##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546678_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1487414
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432331549924903	31.0	31.0	33.0	30.0	34.0
2	31.841701100029987	31.0	31.0	34.0	30.0	34.0
3	31.955220268197017	33.0	31.0	34.0	30.0	34.0
4	35.700264351417964	37.0	35.0	37.0	33.0	37.0
5	35.615628869971644	37.0	35.0	37.0	33.0	37.0
6	35.67605387605603	37.0	35.0	37.0	33.0	37.0
7	36.003498017364365	37.0	35.0	37.0	35.0	37.0
8	35.979255943536906	37.0	35.0	37.0	35.0	37.0
9	37.6990494912647	39.0	37.0	39.0	35.0	39.0
10	37.21437340242864	39.0	37.0	39.0	34.0	39.0
11	36.93691063819488	39.0	37.0	39.0	33.0	39.0
12	36.06833874092889	37.0	35.0	39.0	32.0	39.0
13	35.700808920717435	37.0	35.0	39.0	31.0	39.0
14	36.68903950077114	38.0	35.0	41.0	31.0	41.0
15	36.98506535503901	38.0	35.0	41.0	32.0	41.0
16	37.12460552341177	38.0	35.0	41.0	33.0	41.0
17	37.06177029394641	38.0	35.0	41.0	32.0	41.0
18	36.99000345566198	38.0	35.0	41.0	32.0	41.0
19	36.90421832791677	38.0	35.0	41.0	32.0	41.0
20	36.75976896815547	38.0	35.0	41.0	32.0	41.0
21	36.62023081670604	38.0	35.0	41.0	32.0	41.0
22	36.489144918630586	38.0	35.0	40.0	31.0	41.0
23	36.4345414255883	38.0	35.0	40.0	31.0	41.0
24	36.310764857665724	37.0	35.0	40.0	31.0	41.0
25	36.208822829420725	37.0	35.0	40.0	31.0	41.0
26	36.05754820110608	37.0	35.0	40.0	31.0	41.0
27	35.960411156544176	37.0	35.0	40.0	31.0	41.0
28	35.92213465786929	36.0	35.0	40.0	30.0	41.0
29	35.97696337401692	36.0	35.0	40.0	31.0	41.0
30	35.97442675677384	36.0	35.0	40.0	31.0	41.0
31	35.849668619496654	36.0	35.0	40.0	30.0	41.0
32	35.68430847094353	37.0	34.0	40.0	30.0	41.0
33	35.54574382115538	37.0	34.0	40.0	30.0	41.0
34	35.42275721487091	36.0	34.0	40.0	29.0	41.0
35	35.30582003396499	36.0	34.0	40.0	29.0	41.0
36	35.12665606213199	36.0	34.0	40.0	27.0	41.0
37	35.04655260741125	36.0	34.0	40.0	27.0	41.0
38	35.000636003157155	36.0	34.0	40.0	27.0	41.0
39	34.95021023064191	36.0	34.0	40.0	27.0	41.0
40	34.75238971799378	36.0	34.0	40.0	26.0	41.0
41	34.74534594941287	36.0	34.0	40.0	26.0	41.0
42	34.680475644306156	36.0	34.0	40.0	26.0	41.0
43	34.54346200855982	35.0	34.0	40.0	26.0	41.0
44	34.413839724515164	35.0	34.0	40.0	25.0	41.0
45	34.29117313673261	35.0	34.0	40.0	24.0	41.0
46	34.289527999601994	35.0	34.0	40.0	25.0	41.0
47	34.271664109656086	35.0	33.0	40.0	25.0	41.0
48	34.20824935088684	35.0	34.0	40.0	24.0	41.0
49	34.167407325734466	35.0	34.0	40.0	24.0	41.0
50	34.02656691412075	35.0	34.0	39.0	24.0	41.0
51	33.66987872912316	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	4.0
12	5.0
13	9.0
14	19.0
15	46.0
16	114.0
17	306.0
18	639.0
19	1201.0
20	2192.0
21	3433.0
22	5331.0
23	8093.0
24	11567.0
25	16877.0
26	22106.0
27	26154.0
28	28552.0
29	32044.0
30	37836.0
31	45736.0
32	57479.0
33	75322.0
34	139547.0
35	246736.0
36	103118.0
37	123278.0
38	179399.0
39	320012.0
40	254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.08634045396911	23.284573091284607	28.472301591890353	14.156784862855936
2	31.122404387749476	26.51057472902635	28.996903350378577	13.370117532845596
3	28.78270609258754	25.669786622957698	31.033659761169385	14.513847523285381
4	25.555427070069257	28.672918232583534	30.3680750618187	15.403579635528509
5	23.288943091835897	32.44476655457055	29.696305130918493	14.569985222675058
6	21.951588461585008	40.5449995764461	26.900782162867902	10.60262979910099
7	86.02964608373996	5.122312953891788	6.98299195785437	1.8650490045138746
8	86.46207444598477	4.272448692831989	6.909508717814945	2.3559681433682886
9	80.52801708199601	6.3968068069817825	9.54340889624543	3.531767214776787
10	50.91628826943945	21.59203826238021	15.29009408275033	12.201579385430014
11	45.35045387498033	20.616116293110057	20.198545932739638	13.83488389916997
12	40.59091819762352	20.755216772196576	24.685460806473518	13.96840422370638
13	22.728507328827078	38.60445040856143	24.3610723040122	14.305969958599288
14	16.42784053397373	41.36924891119755	27.07369972314366	15.129210831685057
15	14.877633261486043	22.661209320337175	47.447449062601265	15.013708355575517
16	16.55215024196357	17.976837652462596	46.46103909200801	19.009973013565826
17	17.045825842704183	19.245011812447643	28.852827793741355	34.85633455110682
18	22.134994023183864	22.91729135264291	34.103887686951985	20.843826937221245
19	28.883552259155827	24.476305857010892	26.164000069920007	20.476141813913276
20	32.15836344151662	21.678093657851814	25.075533778759645	21.08800912187192
21	23.470533422436525	26.901857855311302	27.498463776729277	22.1291449455229
22	23.68775606522461	24.199785668280654	25.1888848699824	26.923573396512335
23	20.626402602100022	28.842407023195964	24.882043600503962	25.649146774200055
24	20.930689102025394	23.005229209890455	37.349856865674255	18.7142248224099
25	18.529609106812224	23.836739468634825	35.01688164828353	22.616769776269418
26	17.443630354427214	32.668308890463585	28.597552530768166	21.290508224341036
27	18.432326171462687	32.673485660347424	28.49455497931309	20.399633188876802
28	16.749203651438	28.27074372030921	37.2635325470918	17.716520081160994
29	17.53862744333454	23.846487931403093	35.93148914828017	22.6833954769822
30	19.63004247640536	29.688506360703883	31.19662716634373	19.484823996547025
31	29.081345207185088	26.373155019382633	24.782004203268222	19.763495570164054
32	29.02924135445814	26.628295820800396	26.965390940249318	17.377071884492146
33	28.278206336635257	27.685701492657728	24.86328621352226	19.172805957184753
34	21.217562830523313	28.065353694398464	27.111281727884773	23.60580174719345
35	21.604341494701544	26.668701518205424	29.72507990377931	22.001877083313726
36	30.382193525138263	24.54743601983039	26.230961924521353	18.83940853051
37	21.793528903183645	31.16724731648351	28.84973517796659	18.189488602366254
38	21.952462461695266	31.167449008816643	24.213635208489364	22.666453320998727
39	21.971892156454086	29.703767747244548	27.817877201639895	20.506462894661475
40	24.710000040338468	25.94496219613369	26.49403595770915	22.851001805818687
41	19.09542333203802	24.543402173167657	28.33898295968708	28.02219153510724
42	23.876271165929595	24.482222165449567	25.20085194841517	26.44065472020567
43	23.562572357124512	24.948467608883607	26.862527850349665	24.626432183642212
44	20.53026258997159	29.30482031230041	28.278945875190093	21.885971222537908
45	18.194799833805515	35.533953559667985	24.202945514833125	22.068301091693368
46	22.93201489296188	31.024112990734253	26.257316389384528	19.78655572691934
47	22.88683581033929	27.72200611262231	27.16224265739061	22.22891541964779
48	24.231384133805385	24.413714002960845	30.363301676601136	20.99160018663264
49	22.29520496647201	23.668460832021214	31.554631057661148	22.48170314384563
50	20.516345818985165	31.73702815759432	27.433451614681587	20.313174408738927
51	20.5143288956538	32.06323189105387	24.90786021914544	22.514578994146888
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1716.0
1	2255.0
2	2794.0
3	7317.0
4	11840.0
5	8852.0
6	5864.0
7	6232.0
8	6600.0
9	7185.0
10	7770.0
11	8063.5
12	8357.0
13	8249.5
14	8142.0
15	8136.0
16	8130.0
17	7695.0
18	7260.0
19	7499.5
20	7739.0
21	7083.0
22	6427.0
23	6844.5
24	7262.0
25	8270.0
26	9867.0
27	10456.0
28	12953.0
29	15450.0
30	18189.0
31	20928.0
32	22170.0
33	23412.0
34	27927.0
35	32442.0
36	35894.0
37	39346.0
38	45671.0
39	51996.0
40	72293.0
41	92590.0
42	113226.0
43	133862.0
44	138104.5
45	142347.0
46	137230.5
47	132114.0
48	123537.5
49	114961.0
50	113623.5
51	112286.0
52	105183.0
53	98080.0
54	88454.0
55	78828.0
56	71592.5
57	64357.0
58	57213.0
59	50069.0
60	47870.0
61	45671.0
62	41647.0
63	37623.0
64	32638.0
65	27653.0
66	24813.5
67	21974.0
68	17966.5
69	13959.0
70	12272.0
71	10585.0
72	8877.5
73	7170.0
74	5938.0
75	3608.5
76	2511.0
77	2015.0
78	1519.0
79	1196.5
80	874.0
81	573.0
82	272.0
83	182.0
84	92.0
85	67.5
86	43.0
87	39.0
88	35.0
89	23.5
90	12.0
91	9.0
92	6.0
93	4.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1487414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.115975486056335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.48862484687102	16.392231798120985
2	6.552026920575685	2.636008258365633
3	2.143803139685626	1.2937407421453997
4	1.0721361726925773	0.8626825987039258
5	0.6818097060242748	0.6857633666269793
6	0.544603348947877	0.6573136570235808
7	0.39928559123985674	0.5622413415721528
8	0.30986298086088687	0.49865569016271544
9	0.2899301594491236	0.5249005182132277
>10	3.8355654749023285	19.148560665231788
>50	1.6048286883019947	22.95006077879372
>100	1.0717950497178015	32.27788082007467
>500	0.004380174676620946	0.5185022085458233
>1k	6.738730271724532E-4	0.18442411888276933
>5k	6.738730271724532E-4	0.8070334375366167
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6394	0.4298735926917456	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5513	0.3706432775273058	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1516	0.10192185901168066	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038119850962811966	0.0
2	0.0	0.0	0.0	0.1404450946407658	0.0
3	0.0	0.0	0.0	0.2055917182438783	0.0
4	0.0	0.0	0.0	0.35269265987815096	0.0
5	0.0	0.0	0.0	0.5642679173384142	0.0
6	0.0	0.0	0.0	0.9456008885219582	0.0
7	0.0	0.0	0.0	1.1413769132198568	0.0
8	0.0	0.0	0.0	1.722250832653182	0.0
9	0.0	0.0	0.0	2.0403196420095546	0.0
10	0.0	0.0	0.0	2.539037551078583	0.0
11	0.0	0.0	0.0	2.9242699073694345	0.0
12	0.0	0.0	0.0	3.232321330846691	0.0
13	0.0	0.0	0.0	3.3680602710476033	0.0
14	0.0	0.0	0.0	3.425004739769829	0.0
15	0.0	0.0	0.0	3.486588132154195	0.0
16	0.0	0.0	0.0	3.615267840695328	0.0
17	0.0	0.0	0.0	3.7538977043378643	0.0
18	0.0	0.0	0.0	3.9850371181123747	0.0
19	0.0	0.0	0.0	4.073714513914754	0.0
20	0.0	0.0	0.0	4.168509910488942	0.0
21	0.0	0.0	0.0	4.286231002262988	0.0
22	0.0	0.0	0.0	4.414977941581832	0.0
23	0.0	0.0	0.0	4.5666505761005345	0.0
24	0.0	0.0	0.0	4.67670735921539	0.0
25	0.0	0.0	0.0	4.769687524791349	0.0
26	0.0	0.0	0.0	4.857221997372621	0.0
27	0.0	0.0	0.0	4.956252932942678	0.0
28	0.0	0.0	0.0	5.052729098959671	0.0
29	0.0	0.0	0.0	5.15337357319482	0.0
30	0.0	0.0	0.0	5.316004824480609	0.0
31	0.0	0.0	0.0	5.432919146922107	0.0
32	0.0	0.0	0.0	5.543110391592388	0.0
33	0.0	0.0	0.0	5.654444559483776	0.0
34	0.0	0.0	0.0	5.773980882256049	0.0
35	0.0	0.0	0.0	5.920543977668625	0.0
36	0.0	0.0	0.0	6.051643994207396	0.0
37	0.0	0.0	0.0	6.175953702197236	0.0
38	0.0	0.0	0.0	6.299860025520803	0.0
39	0.0	0.0	0.0	6.435195581055442	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGATC	20	7.0339575E-4	45.0	44
CAACGAA	35	1.2122473E-7	45.0	17
CTATCCG	40	6.8175723E-9	45.0	29
CCGTGTA	20	7.0339575E-4	45.0	34
ATCCGTA	40	6.8175723E-9	45.0	35
CTAACGG	35	1.2122473E-7	45.0	2
CTACGAT	20	7.0339575E-4	45.0	21
ACGGATA	20	7.0339575E-4	45.0	9
GTACTAA	20	7.0339575E-4	45.0	23
CGTACAA	20	7.0339575E-4	45.0	31
CGTCGAA	25	3.8913604E-5	45.0	29
CCAACGC	40	6.8175723E-9	45.0	20
CCGTTGA	20	7.0339575E-4	45.0	32
TAATCCG	25	3.8913604E-5	45.0	1
ACGTTAG	30	2.1659343E-6	44.999996	1
TGTAGCG	105	0.0	42.857147	1
TGATTCG	100	0.0	42.75	15
TCGTTAG	95	0.0	42.63158	1
CGTTAGG	285	0.0	41.05263	2
TCGATAG	55	6.184564E-11	40.90909	1
>>END_MODULE