 26 May 2016
SRR1546681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546681_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1407931
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140971.0012564536188209No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30730.2182635370625407No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC15220.10810188851584346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG302.1658507E-645.0000041
CCGGTCT302.1658507E-645.00000423
CACGCTA207.033829E-445.038
CCGTCGA207.033829E-445.017
AACGCAT207.033829E-445.044
CGCAAGT207.033829E-445.016
ACGGGTA850.042.3529435
CGGTCTA850.042.35294331
ATACCGG700.041.7857132
TCGATAG650.041.5384641
CGTTTTT68400.041.3486821
CGTTGAT1200.041.25000425
ACTACGG603.6379788E-1241.2500042
TCGTTGA1100.040.9090924
TATAGCG900.040.01
TACGTAG451.9288564E-840.01
CGTTCGG3100.039.91935345
AGGGCGA16050.039.5327076
CATACGA1150.039.13043218
ATACGAA1150.039.13043219
Produced by FastQC (version 0.11.5)