##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546677_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2409419
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.339687285607027	31.0	31.0	33.0	30.0	34.0
2	31.734598257920272	31.0	31.0	34.0	30.0	34.0
3	31.833379748395775	31.0	31.0	34.0	30.0	34.0
4	35.607779302811174	37.0	35.0	37.0	33.0	37.0
5	35.522922331068195	37.0	35.0	37.0	33.0	37.0
6	35.582340804982444	37.0	35.0	37.0	33.0	37.0
7	35.95051545621579	37.0	35.0	37.0	35.0	37.0
8	35.92355542975298	37.0	35.0	37.0	35.0	37.0
9	37.6194065872312	39.0	37.0	39.0	35.0	39.0
10	37.105988207115495	39.0	37.0	39.0	33.0	39.0
11	36.84753959357007	39.0	37.0	39.0	32.0	39.0
12	36.236330003208245	38.0	35.0	39.0	32.0	39.0
13	36.02312175673887	38.0	35.0	39.0	32.0	39.0
14	37.02876461088752	39.0	35.0	41.0	31.0	41.0
15	37.23417263663979	39.0	35.0	41.0	32.0	41.0
16	37.3253751215542	39.0	35.0	41.0	32.0	41.0
17	37.24922688830793	39.0	35.0	41.0	32.0	41.0
18	37.143232455625196	39.0	35.0	41.0	32.0	41.0
19	37.07375595527387	38.0	35.0	41.0	32.0	41.0
20	36.942465382733346	38.0	35.0	41.0	32.0	41.0
21	36.82710603676654	38.0	35.0	41.0	31.0	41.0
22	36.72190391127488	38.0	35.0	41.0	31.0	41.0
23	36.62849840563223	38.0	35.0	40.0	31.0	41.0
24	36.4914300916528	38.0	35.0	40.0	31.0	41.0
25	36.385762293731396	38.0	35.0	40.0	31.0	41.0
26	36.22316168337678	38.0	35.0	40.0	31.0	41.0
27	36.11913162467798	38.0	35.0	40.0	30.0	41.0
28	36.04686025967256	38.0	35.0	40.0	30.0	41.0
29	36.073538060420375	38.0	35.0	40.0	30.0	41.0
30	36.03103320759071	38.0	35.0	40.0	30.0	41.0
31	35.92972206162565	38.0	35.0	40.0	30.0	41.0
32	35.81769090390671	38.0	35.0	40.0	30.0	41.0
33	35.66581196545723	38.0	35.0	40.0	29.0	41.0
34	35.507770130475436	38.0	34.0	40.0	29.0	41.0
35	35.37492939169152	38.0	34.0	40.0	28.0	41.0
36	35.21853027638613	37.0	34.0	40.0	27.0	41.0
37	35.162733837493604	37.0	34.0	40.0	27.0	41.0
38	35.086646199768495	37.0	34.0	40.0	27.0	41.0
39	35.0038370246105	37.0	34.0	40.0	26.0	41.0
40	34.82977722015141	37.0	34.0	40.0	25.0	41.0
41	34.78272479796997	37.0	34.0	40.0	25.0	41.0
42	34.70059005926325	37.0	34.0	40.0	25.0	41.0
43	34.567322661604315	36.0	34.0	40.0	24.0	41.0
44	34.41580646620617	36.0	34.0	40.0	24.0	41.0
45	34.28893604640787	36.0	33.0	40.0	23.0	41.0
46	34.244415354905065	36.0	33.0	40.0	23.0	41.0
47	34.20780071876249	35.0	33.0	40.0	23.0	41.0
48	34.120250566630375	35.0	33.0	40.0	23.0	41.0
49	34.036957457378726	36.0	33.0	40.0	24.0	41.0
50	33.91396598101036	35.0	33.0	40.0	24.0	41.0
51	33.52601643798775	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	2.0
11	3.0
12	12.0
13	12.0
14	32.0
15	72.0
16	213.0
17	524.0
18	1174.0
19	2366.0
20	4056.0
21	6416.0
22	9893.0
23	14455.0
24	20726.0
25	29210.0
26	38140.0
27	44055.0
28	47341.0
29	53008.0
30	62733.0
31	75285.0
32	92391.0
33	118978.0
34	200562.0
35	314600.0
36	181898.0
37	226520.0
38	323072.0
39	541309.0
40	359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.79411426572132	23.463291357792066	27.06179373533619	14.680800641150418
2	32.30509097836449	26.052131239937925	28.651305563706437	12.991472217991143
3	29.246511295876726	26.24914969127412	30.691216430185037	13.81312258266412
4	25.266132623674004	28.910164649652053	29.87097719408704	15.9527255325869
5	23.157532998619168	32.220755294118625	29.78668301362279	14.835028693639421
6	21.36403008360107	40.59924820049979	27.478491702771496	10.558230013127647
7	85.47230680923492	4.73923381528908	7.7712925813235465	2.017166794152449
8	85.10960526168341	4.355531354239341	7.606896102338364	2.9279672817388755
9	78.94405248734239	6.313762778495563	10.610690793091612	4.13149394107044
10	45.07306533234775	26.387523299185407	15.79621477210896	12.743196596357878
11	39.02982420243221	23.185299028520983	23.08747461524957	14.697402153797242
12	34.42203286352436	22.049174510535526	26.915409897572818	16.613382728367295
13	23.362105138209667	33.17741746039191	27.322561995236192	16.137915406162232
14	19.025541012169324	35.01777814485567	29.01898756505199	16.937693277923017
15	17.001443086486827	23.824540272986976	42.26002202190652	16.913994618619675
16	19.679848129362306	20.848760634825243	39.81312507289101	19.658266162921436
17	19.72766048578516	21.57296012026136	29.501759552821653	29.19761984113182
18	23.124495988451987	22.65388460869612	33.565062780695264	20.65655662215663
19	27.822101510779156	25.140376165374306	26.37079727519373	20.666725048652808
20	29.882349230250117	23.176251204128466	25.910478833278894	21.03092073234253
21	24.42020254675505	27.312186049831933	27.512441796134258	20.755169607278766
22	24.252527269022117	24.172923015880592	26.217440802118684	25.357108912978603
23	21.615086458602676	28.749669526138877	25.79044989684235	23.8447941184161
24	21.85958523610879	24.19355039534427	34.44116610684983	19.505698261697116
25	20.660001436030846	25.574754743778477	31.69291019951283	22.07233362067785
26	19.398618505125096	30.584012162268166	27.826168881377626	22.191200451229115
27	20.177685989858965	29.58862696774617	28.603161177030646	21.630525865364223
28	18.8148263128995	28.216594955049327	32.82741607001522	20.141162662035953
29	19.90554569379589	24.92351890642516	33.128235479175686	22.042699920603265
30	21.777947297668028	27.993719647765708	30.497518281378206	19.73081477318806
31	27.327832975501565	26.112768264880454	26.03457514031391	20.524823619304073
32	28.187666819262237	26.069770347125175	26.05051259245486	19.692050241157723
33	27.20481576678859	26.414542261018113	25.36951854368211	21.011123428511187
34	21.709632073126343	26.071970047550884	29.202268264672938	23.016129614649838
35	22.61989301155175	25.822407808687487	29.95958776783947	21.5981114119213
36	27.46537650778051	26.39752571055512	26.56175617441383	19.575341607250547
37	22.35431031298417	29.754891116904115	28.107149482925138	19.78364908718658
38	22.550083650871848	29.855786810015196	26.02096189994351	21.573167639169444
39	22.398802366877657	29.49437187969382	26.491822302389085	21.615003451039442
40	25.010303313786437	25.677808633533644	27.28869490943667	22.023193143243248
41	20.142947324645487	25.156064594825555	28.124166033388132	26.576822047140823
42	23.404065461424516	25.921352824062566	25.965222321231803	24.70935939328112
43	23.18172970330192	25.198771986109513	28.024017408346165	23.595480902242407
44	21.98061026330414	28.59133259926978	27.613462000590182	21.814595136835894
45	20.109121742627583	31.9295232585117	24.9751911145384	22.98616388432232
46	23.05369053701328	29.342136008722434	26.241886529491133	21.362286924773148
47	22.32405405618533	27.281514755216925	28.11370708042063	22.280724108177115
48	23.032814134859898	25.515404336066084	29.598255845081322	21.853525683992697
49	22.63836219437134	24.72982075761833	29.69454461843291	22.937272429577423
50	20.900889384536274	29.319972989338922	28.532023695339	21.247113930785805
51	20.468752010339422	29.703426427698957	26.344193351177193	23.483628210784428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2461.0
1	3201.5
2	3942.0
3	13203.5
4	22465.0
5	16250.5
6	10036.0
7	10377.5
8	10719.0
9	11792.5
10	12866.0
11	13417.5
12	13969.0
13	14138.0
14	14307.0
15	14108.0
16	13909.0
17	13341.5
18	12774.0
19	12136.5
20	11499.0
21	12729.5
22	13960.0
23	13706.5
24	13453.0
25	13314.5
26	15941.5
27	18707.0
28	22331.5
29	25956.0
30	29876.5
31	33797.0
32	38120.5
33	42444.0
34	51037.0
35	59630.0
36	63711.0
37	67792.0
38	76880.0
39	85968.0
40	111221.5
41	136475.0
42	157787.5
43	179100.0
44	185707.0
45	192314.0
46	199997.5
47	207681.0
48	202979.5
49	198278.0
50	190374.0
51	182470.0
52	171119.5
53	159769.0
54	143045.5
55	126322.0
56	116227.5
57	106133.0
58	98714.5
59	91296.0
60	85403.5
61	79511.0
62	72514.0
63	65517.0
64	60185.0
65	54853.0
66	47514.5
67	40176.0
68	34624.0
69	29072.0
70	25619.0
71	22166.0
72	18225.5
73	14285.0
74	11672.5
75	7174.0
76	5288.0
77	4211.5
78	3135.0
79	2323.5
80	1512.0
81	1060.0
82	608.0
83	500.5
84	393.0
85	229.5
86	66.0
87	68.0
88	70.0
89	45.5
90	21.0
91	14.5
92	8.0
93	7.5
94	7.0
95	3.5
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2409419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.9917420915483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.90400061270697	17.57228173557569
2	7.210123278609293	3.1712634318288844
3	2.4769781903993042	1.6341919658895452
4	1.3503679218883682	1.1878777226747614
5	0.8745524366268069	0.9616465815915937
6	0.64702725510453	0.8537553912276749
7	0.5006997976833628	0.770788257116005
8	0.4228582691054753	0.7439511996356911
9	0.34666942557710956	0.6861478138485293
>10	3.948214138754693	20.24864811574716
>50	1.4573578875799267	22.954205713336854
>100	0.8569481902843342	27.589742101051257
>500	0.0030564332217055605	0.4696318241014166
>1k	7.641083054263901E-4	0.27949374056236914
>5k	1.9102707635659753E-4	0.3140261101313256
>10k+	1.9102707635659753E-4	0.5623482956813276
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13386	0.5555696207259925	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7475	0.31024076758753877	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2779	0.11533900911381541	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.300756323412408E-5	0.0	0.0	0.05113265895222043	0.0
2	8.300756323412408E-5	0.0	0.0	0.1658491113417799	0.0
3	8.300756323412408E-5	0.0	0.0	0.2708121750513298	0.0
4	8.300756323412408E-5	0.0	0.0	0.4633482179728806	0.0
5	8.300756323412408E-5	0.0	0.0	0.7802710944007664	0.0
6	8.300756323412408E-5	0.0	0.0	1.3961042060347328	0.0
7	8.300756323412408E-5	0.0	0.0	1.7045188072311208	0.0
8	8.300756323412408E-5	0.0	0.0	2.4979466004044957	0.0
9	8.300756323412408E-5	0.0	0.0	2.942244582615145	0.0
10	8.300756323412408E-5	0.0	0.0	3.631954425527482	0.0
11	8.300756323412408E-5	0.0	0.0	4.099494525443686	0.0
12	8.300756323412408E-5	0.0	0.0	4.537566940411776	0.0
13	8.300756323412408E-5	0.0	0.0	4.76268345190272	0.0
14	8.300756323412408E-5	0.0	0.0	4.873498548820276	0.0
15	8.300756323412408E-5	0.0	0.0	4.972568075540202	0.0
16	8.300756323412408E-5	0.0	0.0	5.139703804112112	0.0
17	8.300756323412408E-5	0.0	0.0	5.314725251191263	0.0
18	8.300756323412408E-5	0.0	0.0	5.585371411116124	0.0
19	8.300756323412408E-5	0.0	0.0	5.712622005554036	0.0
20	8.300756323412408E-5	0.0	0.0	5.854606442466006	0.0
21	8.300756323412408E-5	0.0	0.0	6.020912095405573	0.0
22	8.300756323412408E-5	0.0	0.0	6.189666471460547	0.0
23	8.300756323412408E-5	0.0	0.0	6.386103869854102	0.0
24	8.300756323412408E-5	0.0	0.0	6.540414929906339	0.0
25	8.300756323412408E-5	0.0	0.0	6.6658393579531	0.0
26	8.300756323412408E-5	0.0	0.0	6.791927846505734	0.0
27	8.300756323412408E-5	0.0	0.0	6.925736038439142	0.0
28	8.300756323412408E-5	0.0	0.0	7.060706336257828	0.0
29	8.300756323412408E-5	0.0	0.0	7.208210776124867	0.0
30	8.300756323412408E-5	0.0	0.0	7.436730597708411	0.0
31	8.300756323412408E-5	0.0	0.0	7.592701809025329	0.0
32	8.300756323412408E-5	0.0	0.0	7.766395135092734	0.0
33	8.300756323412408E-5	0.0	0.0	7.920913714053056	0.0
34	8.300756323412408E-5	0.0	0.0	8.077341466967763	0.0
35	8.300756323412408E-5	0.0	0.0	8.264399010715861	0.0
36	8.300756323412408E-5	0.0	0.0	8.418378040515162	0.0
37	1.2451134485118613E-4	0.0	0.0	8.57194203249829	0.0
38	1.2451134485118613E-4	0.0	0.0	8.74883114975021	0.0
39	1.2451134485118613E-4	0.0	0.0	8.984655636898356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGT	20	7.034828E-4	45.0	43
GTCGTAC	40	6.8212103E-9	45.0	33
CTATCGT	25	3.892085E-5	44.999996	26
CACTACG	25	3.892085E-5	44.999996	21
GTACGAC	25	3.892085E-5	44.999996	11
TACGTAG	120	0.0	43.125004	1
TCACGAC	145	0.0	41.89655	25
TTACCGG	125	0.0	41.399998	2
ACGTAGG	350	0.0	41.14286	2
CATACGA	525	0.0	40.285713	18
CGTTTTT	8635	0.0	39.944992	1
TATAACG	85	0.0	39.705883	1
TTACGAA	40	3.460318E-7	39.375	37
ACATACG	540	0.0	39.166664	17
GGGCGAT	5720	0.0	38.666958	7
GCCAGTA	1035	0.0	38.04348	25
CATATGC	1030	0.0	38.00971	33
AGGGCGA	2895	0.0	38.00518	6
ATCGTAG	95	0.0	37.894737	1
CCAGTAG	1040	0.0	37.860577	26
>>END_MODULE