FastQCFastQC Report
Thu 26 May 2016
SRR1546676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546676_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1891357
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194611.0289437689447312No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC64750.3423467912192146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21350.11288191494255183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAG302.1662472E-645.0000041
TACACGA302.1662472E-645.00000417
ACGCGAG502.1827873E-1145.01
CGAACGC207.034443E-445.043
GTCGAAC502.1827873E-1145.027
ACGTTCA253.891765E-545.043
CTATCCG253.891765E-545.01
TCCCGTC253.891765E-545.019
CGAAGTC207.034443E-445.016
GACGGTC1150.044.99999629
CGTTTTT101450.042.8708721
CGCATCG900.042.521
TATAGCG1600.042.18751
GCGCGAC2050.041.7073179
ACGTATG650.041.5384641
TGATTCG1850.041.35135315
AGGGTAC7550.041.1258246
CGAATAT1100.040.90909214
TACGGGT1650.040.909094
ACTACGG1050.040.7142872
Produced by FastQC (version 0.11.5)