##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546675_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	420380
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.423240877301488	31.0	31.0	33.0	30.0	34.0
2	31.827689233550597	31.0	31.0	34.0	30.0	34.0
3	31.94195727674961	31.0	31.0	34.0	30.0	34.0
4	35.67804367477044	37.0	35.0	37.0	33.0	37.0
5	35.58134069175508	37.0	35.0	37.0	33.0	37.0
6	35.6546124934583	37.0	35.0	37.0	33.0	37.0
7	35.98498501355916	37.0	35.0	37.0	35.0	37.0
8	35.97737523193301	37.0	35.0	37.0	35.0	37.0
9	37.717833864598695	39.0	38.0	39.0	35.0	39.0
10	37.2276392787478	39.0	37.0	39.0	34.0	39.0
11	36.911732242257	39.0	37.0	39.0	33.0	39.0
12	35.750299728816785	37.0	35.0	39.0	32.0	39.0
13	35.264263285598744	37.0	35.0	39.0	30.0	39.0
14	36.19097958989486	38.0	35.0	41.0	31.0	41.0
15	36.5664089633189	38.0	35.0	41.0	32.0	41.0
16	36.77032446833817	38.0	35.0	41.0	32.0	41.0
17	36.73442123792759	38.0	35.0	41.0	32.0	41.0
18	36.68255625862315	37.0	35.0	40.0	32.0	41.0
19	36.60496931347828	37.0	35.0	40.0	32.0	41.0
20	36.42198249203102	36.0	35.0	40.0	32.0	41.0
21	36.26959655549741	36.0	35.0	40.0	31.0	41.0
22	36.17442789856796	36.0	35.0	40.0	31.0	41.0
23	36.16143251344022	36.0	35.0	40.0	31.0	41.0
24	36.01785051619963	36.0	35.0	40.0	31.0	41.0
25	35.906927065987915	35.0	35.0	40.0	31.0	41.0
26	35.760863980208384	35.0	35.0	40.0	31.0	41.0
27	35.68929064180028	35.0	34.0	40.0	31.0	41.0
28	35.67713735191969	36.0	35.0	40.0	30.0	41.0
29	35.71129216423236	36.0	35.0	40.0	30.0	41.0
30	35.72317902849802	36.0	35.0	40.0	31.0	41.0
31	35.602697559351064	36.0	35.0	40.0	30.0	41.0
32	35.395970312574335	35.0	34.0	40.0	30.0	41.0
33	35.278792996812406	35.0	34.0	40.0	30.0	41.0
34	35.16147057424235	35.0	34.0	40.0	29.0	41.0
35	35.076250059470006	35.0	34.0	40.0	29.0	41.0
36	34.855445073504924	35.0	34.0	40.0	27.0	41.0
37	34.75627051715115	35.0	34.0	40.0	27.0	41.0
38	34.75246681573814	35.0	34.0	40.0	27.0	41.0
39	34.728938103620536	35.0	34.0	40.0	27.0	41.0
40	34.53781102811742	35.0	34.0	40.0	26.0	41.0
41	34.56198677387126	35.0	34.0	40.0	26.0	41.0
42	34.498891479137924	35.0	34.0	40.0	26.0	41.0
43	34.38382178029402	35.0	34.0	40.0	26.0	41.0
44	34.24067272467767	35.0	34.0	40.0	26.0	41.0
45	34.10670107997526	35.0	34.0	40.0	24.0	41.0
46	34.09378181645178	35.0	34.0	40.0	25.0	41.0
47	34.10348732099529	35.0	33.0	40.0	25.0	41.0
48	34.095473143346496	35.0	34.0	39.0	25.0	41.0
49	34.05521908749227	35.0	34.0	39.0	25.0	41.0
50	33.92737285313288	35.0	34.0	39.0	24.0	41.0
51	33.556294305152484	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	3.0
14	2.0
15	10.0
16	29.0
17	73.0
18	191.0
19	384.0
20	602.0
21	1019.0
22	1586.0
23	2374.0
24	3493.0
25	4862.0
26	6555.0
27	7744.0
28	8267.0
29	9251.0
30	10958.0
31	13265.0
32	16792.0
33	22857.0
34	43984.0
35	81368.0
36	26190.0
37	30414.0
38	44915.0
39	83107.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.093819877253914	24.685522622389268	29.754269946239116	13.466387554117704
2	30.049716922784146	27.028640753603884	29.61035253817974	13.311289785432228
3	28.312954945525476	25.63347447547457	31.77934249964318	14.27422807935677
4	25.60231219372948	28.264903182834576	30.275227175412724	15.857557448023218
5	22.725867072648555	32.29102240829725	30.212902611922548	14.770207907131644
6	21.11660878253009	40.934630572339316	27.253675246205816	10.695085398924782
7	86.2676625909891	5.305913697131167	6.589038488986156	1.8373852228935723
8	87.78724011608544	3.8943336980826873	5.936533612445882	2.381892573385984
9	82.90689376278605	5.2709453351729385	8.628145963176175	3.1940149388648367
10	56.97369047052666	19.36367096436557	13.045815690565679	10.61682287454208
11	52.91854988343879	18.367905228602694	17.337884770921548	11.375660117036967
12	45.77096912317427	20.677482277939006	20.959370093724726	12.592178505161996
13	20.931538132166136	45.96174889385794	20.820448165945095	12.286264808030829
14	14.804462629049908	47.153527760597555	25.19839193110995	12.84361767924259
15	12.090013797040774	21.895427946143965	53.89290641800276	12.121651838812502
16	13.790618012274608	15.856130167943288	51.05666301917313	19.296588800608973
17	14.162424473095768	16.951329749274464	28.28559874399353	40.600647033636236
18	21.044055378467103	21.834292782720397	35.67391407773919	21.447737761073316
19	30.058280603263714	24.269470479090348	25.513820828773966	20.158428088871975
20	33.10243113373615	21.623768970931064	24.198582235120604	21.07521766021219
21	21.412769399115085	27.700651791236503	28.0382035301394	22.848375279509014
22	22.770350635139636	24.378419525191493	23.836290974832295	29.014938864836576
23	19.17526999381512	30.392739901993433	23.05223845092535	27.379751653266094
24	19.490698891479138	22.274846567391407	40.90418193063419	17.330272610495268
25	15.724582520576622	24.57086445596841	38.23588182120938	21.468671202245588
26	15.995765735762882	35.57424235215757	27.744183833674295	20.685808078405252
27	17.462771777915222	35.78429040392026	27.326228650268803	19.426709167895716
28	13.900280698415719	29.43027736809553	39.447166849041345	17.222275084447407
29	13.84318949521861	24.455968409534233	39.5249536133974	22.175888481849753
30	17.90903468290594	31.560730767400923	32.1808839621295	18.349350587563634
31	31.143251344022076	27.625243827013655	23.454493553451638	17.77701127551263
32	31.678719254008282	26.364003996384223	25.679861078072218	16.277415671535277
33	29.636043579618445	28.37504162900233	22.80436747704458	19.184547314334647
34	20.49431466768162	28.451876873305103	26.441077120700317	24.612731338312955
35	20.400114182406394	26.40967695894191	30.243351253627672	22.946857605024025
36	32.87739664113421	23.883391217469907	25.74099624149579	17.498215899900092
37	20.90394405062087	32.88596032161378	28.573671440125604	17.636424187639754
38	21.368999476663973	33.36742946857605	22.92354536371854	22.340025691041436
39	21.091155621104715	32.73609591322137	26.42823159998097	19.744516865692944
40	25.347780579475714	25.548313430705555	24.977163518721156	24.12674247109758
41	17.053618155002617	23.879823017270088	27.0243589133641	32.042199914363195
42	23.810599933393597	24.593938817260575	23.732099528997573	27.863361720348255
43	22.882154241400638	25.034492601931586	26.58404300870641	25.499310147961367
44	19.107712070031877	31.780056139683143	27.941386364717637	21.170845425567343
45	16.676578333888386	39.54017793424997	21.74366049764499	22.03958323421666
46	22.49631285979352	33.79347257243446	25.31376373757077	18.396450830201246
47	22.258670726485562	28.393834150054715	25.888481849754985	23.45901327370474
48	24.66173462105714	23.140491935867548	30.17198724963129	22.025786193444027
49	21.51172748465674	22.419715495504068	32.93210904419811	23.136447975641087
50	19.066320947713976	34.45192444930777	26.45487416147295	20.026880441505305
51	18.30819734525905	34.57443265616823	23.338645986964178	23.778724011608546
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	493.0
1	562.5
2	632.0
3	1639.5
4	2647.0
5	2001.5
6	1356.0
7	1560.0
8	1764.0
9	1972.0
10	2180.0
11	2266.5
12	2353.0
13	2329.5
14	2306.0
15	2180.5
16	2055.0
17	2056.5
18	2058.0
19	1872.0
20	1686.0
21	1639.5
22	1593.0
23	1611.5
24	1630.0
25	1606.0
26	1875.5
27	2169.0
28	2412.5
29	2656.0
30	3670.0
31	4684.0
32	5438.5
33	6193.0
34	7722.5
35	9252.0
36	10398.5
37	11545.0
38	12982.0
39	14419.0
40	22215.0
41	30011.0
42	38069.5
43	46128.0
44	45924.5
45	45721.0
46	43377.0
47	41033.0
48	38934.5
49	36836.0
50	34894.5
51	32953.0
52	30934.0
53	28915.0
54	25052.0
55	21189.0
56	17709.0
57	14229.0
58	12692.5
59	11156.0
60	10339.5
61	9523.0
62	8901.0
63	8279.0
64	7241.0
65	6203.0
66	5317.0
67	4431.0
68	3880.0
69	3329.0
70	2628.5
71	1928.0
72	1701.5
73	1475.0
74	1157.5
75	675.5
76	511.0
77	363.0
78	215.0
79	171.0
80	127.0
81	91.5
82	56.0
83	41.0
84	26.0
85	15.5
86	5.0
87	3.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	420380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.068528204444373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77883808459002	18.960906863523114
2	5.731431279802589	2.758942308195266
3	2.0149714088321793	1.4549218855387895
4	1.235803630275324	1.1897589812174554
5	0.9018497182292504	1.0853097689685463
6	0.6848855892524178	0.9890512873043593
7	0.5709046172513271	0.9618583717652762
8	0.5319111313813477	1.0241854454327908
9	0.4709213211744121	1.020094479068449
>10	7.558737129651581	43.53630400566429
>50	1.4057651328425929	22.675504263580766
>100	0.11198129080965177	3.4226949223257344
>500	0.0	0.0
>1k	0.0019996659073152102	0.9204674174151679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2265	0.5387982301727009	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1560	0.37109282078119793	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03806080213140492	0.0
2	0.0	0.0	0.0	0.16247204909843474	0.0
3	0.0	0.0	0.0	0.24144821352109996	0.0
4	0.0	0.0	0.0	0.4208097435653456	0.0
5	0.0	0.0	0.0	0.6919929587516057	0.0
6	0.0	0.0	0.0	1.1622817450877778	0.0
7	0.0	0.0	0.0	1.3739949569437175	0.0
8	0.0	0.0	0.0	1.989628431419192	0.0
9	0.0	0.0	0.0	2.283410247870974	0.0
10	0.0	0.0	0.0	2.7506065940339695	0.0
11	0.0	0.0	0.0	3.1250297350016654	0.0
12	0.0	0.0	0.0	3.4437889528521812	0.0
13	0.0	0.0	0.0	3.609591322137114	0.0
14	0.0	0.0	0.0	3.6752462058137874	0.0
15	0.0	0.0	0.0	3.7413768495171036	0.0
16	0.0	0.0	0.0	3.8945715780960084	0.0
17	0.0	0.0	0.0	4.05085874684809	0.0
18	0.0	0.0	0.0	4.309672201341643	0.0
19	0.0	0.0	0.0	4.40934392692326	0.0
20	0.0	0.0	0.0	4.5071126123983065	0.0
21	0.0	0.0	0.0	4.639136019791617	0.0
22	0.0	0.0	0.0	4.765212426851896	0.0
23	0.0	0.0	0.0	4.922926875683905	0.0
24	0.0	0.0	0.0	5.032827441838337	0.0
25	0.0	0.0	0.0	5.119653646700605	0.0
26	0.0	0.0	0.0	5.213854131975832	0.0
27	0.0	0.0	0.0	5.304248537037918	0.0
28	0.0	0.0	0.0	5.3932156620200775	0.0
29	0.0	0.0	0.0	5.483847947095485	0.0
30	0.0	0.0	0.0	5.637042675674389	0.0
31	0.0	0.0	0.0	5.751700842095247	0.0
32	0.0	0.0	0.0	5.856368047956611	0.0
33	0.0	0.0	0.0	5.948665493125268	0.0
34	0.0	0.0	0.0	6.060231219372948	0.0
35	0.0	0.0	0.0	6.213663827965174	0.0
36	0.0	0.0	0.0	6.335934154812312	0.0
37	0.0	0.0	0.0	6.460821161805985	0.0
38	0.0	0.0	0.0	6.565012607640706	0.0
39	0.0	0.0	0.0	6.693229934820876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGATTA	35	1.2094642E-7	45.000004	44
TAACCGG	35	1.2094642E-7	45.000004	2
AGTTATA	35	1.2094642E-7	45.000004	20
GGGTTGA	35	1.2094642E-7	45.000004	7
CGCACAG	35	1.2094642E-7	45.000004	28
TATCGAG	35	1.2094642E-7	45.000004	1
TAATCAC	20	7.028176E-4	45.0	25
TGATCGA	20	7.028176E-4	45.0	15
GTTGACG	20	7.028176E-4	45.0	1
TATCACG	25	3.88657E-5	45.0	1
AATCACG	20	7.028176E-4	45.0	1
CGAGTCG	20	7.028176E-4	45.0	16
CGAGTCA	20	7.028176E-4	45.0	11
CACGGCA	20	7.028176E-4	45.0	40
CGAAACG	20	7.028176E-4	45.0	40
CCCTAAA	20	7.028176E-4	45.0	30
AGCTTAG	25	3.88657E-5	45.0	1
TAAACGC	20	7.028176E-4	45.0	35
GTGATAG	40	6.7975634E-9	45.0	1
CTAAGCG	25	3.88657E-5	45.0	1
>>END_MODULE