FastQCFastQC Report
Thu 26 May 2016
SRR1546675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546675_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences420380
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22650.5387982301727009No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15600.37109282078119793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATTA351.2094642E-745.00000444
TAACCGG351.2094642E-745.0000042
AGTTATA351.2094642E-745.00000420
GGGTTGA351.2094642E-745.0000047
CGCACAG351.2094642E-745.00000428
TATCGAG351.2094642E-745.0000041
TAATCAC207.028176E-445.025
TGATCGA207.028176E-445.015
GTTGACG207.028176E-445.01
TATCACG253.88657E-545.01
AATCACG207.028176E-445.01
CGAGTCG207.028176E-445.016
CGAGTCA207.028176E-445.011
CACGGCA207.028176E-445.040
CGAAACG207.028176E-445.040
CCCTAAA207.028176E-445.030
AGCTTAG253.88657E-545.01
TAAACGC207.028176E-445.035
GTGATAG406.7975634E-945.01
CTAAGCG253.88657E-545.01