##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546674_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1742710
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334932375438253	31.0	31.0	33.0	30.0	34.0
2	31.739000751702807	31.0	31.0	34.0	30.0	34.0
3	31.85596685621819	31.0	31.0	34.0	30.0	34.0
4	35.61987364507004	37.0	35.0	37.0	33.0	37.0
5	35.545779848626566	37.0	35.0	37.0	33.0	37.0
6	35.6091369189366	37.0	35.0	37.0	33.0	37.0
7	35.95230531758009	37.0	35.0	37.0	35.0	37.0
8	35.92366314533112	37.0	35.0	37.0	35.0	37.0
9	37.638161254597726	39.0	37.0	39.0	35.0	39.0
10	37.076016089883	39.0	37.0	39.0	33.0	39.0
11	36.85556690441898	39.0	37.0	39.0	32.0	39.0
12	36.41821702979842	39.0	35.0	39.0	32.0	39.0
13	36.298074837465784	39.0	35.0	39.0	32.0	39.0
14	37.364987289910545	39.0	35.0	41.0	32.0	41.0
15	37.48836467341095	39.0	35.0	41.0	32.0	41.0
16	37.527477893625445	39.0	35.0	41.0	32.0	41.0
17	37.424216306786555	39.0	35.0	41.0	32.0	41.0
18	37.31632285348681	39.0	35.0	41.0	32.0	41.0
19	37.27606945504416	39.0	35.0	41.0	32.0	41.0
20	37.18038170435701	39.0	35.0	41.0	32.0	41.0
21	37.073328895800216	39.0	35.0	41.0	32.0	41.0
22	36.95237761876617	39.0	35.0	41.0	31.0	41.0
23	36.870756465504876	39.0	35.0	41.0	31.0	41.0
24	36.74059711598602	38.0	35.0	41.0	31.0	41.0
25	36.63419616574186	38.0	35.0	41.0	31.0	41.0
26	36.48663174022069	38.0	35.0	40.0	31.0	41.0
27	36.37552145795915	38.0	35.0	40.0	30.0	41.0
28	36.264822030056635	38.0	35.0	40.0	30.0	41.0
29	36.30307911241687	38.0	35.0	40.0	30.0	41.0
30	36.26456553299172	38.0	35.0	40.0	30.0	41.0
31	36.189138755157195	38.0	35.0	40.0	30.0	41.0
32	36.11690355825123	38.0	35.0	40.0	30.0	41.0
33	35.983106196670704	38.0	35.0	41.0	30.0	41.0
34	35.859040230445686	38.0	35.0	41.0	29.0	41.0
35	35.73910576056831	38.0	35.0	41.0	29.0	41.0
36	35.61708660649219	38.0	35.0	40.0	28.0	41.0
37	35.564416913886994	38.0	34.0	40.0	28.0	41.0
38	35.48217775763036	38.0	34.0	40.0	28.0	41.0
39	35.39302465699973	38.0	34.0	40.0	27.0	41.0
40	35.25425113759604	38.0	34.0	40.0	27.0	41.0
41	35.190336315279076	38.0	34.0	40.0	26.0	41.0
42	35.11774879354569	37.0	34.0	40.0	26.0	41.0
43	34.983804534317244	37.0	34.0	40.0	26.0	41.0
44	34.83820142192333	37.0	34.0	40.0	26.0	41.0
45	34.704778764108774	37.0	34.0	40.0	25.0	41.0
46	34.63889861193199	36.0	34.0	40.0	25.0	41.0
47	34.582878390552644	36.0	33.0	40.0	25.0	41.0
48	34.487540095598234	36.0	33.0	40.0	24.0	41.0
49	34.39831985815196	36.0	33.0	40.0	24.0	41.0
50	34.3041446941832	36.0	33.0	40.0	24.0	41.0
51	33.93845964044505	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	4.0
13	9.0
14	15.0
15	60.0
16	136.0
17	365.0
18	772.0
19	1456.0
20	2549.0
21	4179.0
22	6065.0
23	9182.0
24	13212.0
25	18480.0
26	24609.0
27	29516.0
28	32795.0
29	37254.0
30	43853.0
31	52329.0
32	64837.0
33	82146.0
34	132270.0
35	194470.0
36	138760.0
37	174612.0
38	252098.0
39	426406.0
40	266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.59868824990962	22.48056188350328	26.797401747852483	14.123348118734613
2	32.99435935984759	24.54740031330514	27.7491378370469	14.709102489800369
3	30.332528074091503	25.297496427977116	30.23193761440515	14.138037883526234
4	25.830861130079015	28.77627373458579	29.702532262969743	15.690332872365452
5	23.11027078515645	32.2495423793976	29.071618341548504	15.568568493897436
6	21.410446947570165	41.51648868715965	26.47284975698768	10.600214608282503
7	86.8623006696467	4.161048022906852	7.120748718949223	1.8559025884972256
8	87.0937218470084	3.430461752098743	7.003058454935129	2.4727579459577322
9	80.99597752925042	5.557665934091157	9.504622111538925	3.9417344251194972
10	41.7517544514004	26.978728531999014	16.580326043920103	14.68919097268048
11	34.52054558704546	25.874528751197843	23.11130365924336	16.493622002513327
12	30.59562405678512	23.748300061398623	27.77082819287202	17.88524768894423
13	23.063160250414587	29.902049107424645	29.150633209197167	17.8841574329636
14	20.02232155665601	31.207659335173375	30.638890004647934	18.131129103522674
15	19.98198208537278	23.901394953836267	37.94922850043897	18.16739446035198
16	22.92917352858479	21.942090192860544	35.75029695129999	19.37843932725468
17	22.168117472212817	22.83030452570996	29.232115498275675	25.76946250380155
18	24.958713727470432	23.148716653947012	31.171795651600092	20.72077396698246
19	27.67396755627729	25.051041194461497	26.787818971601702	20.487172277659507
20	29.6724641506619	24.36291752500416	25.981660746767965	19.982957577565973
21	26.382415892489284	25.81066270349054	28.02858765944994	19.77833374457024
22	25.52794211314562	23.78967240676877	27.534816464012945	23.14756901607267
23	23.334461843909775	27.44547285549518	26.121557803650635	23.098507496944414
24	23.130526593638642	24.967493157209177	32.08778282100866	19.814197428143522
25	22.34611610652375	26.11599175996006	29.593793574375542	21.94409855914065
26	21.344170860326734	28.92477807552605	27.30637914512455	22.42467191902267
27	21.747565573159044	28.06932880398919	28.252606572522104	21.930499050329658
28	20.49509097899249	27.73743193072858	31.28724802175921	20.48022906851972
29	21.458934647761247	25.943042732296252	30.774139128139506	21.823883491803
30	23.225436245846986	26.11627866942865	30.328052286381556	20.330232798342813
31	26.379374652122266	26.20470416764694	26.879916911017897	20.536004269212892
32	27.04339792621836	25.501833351504267	26.940626954570757	20.51414176770662
33	25.38259377635981	26.70295115079388	26.014081516718214	21.9003735561281
34	22.151591486822248	27.773525141876732	28.322382955282286	21.75250041601873
35	21.57696920313764	27.90418371387092	28.979233492663724	21.539613590327708
36	25.31494052366716	28.359222131048767	26.017696576022402	20.30814076926167
37	22.520384917743055	29.4879239804672	27.314986429182138	20.676704672607606
38	22.347435890079247	30.465654067515537	25.424826850135705	21.76208319226951
39	22.54873157323938	28.71585060050152	25.981603364874246	22.753814461384856
40	24.555663306000426	26.094817841178397	26.38442425876939	22.965094594051795
41	20.304870001319784	25.792415261288454	27.548358590930217	26.354356146461544
42	23.36716952332861	26.347699846790345	25.97959499859414	24.305535631286904
43	22.878964371582192	25.47015854617234	27.755966282399253	23.894910799846215
44	22.425876938790733	27.842326032443722	27.60901125258936	22.122785776176183
45	21.34090009238485	29.624779796982857	26.367381836335362	22.66693827429693
46	23.55182445731074	28.193675367674487	26.595933919011195	21.65856625600358
47	22.243689426238443	27.88335408645156	27.911585978160453	21.961370509149543
48	23.685007832628493	25.681840352095296	28.685610342512526	21.947541472763685
49	22.86387293353455	25.078010684508612	28.926155240975266	23.131961140981574
50	21.844770501116077	28.44913955850371	28.180018476969774	21.526071463410435
51	21.076656471816882	28.890865376339146	27.025380011591142	23.007098140252825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1615.0
1	2031.0
2	2447.0
3	9452.0
4	16457.0
5	11582.5
6	6708.0
7	6845.5
8	6983.0
9	7545.5
10	8108.0
11	8133.0
12	8158.0
13	8307.5
14	8457.0
15	8453.0
16	8449.0
17	8069.5
18	7690.0
19	7595.0
20	7500.0
21	8293.0
22	9086.0
23	9156.5
24	9227.0
25	10550.5
26	13806.5
27	15739.0
28	18103.5
29	20468.0
30	23491.5
31	26515.0
32	31028.0
33	35541.0
34	40020.5
35	44500.0
36	49006.5
37	53513.0
38	59942.5
39	66372.0
40	77100.0
41	87828.0
42	100979.0
43	114130.0
44	122551.5
45	130973.0
46	132925.5
47	134878.0
48	135463.0
49	136048.0
50	132575.5
51	129103.0
52	121118.5
53	113134.0
54	105689.0
55	98244.0
56	89543.0
57	80842.0
58	76745.5
59	72649.0
60	68071.0
61	63493.0
62	60240.5
63	56988.0
64	50700.0
65	44412.0
66	38051.0
67	31690.0
68	28333.0
69	24976.0
70	21295.5
71	17615.0
72	15123.0
73	12631.0
74	10121.5
75	6338.5
76	5065.0
77	3901.0
78	2737.0
79	1933.5
80	1130.0
81	837.0
82	544.0
83	419.5
84	295.0
85	237.5
86	180.0
87	112.5
88	45.0
89	30.0
90	15.0
91	17.0
92	19.0
93	18.5
94	18.0
95	13.5
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1742710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.628162539139197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2062369788737	18.478696789112664
2	6.123156194722201	2.8935785964286644
3	2.304185005011779	1.6333097345599672
4	1.4045575961232524	1.3274846070713133
5	0.9684992932515566	1.144192935999461
6	0.7567363729807512	1.0728174012040712
7	0.6436082329809745	1.0645095958281845
8	0.5563935697279737	1.0517246161011553
9	0.48352727280895036	1.0282374894632922
>10	6.835281184617517	38.88971921667429
>50	1.5072417267623623	24.203803257041496
>100	0.20910743333596066	6.379391101041974
>500	7.345694014981243E-4	0.10593265889479245
>1k	4.897129343320828E-4	0.18941754517834544
>5k	2.448564671660414E-4	0.5371844554004085
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9285	0.5327908831647261	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2180	0.12509252830361908	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1476378743451291E-4	0.0	0.0	0.042060928094748984	0.0
2	1.1476378743451291E-4	0.0	0.0	0.1377165449214155	0.0
3	1.1476378743451291E-4	0.0	0.0	0.2291832835067223	0.0
4	1.1476378743451291E-4	0.0	0.0	0.355308685897252	0.0
5	1.1476378743451291E-4	0.0	0.0	0.5835164772107809	0.0
6	1.1476378743451291E-4	0.0	0.0	1.0721806841069368	0.0
7	1.1476378743451291E-4	0.0	0.0	1.3102581611398338	0.0
8	1.1476378743451291E-4	0.0	0.0	1.914202592513958	0.0
9	1.1476378743451291E-4	0.0	0.0	2.2476487769049354	0.0
10	1.1476378743451291E-4	0.0	0.0	2.7278778454246546	0.0
11	1.1476378743451291E-4	0.0	0.0	3.0571925334679895	0.0
12	1.1476378743451291E-4	0.0	0.0	3.3513895025563634	0.0
13	1.1476378743451291E-4	0.0	0.0	3.5095340016411223	0.0
14	1.1476378743451291E-4	0.0	0.0	3.5948608775986823	0.0
15	1.1476378743451291E-4	0.0	0.0	3.673933127141062	0.0
16	1.7214568115176936E-4	0.0	0.0	3.8056819551158827	0.0
17	1.7214568115176936E-4	0.0	0.0	3.9445461379116433	0.0
18	1.7214568115176936E-4	0.0	0.0	4.1603020582885275	0.0
19	1.7214568115176936E-4	0.0	0.0	4.262269683424092	0.0
20	1.7214568115176936E-4	0.0	0.0	4.370893608230859	0.0
21	1.7214568115176936E-4	0.0	0.0	4.4936908607857875	0.0
22	1.7214568115176936E-4	0.0	0.0	4.6252101611857395	0.0
23	1.7214568115176936E-4	0.0	0.0	4.76763202139197	0.0
24	1.7214568115176936E-4	0.0	0.0	4.89019974637203	0.0
25	1.7214568115176936E-4	0.0	0.0	4.996872686792409	0.0
26	1.7214568115176936E-4	0.0	0.0	5.101135587676665	0.0
27	1.7214568115176936E-4	0.0	0.0	5.218309414647302	0.0
28	1.7214568115176936E-4	0.0	0.0	5.326244756729461	0.0
29	1.7214568115176936E-4	0.0	0.0	5.448410808453501	0.0
30	1.7214568115176936E-4	0.0	0.0	5.62514704110265	0.0
31	1.7214568115176936E-4	0.0	0.0	5.763150495492653	0.0
32	1.7214568115176936E-4	0.0	0.0	5.915327277630817	0.0
33	1.7214568115176936E-4	0.0	0.0	6.045297266900402	0.0
34	1.7214568115176936E-4	0.0	0.0	6.17314412610245	0.0
35	1.7214568115176936E-4	0.0	0.0	6.314016675178315	0.0
36	1.7214568115176936E-4	0.0	0.0	6.4518479838871645	0.0
37	1.7214568115176936E-4	0.0	0.0	6.593868170837374	0.0
38	1.7214568115176936E-4	0.0	0.0	6.742142984202765	0.0
39	1.7214568115176936E-4	0.0	0.0	6.939020261546672	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGAAC	20	7.034289E-4	45.000004	13
TCGAAAC	25	3.8916376E-5	45.0	25
CGGTCTA	100	0.0	40.5	31
CGTTTTT	6350	0.0	39.437008	1
TGATTCG	350	0.0	39.214287	15
GTCGTTT	115	0.0	39.130432	9
CACGACC	1010	0.0	38.985146	27
TATACGG	135	0.0	38.333336	2
AGGGCGA	2275	0.0	37.879124	6
ACGACCA	1040	0.0	37.64423	28
CACGTGA	940	0.0	37.57979	43
GACACGA	1055	0.0	37.53555	25
ACACGAC	1050	0.0	37.5	26
TAATGCG	90	0.0	37.5	1
GTACGGG	415	0.0	37.40964	3
CGTAAGG	285	0.0	37.105263	2
CGAGACA	1070	0.0	37.009346	22
AACACGT	960	0.0	36.79687	41
GGGCGAT	4505	0.0	36.75916	7
AGGGTAC	730	0.0	36.67808	6
>>END_MODULE