##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546681_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1407931
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.241469929989467	31.0	31.0	33.0	30.0	34.0
2	31.629000995077174	31.0	31.0	34.0	30.0	34.0
3	31.695146992288684	31.0	31.0	34.0	30.0	34.0
4	35.48815957600195	37.0	35.0	37.0	33.0	37.0
5	35.39535460189455	37.0	35.0	37.0	33.0	37.0
6	35.46248999418295	37.0	35.0	37.0	33.0	37.0
7	35.9145291921266	37.0	35.0	37.0	35.0	37.0
8	35.89957320351637	37.0	35.0	37.0	35.0	37.0
9	37.59189619377654	39.0	37.0	39.0	35.0	39.0
10	37.004480333198146	39.0	37.0	39.0	33.0	39.0
11	36.71306548403295	39.0	35.0	39.0	32.0	39.0
12	36.21695452404983	38.0	35.0	39.0	32.0	39.0
13	36.03842659903078	38.0	35.0	39.0	31.0	39.0
14	37.064430714289266	39.0	35.0	41.0	31.0	41.0
15	37.28164590452231	39.0	35.0	41.0	32.0	41.0
16	37.362238632432984	39.0	35.0	41.0	32.0	41.0
17	37.29062361720852	39.0	35.0	41.0	32.0	41.0
18	37.17123282319943	39.0	35.0	41.0	32.0	41.0
19	37.11107788662939	38.0	35.0	41.0	32.0	41.0
20	36.93348750755541	38.0	35.0	41.0	32.0	41.0
21	36.837256939438085	38.0	35.0	41.0	31.0	41.0
22	36.72792416673828	38.0	35.0	41.0	31.0	41.0
23	36.646877581358744	38.0	35.0	40.0	31.0	41.0
24	36.52429700035016	38.0	35.0	40.0	31.0	41.0
25	36.43642337586146	38.0	35.0	40.0	31.0	41.0
26	36.26527223280118	38.0	35.0	40.0	31.0	41.0
27	36.15667316082961	38.0	35.0	40.0	30.0	41.0
28	36.06886914202472	38.0	35.0	40.0	30.0	41.0
29	36.116595912725835	38.0	35.0	40.0	30.0	41.0
30	36.083461476450196	38.0	35.0	40.0	30.0	41.0
31	36.00937901076118	38.0	35.0	40.0	30.0	41.0
32	35.83983945235953	38.0	35.0	40.0	30.0	41.0
33	35.69923170950849	38.0	35.0	40.0	29.0	41.0
34	35.55061007961328	38.0	35.0	40.0	29.0	41.0
35	35.42970358632632	38.0	35.0	40.0	27.0	41.0
36	35.28242577228571	38.0	34.0	40.0	27.0	41.0
37	35.23453422078212	38.0	34.0	40.0	27.0	41.0
38	35.16722978611878	38.0	34.0	40.0	27.0	41.0
39	35.09951410971134	37.0	34.0	40.0	26.0	41.0
40	34.97909911778347	37.0	34.0	40.0	25.0	41.0
41	34.938266861089076	37.0	34.0	40.0	25.0	41.0
42	34.856716699895095	37.0	34.0	40.0	25.0	41.0
43	34.74462740006435	37.0	34.0	40.0	25.0	41.0
44	34.62075911390544	37.0	34.0	40.0	24.0	41.0
45	34.50258144752832	36.0	34.0	40.0	24.0	41.0
46	34.46955213004046	36.0	34.0	40.0	24.0	41.0
47	34.427287274731505	36.0	33.0	40.0	24.0	41.0
48	34.34810583757301	36.0	33.0	40.0	23.0	41.0
49	34.27602133911392	36.0	33.0	40.0	24.0	41.0
50	34.15590536752156	36.0	33.0	40.0	24.0	41.0
51	33.80146186141224	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	4.0
14	15.0
15	39.0
16	99.0
17	246.0
18	590.0
19	1231.0
20	2133.0
21	3510.0
22	5351.0
23	7965.0
24	11737.0
25	17262.0
26	23144.0
27	26044.0
28	27798.0
29	30744.0
30	36292.0
31	43763.0
32	53909.0
33	69711.0
34	111409.0
35	178118.0
36	112692.0
37	131302.0
38	182420.0
39	330183.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.5506718724142	24.043294735324388	28.311614702709154	15.094418689552258
2	31.904049275142032	25.898854418291805	28.97144817466197	13.225648131904192
3	27.21468594696757	25.985293313379703	32.836907490494916	13.963113249157807
4	24.203671912899143	28.27255028833089	32.639454632364796	14.884323166405173
5	22.415445075078253	32.05242302357147	30.85492115735785	14.677210743992426
6	20.224854769161276	41.05265101769902	28.01223923615575	10.710254976983958
7	85.39495188329542	3.7543743265827656	8.904129534757029	1.946544255364787
8	85.19742799895734	3.2490938831519443	8.281726874399386	3.271751243491336
9	78.82389122762407	5.834234774289365	11.429253280167849	3.912620717918705
10	42.37572721958676	29.13004969703771	16.27217526995286	12.222047813422675
11	34.12795087259248	23.335873704038054	27.318242158173945	15.217933265195525
12	30.944343153180093	22.07118104509383	29.406696777043763	17.577779024682314
13	21.051812908445086	33.302342231259914	30.81720624093084	14.82863861936416
14	16.560896805312193	36.62778928796937	30.382880979252537	16.428432927465906
15	15.086818885300488	25.568866656107435	44.1005986799069	15.243715778685177
16	17.175912740041948	22.723840870042636	41.899070337964005	18.20117605195141
17	17.05630460583651	23.949042957360835	30.820899603744785	28.17375283305787
18	20.46485232585972	25.09476671797126	34.49643483949142	19.943946116677594
19	24.809738545425876	28.223116047590402	27.928002153514626	19.0391432534691
20	26.846557111108428	25.990265147936935	27.139256114113547	20.023921626841087
21	21.33762236927804	30.605264036376784	28.510630137414406	19.546483456930773
22	21.296711273492804	26.05546720684465	27.790708493527028	24.857113026135515
23	18.22773985372863	31.23121800713245	26.847622504227836	23.693419634911088
24	19.27360076594663	27.04138199954401	35.48192347494302	18.20309375956634
25	18.536845910772616	28.349400645344126	32.174517075055526	20.939236368827736
26	17.70974571907288	33.37216099368506	29.029192481733833	19.888900805508225
27	18.7198094224788	31.515109760350473	29.790451378654208	19.974629438516516
28	17.150059200344337	29.40683882945968	35.42091196230497	18.022190007891012
29	17.11198915287752	26.251286462191686	34.58706428084899	22.049660104081806
30	19.246255675881844	29.8005371001846	31.545580003565515	19.40762722036804
31	24.254952835046602	27.972961743153608	27.543679342240495	20.228406079559296
32	24.282226898903424	27.778420959549866	28.328376887787826	19.610975253758884
33	22.536473733442904	28.74459046643621	28.96171758417138	19.757218215949504
34	18.18476899791254	28.74778664579443	29.990319127854985	23.077125228438042
35	18.09371339930721	27.284007525936993	31.979195003164218	22.643084071591577
36	22.986495787080475	28.565320317543968	29.161656359580125	19.286527535795432
37	18.639904938523266	30.842207466132926	30.91131596647847	19.606571628865336
38	18.74111728486694	32.39427216248524	26.92468593986495	21.93992461278287
39	18.47064948495345	31.042927529829235	28.95944474551665	21.526978239700668
40	21.425055631277385	27.606111379037753	28.428594867220053	22.54023812246481
41	17.29140135418568	26.62140403187372	28.9791190051217	27.1080756088189
42	20.961041414671598	27.815212535273393	26.865165977594074	24.358580072460935
43	20.99612836140407	26.89137464833149	29.71033381607479	22.402163174189642
44	20.002897869284787	30.664926051063585	28.8235005834803	20.508675496171332
45	17.813088851655372	33.39915095271004	26.585535796853684	22.202224398780906
46	21.33023564365015	30.950735511896532	26.88235431992051	20.836674524532807
47	20.427776645304352	28.44216087294051	28.415383992539407	22.714678489215736
48	21.260558933640926	26.6358223520897	30.047921382510935	22.055697331758445
49	20.492126389716542	25.88912382780122	30.793199382640204	22.825550399842037
50	18.571151569217527	30.186493514241818	29.684054119129417	21.55830079741124
51	18.457083479233003	31.066153099832306	27.404894131885726	23.071869289048966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1561.0
1	1737.0
2	1913.0
3	10747.0
4	19581.0
5	13196.0
6	6811.0
7	7178.0
8	7545.0
9	8777.5
10	10010.0
11	11194.5
12	12379.0
13	12757.0
14	13135.0
15	12801.5
16	12468.0
17	11702.0
18	10936.0
19	10147.5
20	9359.0
21	9188.5
22	9018.0
23	8877.0
24	8736.0
25	9624.0
26	11910.5
27	13309.0
28	15390.0
29	17471.0
30	19707.5
31	21944.0
32	25589.0
33	29234.0
34	32705.5
35	36177.0
36	41148.0
37	46119.0
38	55016.0
39	63913.0
40	76820.0
41	89727.0
42	105904.5
43	122082.0
44	136495.5
45	150909.0
46	148919.0
47	146929.0
48	134980.0
49	123031.0
50	110981.0
51	98931.0
52	88441.0
53	77951.0
54	67723.5
55	57496.0
56	51842.5
57	46189.0
58	40527.0
59	34865.0
60	31452.5
61	28040.0
62	24801.5
63	21563.0
64	19134.0
65	16705.0
66	14198.5
67	11692.0
68	9380.5
69	7069.0
70	6002.5
71	4936.0
72	4156.5
73	3377.0
74	2704.0
75	1603.0
76	1175.0
77	867.5
78	560.0
79	453.0
80	346.0
81	230.0
82	114.0
83	71.5
84	29.0
85	22.0
86	15.0
87	22.0
88	29.0
89	17.0
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1407931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.565920747760714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26204288105514	22.07061393750311
2	7.845674512674244	4.482378326835573
3	3.0915990950836085	2.649431242020213
4	1.8370189186976051	2.099045473746115
5	1.1686040619806706	1.6691125510025544
6	0.8688284961601582	1.489133157882432
7	0.706901359990137	1.4135301758173766
8	0.5362849472161392	1.2255578640314617
9	0.4648353673959385	1.1950605239209755
>10	4.997573519259447	31.934640881800586
>50	1.0136104910906907	20.018575486462822
>100	0.20301615378970087	7.650479431510153
>500	0.0022557350287034606	0.4279342599355703
>1k	0.0015038233524689735	0.6652061584509592
>5k	0.0	0.0
>10k+	2.5063722541149563E-4	1.0093005290800963
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14097	1.0012564536188209	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3073	0.2182635370625407	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	1522	0.10810188851584346	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.102620796047533E-5	0.0	0.0	0.09659564282624646	0.0
2	7.102620796047533E-5	0.0	0.0	0.34426402998442396	0.0
3	7.102620796047533E-5	0.0	0.0	0.5390889184200078	0.0
4	7.102620796047533E-5	0.0	0.0	0.8822165290770642	0.0
5	7.102620796047533E-5	0.0	0.0	1.5557580591662517	0.0
6	7.102620796047533E-5	0.0	0.0	2.671224655185517	0.0
7	7.102620796047533E-5	0.0	0.0	3.3080456357591386	0.0
8	7.102620796047533E-5	0.0	0.0	4.963382438485977	0.0
9	7.102620796047533E-5	0.0	0.0	5.733945768649174	0.0
10	7.102620796047533E-5	0.0	0.0	6.838829459682328	0.0
11	7.102620796047533E-5	0.0	0.0	7.520823108518813	0.0
12	7.102620796047533E-5	0.0	0.0	8.191949747537343	0.0
13	7.102620796047533E-5	0.0	0.0	8.520659037978424	0.0
14	7.102620796047533E-5	0.0	0.0	8.667044052584963	0.0
15	7.102620796047533E-5	0.0	0.0	8.816483194133804	0.0
16	7.102620796047533E-5	0.0	0.0	9.07587090560546	0.0
17	7.102620796047533E-5	0.0	0.0	9.362035497478214	0.0
18	7.102620796047533E-5	0.0	0.0	9.806020323439146	0.0
19	7.102620796047533E-5	0.0	0.0	10.0053908891842	0.0
20	7.102620796047533E-5	0.0	0.0	10.205116585969057	0.0
21	7.102620796047533E-5	0.0	0.0	10.43275558248238	0.0
22	7.102620796047533E-5	0.0	0.0	10.688805062179894	0.0
23	7.102620796047533E-5	0.0	0.0	10.982072274848697	0.0
24	7.102620796047533E-5	0.0	0.0	11.202892755397814	0.0
25	1.4205241592095066E-4	0.0	0.0	11.388697315422418	0.0
26	1.4205241592095066E-4	0.0	0.0	11.566760018779329	0.0
27	1.4205241592095066E-4	0.0	0.0	11.760093356847744	0.0
28	1.4205241592095066E-4	0.0	0.0	11.955983638402735	0.0
29	1.4205241592095066E-4	0.0	0.0	12.166505318797583	0.0
30	1.4205241592095066E-4	0.0	0.0	12.487827883610773	0.0
31	1.4205241592095066E-4	0.0	0.0	12.7158220111639	0.0
32	1.4205241592095066E-4	0.0	0.0	12.93571915100953	0.0
33	1.4205241592095066E-4	0.0	0.0	13.148229565227274	0.0
34	1.4205241592095066E-4	0.0	0.0	13.36294179189179	0.0
35	1.4205241592095066E-4	0.0	0.0	13.612101729417137	0.0
36	1.4205241592095066E-4	0.0	0.0	13.83526607482895	0.0
37	1.4205241592095066E-4	0.0	0.0	14.06517790999701	0.0
38	1.4205241592095066E-4	0.0	0.0	14.292816906510334	0.0
39	1.4205241592095066E-4	0.0	0.0	14.52976033626648	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTCG	30	2.1658507E-6	45.000004	1
CCGGTCT	30	2.1658507E-6	45.000004	23
CACGCTA	20	7.033829E-4	45.0	38
CCGTCGA	20	7.033829E-4	45.0	17
AACGCAT	20	7.033829E-4	45.0	44
CGCAAGT	20	7.033829E-4	45.0	16
ACGGGTA	85	0.0	42.352943	5
CGGTCTA	85	0.0	42.352943	31
ATACCGG	70	0.0	41.785713	2
TCGATAG	65	0.0	41.538464	1
CGTTTTT	6840	0.0	41.348682	1
CGTTGAT	120	0.0	41.250004	25
ACTACGG	60	3.6379788E-12	41.250004	2
TCGTTGA	110	0.0	40.90909	24
TATAGCG	90	0.0	40.0	1
TACGTAG	45	1.9288564E-8	40.0	1
CGTTCGG	310	0.0	39.919353	45
AGGGCGA	1605	0.0	39.532707	6
CATACGA	115	0.0	39.130432	18
ATACGAA	115	0.0	39.130432	19
>>END_MODULE