FastQCFastQC Report
Thu 26 May 2016
SRR1546677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546677_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2409419
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133860.5555696207259925No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC74750.31024076758753877No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27790.11533900911381541No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT207.034828E-445.043
GTCGTAC406.8212103E-945.033
CTATCGT253.892085E-544.99999626
CACTACG253.892085E-544.99999621
GTACGAC253.892085E-544.99999611
TACGTAG1200.043.1250041
TCACGAC1450.041.8965525
TTACCGG1250.041.3999982
ACGTAGG3500.041.142862
CATACGA5250.040.28571318
CGTTTTT86350.039.9449921
TATAACG850.039.7058831
TTACGAA403.460318E-739.37537
ACATACG5400.039.16666417
GGGCGAT57200.038.6669587
GCCAGTA10350.038.0434825
CATATGC10300.038.0097133
AGGGCGA28950.038.005186
ATCGTAG950.037.8947371
CCAGTAG10400.037.86057726
Produced by FastQC (version 0.11.5)