FastQCFastQC Report
Thu 26 May 2016
SRR1546673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546673_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences966082
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53730.5561639695181154No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19190.19863738274804832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACG302.1651413E-645.0000041
CTTAACG207.032729E-445.01
GTCGCAA207.032729E-445.09
ACGCATG207.032729E-445.01
CGATCGA207.032729E-445.010
AACGGGC1100.040.9090924
TACGGGT1600.040.781254
CGTTTTT34200.040.1315771
GTACGAG800.039.3751
GACCGAT8000.039.093759
ACGGGTA1100.038.8636365
TCACGAC356.247252E-638.5714325
TGATTCG700.038.5714315
CGGGATC1400.038.571436
ACGTAAG356.247252E-638.571431
AGGGCGA11850.038.5443046
TAGGGCG5350.038.2710275
TTGGGCG4100.037.8658525
TACGCGG301.1397869E-437.5000042
AGTTGCG301.1397869E-437.5000041
Produced by FastQC (version 0.11.5)