##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546676_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1891357
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.273496753917954	31.0	31.0	33.0	30.0	34.0
2	31.66152027353905	31.0	31.0	34.0	30.0	34.0
3	31.719863040134676	31.0	31.0	34.0	30.0	34.0
4	35.52892658551506	37.0	35.0	37.0	33.0	37.0
5	35.44350379119331	37.0	35.0	37.0	33.0	37.0
6	35.526674234425336	37.0	35.0	37.0	33.0	37.0
7	35.940226514613585	37.0	35.0	37.0	35.0	37.0
8	35.9321952439439	37.0	35.0	37.0	35.0	37.0
9	37.66316988278786	39.0	37.0	39.0	35.0	39.0
10	37.02675803669006	39.0	37.0	39.0	33.0	39.0
11	36.75810542377774	39.0	35.0	39.0	32.0	39.0
12	36.06680018632125	37.0	35.0	39.0	32.0	39.0
13	35.813099800830834	37.0	35.0	39.0	31.0	39.0
14	36.81681089291974	39.0	35.0	41.0	31.0	41.0
15	37.08177567746333	39.0	35.0	41.0	32.0	41.0
16	37.19464490310396	39.0	35.0	41.0	32.0	41.0
17	37.142498745609636	38.0	35.0	41.0	32.0	41.0
18	37.044551081577936	38.0	35.0	40.0	32.0	41.0
19	36.97430363490341	38.0	35.0	41.0	32.0	41.0
20	36.78898854103165	38.0	35.0	40.0	32.0	41.0
21	36.668253534367125	38.0	35.0	40.0	32.0	41.0
22	36.55240655254402	38.0	35.0	40.0	31.0	41.0
23	36.5037695157498	38.0	35.0	40.0	31.0	41.0
24	36.38713685464986	38.0	35.0	40.0	31.0	41.0
25	36.27908004676008	37.0	35.0	40.0	31.0	41.0
26	36.155875384710555	37.0	35.0	40.0	31.0	41.0
27	36.02425295700389	37.0	35.0	40.0	30.0	41.0
28	35.98466392119521	37.0	35.0	40.0	30.0	41.0
29	35.96722617676092	37.0	35.0	40.0	30.0	41.0
30	35.92649774738455	37.0	35.0	40.0	30.0	41.0
31	35.769312192251384	37.0	35.0	40.0	30.0	41.0
32	35.532438349819735	37.0	34.0	40.0	29.0	41.0
33	35.33223024526834	37.0	34.0	40.0	29.0	41.0
34	35.15282572248391	37.0	34.0	40.0	27.0	41.0
35	35.00119543798447	37.0	34.0	40.0	27.0	41.0
36	34.828824489506744	36.0	34.0	40.0	25.0	41.0
37	34.73376046933498	36.0	34.0	40.0	25.0	41.0
38	34.687418610024444	36.0	34.0	40.0	25.0	41.0
39	34.60809619759781	36.0	34.0	40.0	24.0	41.0
40	34.47409241089863	36.0	34.0	40.0	23.0	41.0
41	34.45744351806666	36.0	34.0	40.0	23.0	41.0
42	34.38672656722131	36.0	34.0	40.0	23.0	41.0
43	34.26648327100595	35.0	33.0	40.0	23.0	41.0
44	34.13799668703476	35.0	33.0	40.0	23.0	41.0
45	34.01091650069236	35.0	33.0	40.0	23.0	41.0
46	33.99568087886105	35.0	33.0	40.0	23.0	41.0
47	33.95361478557459	35.0	33.0	40.0	23.0	41.0
48	33.88607174637046	35.0	33.0	40.0	23.0	41.0
49	33.832237911721585	35.0	33.0	40.0	23.0	41.0
50	33.673420723850654	35.0	33.0	40.0	23.0	41.0
51	33.31222714696379	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	1.0
13	6.0
14	22.0
15	27.0
16	114.0
17	334.0
18	741.0
19	1651.0
20	2962.0
21	4894.0
22	7441.0
23	11288.0
24	17314.0
25	25742.0
26	33911.0
27	37917.0
28	39535.0
29	42888.0
30	50132.0
31	60662.0
32	74879.0
33	97353.0
34	165230.0
35	274713.0
36	148928.0
37	167286.0
38	227940.0
39	397153.0
40	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.5578708831807	24.50209029812986	29.16482715848991	14.775211660199528
2	31.72288468015293	26.22159645164821	29.1501815891976	12.905337279001266
3	27.183234048357875	25.728035479288152	33.32665382579809	13.762076646555885
4	24.075782625913565	27.823938050828055	33.32453894214577	14.775740381112609
5	21.717000016390347	32.592101861256225	31.6505556592436	14.04034246310982
6	19.75560404513796	40.94763706693131	28.782879170881014	10.513879717049717
7	85.78671292622175	4.106152355160871	8.450652097938146	1.6564826206792265
8	86.65323363066835	3.086091097555882	7.9724769041487145	2.288198367627053
9	81.51993515766722	5.155874855989642	10.466347706963836	2.8578422793793026
10	44.84705954507795	25.5661940077944	17.8996350239537	11.687111423173944
11	39.64137917907619	23.28872867470287	23.62864334972192	13.441248796499023
12	34.79443595259911	23.207992991275574	26.87387944211484	15.12369161401047
13	20.50617625334614	37.538814724031475	27.786927586912462	14.168081435709917
14	16.030077875303288	40.32342915694922	29.095934823515602	14.550558144231893
15	14.052132939471502	25.870049916541404	46.329011392349514	13.74880575163758
16	15.995552399679172	22.255502266362193	44.24289015770159	17.506055176257046
17	16.00506937611461	22.725270797633655	30.294915238106817	30.974744588144915
18	20.11941690542822	25.608174448292946	35.354404271642004	18.918004374636833
19	25.7528324901116	27.945385244562505	27.29204481226971	19.009737453056193
20	27.810984388457598	26.01856762102554	26.508744779541882	19.661703210974977
21	20.626883237802275	30.696320155317053	29.26227042277053	19.41452618411014
22	21.186904428936472	26.27806384516514	26.90258898769508	25.63244273820331
23	17.75947110989623	32.71862477575625	25.8614317656582	23.660472348689325
24	18.373580450438496	26.684491611049634	37.97606691914853	16.965861019363345
25	16.74104888712179	28.067625519666567	35.404368397928046	19.7869571952836
26	16.260388705040878	35.66397036625026	28.662859523611882	19.412781405096975
27	16.928797683356446	35.49028554630353	29.11893418323458	18.46198258710545
28	15.407720488516974	31.36467626154132	36.46958242151006	16.75802082843165
29	15.94045968053625	27.255721685541122	35.63282870446986	21.17098992945277
30	18.642910883561377	32.778528855208194	30.423182931619998	18.155377329610435
31	26.277376507978133	29.457632800153537	26.001172702985208	18.263817988883112
32	27.251967767058254	28.96502352543703	26.739742946466478	17.04326576103824
33	26.20726811490374	29.96282563260135	24.916078773071398	18.913827479423503
34	19.04743525415879	29.895413716183672	27.701856391997914	23.355294637659625
35	18.522468259561787	30.16469127721525	30.229671077432762	21.0831693857902
36	27.406830122499347	28.592856874720106	25.709583119421666	18.290729883358882
37	19.003392802099235	34.57554549458405	28.579850340258346	17.841211363058378
38	19.038658487001662	35.01538842217519	25.040645420193012	20.905307670630137
39	18.84964076057561	34.67621395643445	27.10006624873041	19.374079034259527
40	22.967795080463393	29.09493025378075	26.599631904500313	21.337642761255545
41	16.799578292199726	28.02871166046389	28.069000194040576	27.10270985329581
42	20.927461076888182	28.423771926717166	25.44131012812494	25.207456868269713
43	20.807547173801666	28.531472376711537	27.401490041277242	23.259490408209555
44	18.77704737920974	32.47742229520921	28.268116489906454	20.4774138356746
45	16.900299626141443	37.84267063277847	24.603128864619425	20.653900876460657
46	21.807358420435698	33.760998055893204	25.738028304545363	18.69361521912574
47	20.442782615867866	30.962161030413615	26.398717957529964	22.196338396188555
48	20.88923455487251	28.244218304635243	30.251137146503808	20.615409993988443
49	20.659452446047997	26.85188465213072	30.88010354470362	21.608559357117667
50	18.625463093429744	34.14506092715442	27.699212787432515	19.530263191983323
51	18.56021893275569	34.52351935673698	25.00537973529059	21.910881975216736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2557.0
1	2460.0
2	2363.0
3	14499.5
4	26636.0
5	17922.0
6	9208.0
7	10614.5
8	12021.0
9	14238.0
10	16455.0
11	18505.5
12	20556.0
13	21505.0
14	22454.0
15	21939.0
16	21424.0
17	19757.0
18	18090.0
19	17078.0
20	16066.0
21	15473.5
22	14881.0
23	14312.0
24	13743.0
25	14499.0
26	17588.0
27	19921.0
28	21779.0
29	23637.0
30	26848.5
31	30060.0
32	32866.0
33	35672.0
34	43367.0
35	51062.0
36	53982.0
37	56902.0
38	64017.5
39	71133.0
40	94806.0
41	118479.0
42	143546.0
43	168613.0
44	171818.5
45	175024.0
46	172845.0
47	170666.0
48	168536.0
49	166406.0
50	162596.0
51	158786.0
52	146247.0
53	133708.0
54	111434.5
55	89161.0
56	76690.0
57	64219.0
58	54636.0
59	45053.0
60	38263.0
61	31473.0
62	26330.5
63	21188.0
64	19332.0
65	17476.0
66	14254.5
67	11033.0
68	9083.0
69	7133.0
70	5645.5
71	4158.0
72	3978.0
73	3798.0
74	3000.5
75	1788.0
76	1373.0
77	1029.0
78	685.0
79	529.5
80	374.0
81	254.0
82	134.0
83	98.5
84	63.0
85	45.0
86	27.0
87	22.5
88	18.0
89	11.0
90	4.0
91	2.5
92	1.0
93	3.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1891357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.398450625303298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3386824136872	19.11342474921663
2	7.786980187384485	3.799805032442307
3	3.138258761629627	2.297059543351377
4	1.7972509225803412	1.7540055158342904
5	1.2063562700332737	1.4716611945465943
6	0.821463563624953	1.2025462918553464
7	0.6214027524383328	1.0612885061655961
8	0.48846171072422523	0.9534167145164952
9	0.3725481014148906	0.8180636812129524
>10	3.692927192742163	20.098971230794405
>50	1.0158968673025464	17.71154192225765
>100	0.7156081960502765	27.658598224237014
>500	0.0032866266218290223	0.4998957159204383
>1k	4.382168829105363E-4	0.173207370290046
>5k	2.1910844145526815E-4	0.3461474452555703
>10k+	2.1910844145526815E-4	1.040366862103267
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19461	1.0289437689447312	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6475	0.3423467912192146	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2135	0.11288191494255183	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06788776523945506	0.0
2	0.0	0.0	0.0	0.2219041672196206	0.0
3	0.0	0.0	0.0	0.3545073722200515	0.0
4	0.0	0.0	0.0	0.5594395981298084	0.0
5	0.0	0.0	0.0	0.9398542950907735	0.0
6	0.0	0.0	0.0	1.6691719225931434	0.0
7	0.0	0.0	0.0	2.0760755372994097	0.0
8	0.0	0.0	0.0	3.1305036542545905	0.0
9	0.0	0.0	0.0	3.641353800472359	0.0
10	0.0	0.0	0.0	4.337256266268081	0.0
11	0.0	0.0	0.0	4.824366843488564	0.0
12	0.0	0.0	0.0	5.2577593759401315	0.0
13	0.0	0.0	0.0	5.488017333586415	0.0
14	0.0	0.0	0.0	5.5901133419021365	0.0
15	0.0	0.0	0.0	5.6872922457262165	0.0
16	0.0	0.0	0.0	5.891431390266353	0.0
17	0.0	0.0	0.0	6.10767824371602	0.0
18	0.0	0.0	0.0	6.46588666232763	0.0
19	0.0	0.0	0.0	6.602138041628312	0.0
20	0.0	0.0	0.0	6.758903792356493	0.0
21	0.0	0.0	0.0	6.9475514141433905	0.0
22	0.0	0.0	0.0	7.15031588430952	0.0
23	0.0	0.0	0.0	7.386284027817065	0.0
24	0.0	0.0	0.0	7.560973417498653	0.0
25	0.0	0.0	0.0	7.7075877266957	0.0
26	0.0	0.0	0.0	7.858537547380003	0.0
27	0.0	0.0	0.0	8.01043906570785	0.0
28	0.0	0.0	0.0	8.166993328070799	0.0
29	0.0	0.0	0.0	8.329152032112393	0.0
30	0.0	0.0	0.0	8.590340163173849	0.0
31	0.0	0.0	0.0	8.786072645196015	0.0
32	0.0	0.0	0.0	8.955686314112038	0.0
33	0.0	0.0	0.0	9.128207948049997	0.0
34	0.0	0.0	0.0	9.296023965861549	0.0
35	0.0	0.0	0.0	9.515760377337541	0.0
36	0.0	0.0	0.0	9.701923010832962	0.0
37	0.0	0.0	0.0	9.889037341971928	0.0
38	0.0	0.0	0.0	10.085034184450635	0.0
39	0.0	0.0	0.0	10.291023852186552	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAG	30	2.1662472E-6	45.000004	1
TACACGA	30	2.1662472E-6	45.000004	17
ACGCGAG	50	2.1827873E-11	45.0	1
CGAACGC	20	7.034443E-4	45.0	43
GTCGAAC	50	2.1827873E-11	45.0	27
ACGTTCA	25	3.891765E-5	45.0	43
CTATCCG	25	3.891765E-5	45.0	1
TCCCGTC	25	3.891765E-5	45.0	19
CGAAGTC	20	7.034443E-4	45.0	16
GACGGTC	115	0.0	44.999996	29
CGTTTTT	10145	0.0	42.870872	1
CGCATCG	90	0.0	42.5	21
TATAGCG	160	0.0	42.1875	1
GCGCGAC	205	0.0	41.707317	9
ACGTATG	65	0.0	41.538464	1
TGATTCG	185	0.0	41.351353	15
AGGGTAC	755	0.0	41.125824	6
CGAATAT	110	0.0	40.909092	14
TACGGGT	165	0.0	40.90909	4
ACTACGG	105	0.0	40.714287	2
>>END_MODULE