FastQCFastQC Report
Thu 26 May 2016
SRR1546678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546678_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1487414
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63940.4298735926917456No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55130.3706432775273058No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15160.10192185901168066No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC207.0339575E-445.044
CAACGAA351.2122473E-745.017
CTATCCG406.8175723E-945.029
CCGTGTA207.0339575E-445.034
ATCCGTA406.8175723E-945.035
CTAACGG351.2122473E-745.02
CTACGAT207.0339575E-445.021
ACGGATA207.0339575E-445.09
GTACTAA207.0339575E-445.023
CGTACAA207.0339575E-445.031
CGTCGAA253.8913604E-545.029
CCAACGC406.8175723E-945.020
CCGTTGA207.0339575E-445.032
TAATCCG253.8913604E-545.01
ACGTTAG302.1659343E-644.9999961
TGTAGCG1050.042.8571471
TGATTCG1000.042.7515
TCGTTAG950.042.631581
CGTTAGG2850.041.052632
TCGATAG556.184564E-1140.909091