##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546679_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2213720 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30821422763493 31.0 31.0 33.0 30.0 34.0 2 31.69500388486349 31.0 31.0 34.0 30.0 34.0 3 31.597532659956997 31.0 31.0 34.0 30.0 34.0 4 35.50330077877961 37.0 35.0 37.0 33.0 37.0 5 35.46566187232351 37.0 35.0 37.0 33.0 37.0 6 35.54925103445784 37.0 35.0 37.0 33.0 37.0 7 35.96928879894476 37.0 35.0 37.0 35.0 37.0 8 35.95790976275229 37.0 35.0 37.0 35.0 37.0 9 37.67243056935836 39.0 37.0 39.0 35.0 39.0 10 37.08465027194045 39.0 37.0 39.0 33.0 39.0 11 36.85837052563107 39.0 37.0 39.0 32.0 39.0 12 36.53427849953924 39.0 35.0 39.0 32.0 39.0 13 36.456773214317984 39.0 35.0 39.0 32.0 39.0 14 37.57656207650471 40.0 36.0 41.0 32.0 41.0 15 37.70764821205934 40.0 36.0 41.0 33.0 41.0 16 37.72565590950978 40.0 36.0 41.0 33.0 41.0 17 37.64685732612977 40.0 36.0 41.0 32.0 41.0 18 37.41458359684152 39.0 36.0 41.0 32.0 41.0 19 37.22619391793 38.0 36.0 41.0 32.0 41.0 20 36.94263773196249 38.0 35.0 41.0 32.0 41.0 21 36.85756780441971 38.0 35.0 41.0 32.0 41.0 22 36.76059302892868 38.0 35.0 41.0 31.0 41.0 23 36.682760692409154 38.0 35.0 40.0 31.0 41.0 24 36.52666010154852 38.0 35.0 40.0 31.0 41.0 25 36.425255226496574 38.0 35.0 40.0 31.0 41.0 26 36.287091863469634 38.0 35.0 40.0 31.0 41.0 27 36.167718139602115 38.0 35.0 40.0 31.0 41.0 28 36.059778562781204 38.0 35.0 40.0 30.0 41.0 29 36.017899734383754 38.0 35.0 40.0 30.0 41.0 30 35.92951818658186 38.0 35.0 40.0 30.0 41.0 31 35.74033662793849 38.0 35.0 40.0 30.0 41.0 32 35.3723488065338 38.0 34.0 40.0 27.0 41.0 33 34.94986222286468 38.0 34.0 40.0 24.0 41.0 34 34.508850712827275 38.0 34.0 40.0 22.0 41.0 35 34.1849335055924 37.0 33.0 40.0 20.0 41.0 36 33.968862819146054 37.0 33.0 40.0 18.0 41.0 37 33.87946849646748 37.0 33.0 40.0 18.0 41.0 38 33.786454023092354 37.0 33.0 40.0 18.0 41.0 39 33.65845951610863 37.0 33.0 40.0 17.0 41.0 40 33.55035460672533 37.0 33.0 40.0 16.0 41.0 41 33.47537945178252 37.0 33.0 40.0 15.0 41.0 42 33.39289386191569 37.0 33.0 40.0 15.0 41.0 43 33.27289268742208 36.0 33.0 40.0 15.0 41.0 44 33.14018755759536 36.0 33.0 40.0 15.0 41.0 45 33.04387095025568 36.0 32.0 40.0 15.0 41.0 46 33.01582584970095 36.0 32.0 40.0 15.0 41.0 47 32.968025314854636 36.0 32.0 40.0 15.0 41.0 48 32.844129790578755 35.0 32.0 40.0 13.0 41.0 49 32.76021628751604 35.0 32.0 40.0 13.0 41.0 50 32.668609399562726 35.0 32.0 40.0 13.0 41.0 51 32.32227336790561 35.0 31.0 39.0 12.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 2.0 11 4.0 12 2.0 13 10.0 14 16.0 15 64.0 16 188.0 17 521.0 18 1266.0 19 2523.0 20 4636.0 21 7501.0 22 11318.0 23 17401.0 24 26871.0 25 44269.0 26 61915.0 27 65425.0 28 59317.0 29 55924.0 30 58834.0 31 67006.0 32 79144.0 33 99049.0 34 151170.0 35 228441.0 36 179060.0 37 207228.0 38 287526.0 39 496757.0 40 330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.623701281101496 22.57006306127243 25.912536364129156 20.89369929349692 2 38.58112137036301 23.652765480729272 25.8361491064814 11.929964042426324 3 25.83357425510001 23.528133639303977 37.78151708436478 12.85677502123123 4 22.47045696836095 25.884709900077695 37.98068409735649 13.664149034204867 5 20.494055255407186 29.889055526444174 36.31864011708798 13.298249101060659 6 18.73891006992754 37.941428906998176 33.61143234013335 9.708228682940932 7 79.41668323003812 3.5093869143342427 15.254052002963336 1.8198778526642936 8 79.64927813815659 3.0512440597726904 14.994714778743473 2.3047630233272502 9 74.12744159152919 5.405471333321287 17.262571598937534 3.2045154762119874 10 36.50624288527908 26.885288112317728 24.217064488733897 12.391404513669299 11 28.99756970167862 25.045534213902393 30.964259255913124 14.992636828505864 12 25.779863758740944 22.318314872702963 35.39684332255209 16.504978046004013 13 20.61200151780713 28.015964078564586 36.28932294960519 15.082711454023093 14 17.87714796812605 30.46898433406212 36.01449144426576 15.639376253546066 15 16.85822958639756 24.589198272590934 43.16891928518512 15.38365285582639 16 18.851977666552227 23.110149431725784 41.33580579296388 16.702067108758108 17 18.754946425022133 23.71980196230779 35.21484198543628 22.310409627233796 18 20.221030663317855 24.48986321666697 37.76909455577038 17.5200115642448 19 23.354353757476105 26.44322678568202 32.861021267368955 17.341398189472923 20 24.450924236127424 25.21737166398641 32.67368953616537 17.658014563720794 21 21.500054207397504 26.929196104295034 34.21873588349023 17.352013804817233 22 20.959651627125382 24.894024537881936 33.07450806786766 21.07181576712502 23 19.039444916249572 27.993016280288387 32.88812496612037 20.07941383734167 24 18.717046419601395 26.337657879045228 37.903709592902445 17.041586108450936 25 18.745821513109156 26.95964259255913 35.7913828307103 18.503153063621415 26 18.238801655132537 29.33943768859657 33.90704334784887 18.514717308422025 27 18.65122960446669 28.64910648139783 34.05828198688181 18.641381927253672 28 17.897656433514626 27.461422402110475 37.127730697649206 17.513190466725693 29 18.425862349348606 26.060883941961947 36.120060350902556 19.393193357786892 30 20.030446488264097 27.84186798691795 34.799929530383245 17.327755994434707 31 23.198417143992916 27.006170608749073 31.744258533147825 18.051153714110185 32 23.43254792837396 27.393392118244403 31.97500135518494 17.1990585981967 33 22.641797517301192 28.050205084653886 31.047512783911245 18.260484614133677 34 19.804988887483514 28.932294960518945 31.886146396111524 19.37656975588602 35 20.04210107872721 28.838561335670278 31.58687638906456 19.53246119653795 36 23.18418770214842 29.499891585204995 29.27854471206837 18.037376000578213 37 20.22721934119943 30.706457907955837 30.607032506369368 18.459290244475362 38 20.681838714923295 31.49693728204109 28.868917478271864 18.952306524763745 39 20.039752091502088 30.648501165459045 29.308855681838715 20.002891061200152 40 21.902860343674902 28.365375928301685 29.232694288347215 20.4990694396762 41 18.788464665811393 28.303398803823427 29.684874329183458 23.223262201181722 42 21.15195236976673 29.356738882966226 28.089640966337203 21.401667780929838 43 20.906347686247585 28.49249227544586 29.784435249263687 20.81672478904288 44 20.45601973149269 30.806877111829863 28.978416421227614 19.75868673544983 45 19.093065066946135 31.858319932060063 28.27046780984045 20.778147191153355 46 21.324467412319535 30.843692969300545 27.968758469905858 19.863081148474063 47 20.717209041793904 29.392922320799382 29.311159496232587 20.57870914117413 48 21.223506134470487 28.34378331496305 29.610068120629528 20.822642429936938 49 20.416132121496847 27.47529046130495 30.657084003396996 21.451493413801202 50 19.934634913177817 29.953336465316298 29.750781489980664 20.361247131525214 51 19.34612326762192 30.6589812623096 28.776855248179533 21.21804022188895 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2086.0 1 2592.0 2 3098.0 3 33137.5 4 63177.0 5 43148.5 6 23120.0 7 23872.0 8 24624.0 9 27305.5 10 29987.0 11 31778.5 12 33570.0 13 34341.5 14 35113.0 15 33649.5 16 32186.0 17 30863.0 18 29540.0 19 27322.0 20 25104.0 21 23733.5 22 22363.0 23 22373.5 24 22384.0 25 22759.5 26 23299.5 27 23464.0 28 26688.5 29 29913.0 30 33923.5 31 37934.0 32 43066.5 33 48199.0 34 54324.0 35 60449.0 36 65487.0 37 70525.0 38 77578.0 39 84631.0 40 99684.0 41 114737.0 42 127866.0 43 140995.0 44 150476.0 45 159957.0 46 169041.0 47 178125.0 48 176835.5 49 175546.0 50 164781.0 51 154016.0 52 140777.5 53 127539.0 54 114527.5 55 101516.0 56 91436.0 57 81356.0 58 73785.5 59 66215.0 60 59798.5 61 53382.0 62 47381.5 63 41381.0 64 36886.0 65 32391.0 66 27012.5 67 21634.0 68 17928.5 69 14223.0 70 11841.0 71 9459.0 72 8292.5 73 7126.0 74 5735.0 75 3537.0 76 2730.0 77 2112.0 78 1494.0 79 1023.0 80 552.0 81 351.5 82 151.0 83 124.0 84 97.0 85 100.0 86 103.0 87 66.0 88 29.0 89 18.0 90 7.0 91 6.5 92 6.0 93 5.5 94 5.0 95 3.0 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2213720.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.56713927864822 #Duplication Level Percentage of deduplicated Percentage of total 1 77.41038504918352 23.662140214121806 2 7.332444947837829 4.482637319471588 3 3.2682454750024146 2.9970274389363185 4 1.8959894420100722 2.318198933790736 5 1.3005272088885134 1.987669816488341 6 0.9840151401309017 1.804711670440789 7 0.7443300507401335 1.5926428231179872 8 0.5746061391677928 1.4051252709046598 9 0.5205216294827515 1.4319771431353374 >10 5.047080135130155 33.953133848246985 >50 0.8083455885807839 16.761956062338925 >100 0.11050256721992975 4.606801784241777 >500 0.0018039759750528635 0.3982658748302954 >1k 0.0010523193187808371 0.5647039732075113 >5k 0.0 0.0 >10k+ 1.503313312544053E-4 2.033007826726887 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44242 1.998536400267423 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 3068 0.13859024628227598 No Hit CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT 2233 0.10087093218654572 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.5172831252371576E-5 0.0 0.0 0.05330394087779846 0.0 2 4.5172831252371576E-5 0.0 0.0 0.16569394503369894 0.0 3 4.5172831252371576E-5 0.0 0.0 0.29805034060314767 0.0 4 4.5172831252371576E-5 0.0 0.0 0.46460256943064165 0.0 5 4.5172831252371576E-5 0.0 0.0 0.8258497009558571 0.0 6 4.5172831252371576E-5 0.0 0.0 1.5357407440868764 0.0 7 4.5172831252371576E-5 0.0 0.0 1.9749561823536852 0.0 8 4.5172831252371576E-5 0.0 0.0 2.865448204831686 0.0 9 4.5172831252371576E-5 0.0 0.0 3.36343349655783 0.0 10 4.5172831252371576E-5 0.0 0.0 4.0271579061489255 0.0 11 4.5172831252371576E-5 0.0 0.0 4.401279294581067 0.0 12 4.5172831252371576E-5 0.0 0.0 4.771425473863 0.0 13 4.5172831252371576E-5 0.0 0.0 4.981885694667799 0.0 14 4.5172831252371576E-5 0.0 0.0 5.093869143342428 0.0 15 4.5172831252371576E-5 0.0 0.0 5.187918978009866 0.0 16 4.5172831252371576E-5 0.0 0.0 5.345210776430624 0.0 17 4.5172831252371576E-5 0.0 0.0 5.506568129664095 0.0 18 4.5172831252371576E-5 0.0 0.0 5.763285329671323 0.0 19 4.5172831252371576E-5 0.0 0.0 5.885297146883978 0.0 20 4.5172831252371576E-5 0.0 0.0 6.0210415047973544 0.0 21 4.5172831252371576E-5 0.0 0.0 6.16419420703612 0.0 22 4.5172831252371576E-5 0.0 0.0 6.322073252263158 0.0 23 4.5172831252371576E-5 0.0 0.0 6.506965650579116 0.0 24 4.5172831252371576E-5 0.0 0.0 6.656442549193213 0.0 25 4.5172831252371576E-5 0.0 0.0 6.788482734943895 0.0 26 4.5172831252371576E-5 0.0 0.0 6.923504327557234 0.0 27 4.5172831252371576E-5 0.0 0.0 7.072213288040041 0.0 28 4.5172831252371576E-5 0.0 0.0 7.214236669497497 0.0 29 4.5172831252371576E-5 0.0 0.0 7.376723343512278 0.0 30 4.5172831252371576E-5 0.0 0.0 7.603490956399184 0.0 31 4.5172831252371576E-5 0.0 0.0 7.783956417252408 0.0 32 4.5172831252371576E-5 0.0 0.0 7.9575104349240195 0.0 33 9.034566250474315E-5 0.0 0.0 8.133458612652007 0.0 34 9.034566250474315E-5 0.0 0.0 8.313427172361456 0.0 35 9.034566250474315E-5 0.0 0.0 8.506315161809082 0.0 36 9.034566250474315E-5 0.0 0.0 8.702455595106878 0.0 37 9.034566250474315E-5 0.0 0.0 8.892317004860598 0.0 38 9.034566250474315E-5 0.0 0.0 9.08931572195219 0.0 39 9.034566250474315E-5 0.0 0.0 9.317212655620404 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTCGG 20 7.0347043E-4 45.0 38 CGTTTTT 26855 0.0 43.81028 1 AGGGCGA 2220 0.0 39.12162 6 ACGTCAT 75 0.0 39.000004 35 GCGCGAC 285 0.0 38.68421 9 TGATTCG 210 0.0 38.57143 15 GTTTTTT 31260 0.0 38.262955 2 CTACGAA 150 0.0 37.500004 11 CGGTCTA 90 0.0 37.500004 31 ACGGGTA 240 0.0 37.499996 5 AGGGTAC 920 0.0 36.929344 6 TACGGGA 635 0.0 36.850395 4 AATACGG 165 0.0 36.81818 2 TAGGGCG 840 0.0 36.69643 5 GGGCGAT 4495 0.0 36.69077 7 GACCGAT 1395 0.0 36.612904 9 GGACCGA 1475 0.0 36.45763 8 TAGGGTA 1110 0.0 36.283783 5 TAGTACG 75 1.8189894E-12 36.000004 1 CCAATCG 25 0.0021075294 36.0 24 >>END_MODULE