FastQCFastQC Report
Thu 26 May 2016
SRR1546680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546680_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2930281
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156870.53534114987607No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC84800.28939204124109597No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29480.10060468603523007No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA207.0350774E-445.00000442
TGTCGAT207.0350774E-445.00000412
TACGTAG900.045.01
GCGATCG700.041.7857139
TGATTCG4050.041.1111115
CGGTCTA2150.040.81395331
TACGAAT3500.040.512
CGTTTTT97250.039.655531
GCTACGA3600.039.37510
CGGTAGT3100.039.1935512
ACGTAAG1050.038.571431
CGCATCG700.038.57142621
CGAGACA6850.038.4306622
GCGGGAT13550.038.3579335
TATACGG2000.038.252
ATTACCG659.094947E-1238.076921
CGAGGGA14300.037.9195824
GCGAGAC6950.037.87769721
GGGCGAT63150.037.767227
TACGGGA7150.037.7622384
Produced by FastQC (version 0.11.5)