##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546680_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2930281
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.344198730428925	31.0	31.0	33.0	30.0	34.0
2	31.756779981169043	31.0	31.0	34.0	30.0	34.0
3	31.868677099568266	31.0	31.0	34.0	30.0	34.0
4	35.618551258394675	37.0	35.0	37.0	33.0	37.0
5	35.53074124972998	37.0	35.0	37.0	33.0	37.0
6	35.58519302415024	37.0	35.0	37.0	33.0	37.0
7	35.96245854919716	37.0	35.0	37.0	35.0	37.0
8	35.938931795278336	37.0	35.0	37.0	35.0	37.0
9	37.6493353367817	39.0	37.0	39.0	35.0	39.0
10	37.095254004650066	39.0	37.0	39.0	33.0	39.0
11	36.82433493579626	39.0	37.0	39.0	32.0	39.0
12	36.1981151295729	38.0	35.0	39.0	32.0	39.0
13	35.958801562034495	38.0	35.0	39.0	31.0	39.0
14	36.97349196203368	39.0	35.0	41.0	31.0	41.0
15	37.20772035173419	39.0	35.0	41.0	32.0	41.0
16	37.298220546084146	39.0	35.0	41.0	32.0	41.0
17	37.21300448660043	39.0	35.0	41.0	32.0	41.0
18	37.13593747493841	39.0	35.0	41.0	32.0	41.0
19	37.08360153855552	38.0	35.0	41.0	32.0	41.0
20	36.94046202394924	38.0	35.0	41.0	32.0	41.0
21	36.821166297703186	38.0	35.0	41.0	32.0	41.0
22	36.71289204004667	38.0	35.0	41.0	31.0	41.0
23	36.65509860658415	38.0	35.0	41.0	31.0	41.0
24	36.528941422341404	38.0	35.0	41.0	31.0	41.0
25	36.43577015310136	38.0	35.0	40.0	31.0	41.0
26	36.28944596098463	38.0	35.0	40.0	31.0	41.0
27	36.18980159240701	38.0	35.0	40.0	30.0	41.0
28	36.146852127833476	38.0	35.0	40.0	30.0	41.0
29	36.186805292734725	38.0	35.0	40.0	30.0	41.0
30	36.16625231505101	38.0	35.0	40.0	30.0	41.0
31	36.08609140215563	38.0	35.0	40.0	30.0	41.0
32	35.95810094663276	38.0	35.0	40.0	30.0	41.0
33	35.8692145906826	38.0	35.0	40.0	30.0	41.0
34	35.75221079480091	38.0	35.0	40.0	29.0	41.0
35	35.66366092535153	38.0	35.0	40.0	29.0	41.0
36	35.52888784386207	38.0	35.0	40.0	29.0	41.0
37	35.47247653040783	38.0	34.0	40.0	28.0	41.0
38	35.42434394517113	38.0	34.0	40.0	28.0	41.0
39	35.36176257498854	37.0	34.0	40.0	28.0	41.0
40	35.206768907145765	37.0	34.0	40.0	27.0	41.0
41	35.18267633718404	37.0	34.0	40.0	27.0	41.0
42	35.114555907778126	37.0	34.0	40.0	27.0	41.0
43	34.99372346884138	37.0	34.0	40.0	27.0	41.0
44	34.85412866547611	37.0	34.0	40.0	26.0	41.0
45	34.75116140738721	36.0	34.0	40.0	26.0	41.0
46	34.71189213594191	36.0	34.0	40.0	26.0	41.0
47	34.68243045632825	36.0	34.0	40.0	26.0	41.0
48	34.610888170793174	36.0	34.0	40.0	25.0	41.0
49	34.55655549757856	36.0	34.0	40.0	25.0	41.0
50	34.43602473619424	36.0	34.0	40.0	24.0	41.0
51	34.061972213586344	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	6.0
13	14.0
14	33.0
15	88.0
16	240.0
17	602.0
18	1411.0
19	2585.0
20	4359.0
21	6960.0
22	10612.0
23	15651.0
24	22359.0
25	31437.0
26	41214.0
27	49299.0
28	54531.0
29	62239.0
30	73225.0
31	88190.0
32	109775.0
33	141000.0
34	243205.0
35	401138.0
36	210267.0
37	255871.0
38	379724.0
39	723663.0
40	575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.36266419500382	24.81570880062356	28.912210125923078	13.909416878449541
2	31.15571510036068	26.525032923463655	28.578010095277552	13.74124188089811
3	28.974251957406132	26.086167162807932	30.607269405220865	14.332311474565069
4	25.07356802982376	29.10437599670475	30.565225655833007	15.256830317638478
5	23.79358839647119	32.15067087422674	28.889345424551433	15.166395304750639
6	21.440230476189825	40.614364287930066	26.814834481744242	11.130570754135865
7	86.52927825010639	4.774968680478084	6.907801674992944	1.7879513944225827
8	86.83058041191272	3.670876615587379	6.716250079770507	2.7822928927294
9	80.97336057531683	6.16456237473471	9.427150501948448	3.4349265480000044
10	45.21945164985884	27.814704460084204	14.791755466455264	12.174088423601695
11	38.40519049196988	23.04867690163503	24.006810268366756	14.539322338028333
12	35.56324461715447	22.138013385064436	26.0062089608471	16.292533036934
13	22.204867041761524	35.436942736891105	26.90567901167158	15.452511209675796
14	17.27363348429724	37.559913196038195	28.363184281643978	16.803269038020584
15	16.016962195775765	24.212626707131502	43.57223761134171	16.198173485751024
16	18.58060711583633	21.38129414892292	41.13810245502052	18.899996280220225
17	18.470276400113164	22.280320556287947	29.07021545032712	30.179187593271774
18	21.510735659822387	24.238665165559205	34.085434127307245	20.165165047311163
19	27.104533660764957	25.692826046375757	26.936938812352807	20.265701480506475
20	28.518493618871364	24.006332498487346	26.70191015810429	20.773263724536996
21	23.03946276824646	28.091742737300617	28.451810594274065	20.416983900178856
22	22.924763870768707	24.93979246358967	26.642188923178356	25.493254742463268
23	20.483769304036027	29.697561428409085	25.983173627375667	23.835495640179218
24	20.49066284086748	24.694491756933893	36.585228515627	18.229616886571627
25	18.946578843462454	26.485992299032073	32.87677871166622	21.690650145839253
26	18.021513977669716	32.191247187556414	28.310220077869662	21.4770187569042
27	18.617020005931174	32.38150197882046	28.51453495415627	20.486943061092095
28	17.026285192444	29.418339060315375	34.31408114102368	19.241294606216947
29	17.334992787381143	25.50345854203061	33.737003379539374	23.42454529104888
30	19.634055573509844	29.51597474781429	30.97805295806102	19.87191672061485
31	26.60662919358246	26.95335362035245	26.51571641081521	19.924300775249883
32	26.1371520342247	27.580801977694293	26.71774481696465	19.564301171116355
33	25.069131595229262	28.091401473101048	26.385524118676674	20.45394281299302
34	19.160210232397507	29.018889314710773	28.76669507122355	23.054205381668176
35	19.580988990475657	27.31823330253993	30.341424593750567	22.75935311323385
36	25.67688900825552	28.116655023869725	26.723921698976994	19.482534268897762
37	19.67159463546329	31.919737390373143	29.333705538820336	19.07496243534323
38	19.45803149936815	32.44381682166318	25.86301450270469	22.235137176263983
39	20.78029376704828	31.165577635728454	27.160978759374956	20.893149837848316
40	22.911249808465467	27.266600029143962	27.105489200523774	22.716660961866797
41	18.016838658135516	25.93198399743915	28.55715885268341	27.49401849174192
42	21.49374070268346	26.73023508666916	26.203937438081876	25.5720867725655
43	21.34672408550579	26.47988366985965	27.77508368651334	24.39830855812122
44	19.434313637497564	30.21266561125025	28.4968574686182	21.856163282633986
45	18.46679550527748	33.39597806490231	25.835849872418382	22.301376557401834
46	21.997037144219274	31.006446139465805	26.570421062007366	20.426095654307556
47	21.426409276106966	28.60316126678636	27.398464515860425	22.57196494124625
48	22.168385898826767	25.752513154881733	30.539835599384496	21.539265346907
49	21.35382238085699	25.295901655848024	30.521680343967013	22.828595619327977
50	19.73930145265932	30.766264395803677	28.584596494329382	20.909837657207618
51	18.960263537865483	31.09991840372988	26.63447635226792	23.305341706136716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3236.0
1	4155.0
2	5074.0
3	16030.0
4	26986.0
5	19245.5
6	11505.0
7	12016.0
8	12527.0
9	13431.5
10	14336.0
11	14790.5
12	15245.0
13	15358.0
14	15471.0
15	15096.5
16	14722.0
17	14153.0
18	13584.0
19	13154.5
20	12725.0
21	13641.5
22	14558.0
23	15268.0
24	15978.0
25	19467.5
26	23785.0
27	24613.0
28	29083.5
29	33554.0
30	42018.0
31	50482.0
32	58121.5
33	65761.0
34	73542.5
35	81324.0
36	85996.0
37	90668.0
38	106386.0
39	122104.0
40	157527.5
41	192951.0
42	228516.0
43	264081.0
44	281308.0
45	298535.0
46	291261.0
47	283987.0
48	260087.5
49	236188.0
50	218430.0
51	200672.0
52	186526.0
53	172380.0
54	156691.5
55	141003.0
56	126279.5
57	111556.0
58	100116.0
59	88676.0
60	80200.5
61	71725.0
62	65493.0
63	59261.0
64	52448.0
65	45635.0
66	38491.0
67	31347.0
68	26806.0
69	22265.0
70	18696.0
71	15127.0
72	13380.0
73	11633.0
74	8995.0
75	5791.5
76	5226.0
77	3668.0
78	2110.0
79	1646.5
80	1183.0
81	857.0
82	531.0
83	390.0
84	249.0
85	178.5
86	108.0
87	81.5
88	55.0
89	35.5
90	16.0
91	12.5
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2930281.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.769363383781418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.20529798613752	15.856076811924247
2	7.760366276176331	3.0683500180994505
3	2.5958921022151	1.539574028213357
4	1.323667056573251	1.046722201621478
5	0.8035281685636111	0.7942620176719197
6	0.5819256805514108	0.6902580144705086
7	0.40764223778220504	0.5641179270505969
8	0.3652578985365609	0.5776732899972508
9	0.2827873813180778	0.5031473851462536
>10	3.2306467233944742	15.103315181381666
>50	1.2145167457821324	17.397527732169188
>100	1.220595816557186	40.65720998608713
>500	0.005425638195066638	0.695053956675432
>1k	0.002100247043251602	0.6705222334932894
>5k	1.750205869376335E-4	0.29341186542252407
>10k+	1.750205869376335E-4	0.5427773505758414
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15687	0.53534114987607	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8480	0.28939204124109597	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2948	0.10060468603523007	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.825283991535283E-5	0.0	0.0	0.041258841728830785	0.0
2	6.825283991535283E-5	0.0	0.0	0.16878927311066755	0.0
3	6.825283991535283E-5	0.0	0.0	0.26727812110852167	0.0
4	6.825283991535283E-5	0.0	0.0	0.49012364343214865	0.0
5	6.825283991535283E-5	0.0	0.0	0.8287942350921293	0.0
6	6.825283991535283E-5	0.0	0.0	1.4614639346874925	0.0
7	6.825283991535283E-5	0.0	0.0	1.7720484827223055	0.0
8	6.825283991535283E-5	0.0	0.0	2.670119350328518	0.0
9	6.825283991535283E-5	0.0	0.0	3.075711851525502	0.0
10	6.825283991535283E-5	0.0	0.0	3.690772318422704	0.0
11	6.825283991535283E-5	0.0	0.0	4.145302105838996	0.0
12	6.825283991535283E-5	0.0	0.0	4.55751513250777	0.0
13	6.825283991535283E-5	0.0	0.0	4.749476244769699	0.0
14	6.825283991535283E-5	0.0	0.0	4.83465578898406	0.0
15	6.825283991535283E-5	0.0	0.0	4.924203514953003	0.0
16	6.825283991535283E-5	0.0	0.0	5.096644314999142	0.0
17	6.825283991535283E-5	0.0	0.0	5.279254788192668	0.0
18	6.825283991535283E-5	0.0	0.0	5.56298184372079	0.0
19	6.825283991535283E-5	0.0	0.0	5.687167885946774	0.0
20	6.825283991535283E-5	0.0	0.0	5.8235370600976495	0.0
21	6.825283991535283E-5	0.0	0.0	5.991029529249925	0.0
22	6.825283991535283E-5	0.0	0.0	6.167292488331324	0.0
23	6.825283991535283E-5	0.0	0.0	6.373689076235351	0.0
24	6.825283991535283E-5	0.0	0.0	6.536949869312875	0.0
25	6.825283991535283E-5	0.0	0.0	6.67140796394612	0.0
26	6.825283991535283E-5	0.0	0.0	6.80491051882055	0.0
27	6.825283991535283E-5	0.0	0.0	6.9427471290296054	0.0
28	6.825283991535283E-5	0.0	0.0	7.085668575812353	0.0
29	6.825283991535283E-5	0.0	0.0	7.23759939746393	0.0
30	6.825283991535283E-5	0.0	0.0	7.462799642764636	0.0
31	6.825283991535283E-5	0.0	0.0	7.638038809247305	0.0
32	6.825283991535283E-5	0.0	0.0	7.7985353623082565	0.0
33	1.3650567983070565E-4	0.0	0.0	7.9561311696728065	0.0
34	1.3650567983070565E-4	0.0	0.0	8.118129285211896	0.0
35	1.3650567983070565E-4	0.0	0.0	8.312991143170228	0.0
36	1.3650567983070565E-4	0.0	0.0	8.485466069636326	0.0
37	1.3650567983070565E-4	0.0	0.0	8.665687693432814	0.0
38	1.3650567983070565E-4	0.0	0.0	8.845533926609768	0.0
39	1.3650567983070565E-4	0.0	0.0	9.03421890255576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTA	20	7.0350774E-4	45.000004	42
TGTCGAT	20	7.0350774E-4	45.000004	12
TACGTAG	90	0.0	45.0	1
GCGATCG	70	0.0	41.785713	9
TGATTCG	405	0.0	41.11111	15
CGGTCTA	215	0.0	40.813953	31
TACGAAT	350	0.0	40.5	12
CGTTTTT	9725	0.0	39.65553	1
GCTACGA	360	0.0	39.375	10
CGGTAGT	310	0.0	39.19355	12
ACGTAAG	105	0.0	38.57143	1
CGCATCG	70	0.0	38.571426	21
CGAGACA	685	0.0	38.43066	22
GCGGGAT	1355	0.0	38.357933	5
TATACGG	200	0.0	38.25	2
ATTACCG	65	9.094947E-12	38.07692	1
CGAGGGA	1430	0.0	37.919582	4
GCGAGAC	695	0.0	37.877697	21
GGGCGAT	6315	0.0	37.76722	7
TACGGGA	715	0.0	37.762238	4
>>END_MODULE