FastQCFastQC Report
Thu 26 May 2016
SRR1546679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546679_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2213720
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442421.998536400267423No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30680.13859024628227598No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT22330.10087093218654572No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGG207.0347043E-445.038
CGTTTTT268550.043.810281
AGGGCGA22200.039.121626
ACGTCAT750.039.00000435
GCGCGAC2850.038.684219
TGATTCG2100.038.5714315
GTTTTTT312600.038.2629552
CTACGAA1500.037.50000411
CGGTCTA900.037.50000431
ACGGGTA2400.037.4999965
AGGGTAC9200.036.9293446
TACGGGA6350.036.8503954
AATACGG1650.036.818182
TAGGGCG8400.036.696435
GGGCGAT44950.036.690777
GACCGAT13950.036.6129049
GGACCGA14750.036.457638
TAGGGTA11100.036.2837835
TAGTACG751.8189894E-1236.0000041
CCAATCG250.002107529436.024
Produced by FastQC (version 0.11.5)