FastQCFastQC Report
Thu 26 May 2016
SRR1546674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546674_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1742710
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92850.5327908831647261No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21800.12509252830361908No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAAC207.034289E-445.00000413
TCGAAAC253.8916376E-545.025
CGGTCTA1000.040.531
CGTTTTT63500.039.4370081
TGATTCG3500.039.21428715
GTCGTTT1150.039.1304329
CACGACC10100.038.98514627
TATACGG1350.038.3333362
AGGGCGA22750.037.8791246
ACGACCA10400.037.6442328
CACGTGA9400.037.5797943
GACACGA10550.037.5355525
ACACGAC10500.037.526
TAATGCG900.037.51
GTACGGG4150.037.409643
CGTAAGG2850.037.1052632
CGAGACA10700.037.00934622
AACACGT9600.036.7968741
GGGCGAT45050.036.759167
AGGGTAC7300.036.678086
Produced by FastQC (version 0.11.5)