##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546673_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 966082 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36681254800317 31.0 31.0 33.0 30.0 34.0 2 31.77461540531756 31.0 31.0 34.0 30.0 34.0 3 31.886182539370363 31.0 31.0 34.0 30.0 34.0 4 35.65283795785451 37.0 35.0 37.0 33.0 37.0 5 35.56595506385586 37.0 35.0 37.0 33.0 37.0 6 35.63644493945649 37.0 35.0 37.0 33.0 37.0 7 35.987193633666706 37.0 35.0 37.0 35.0 37.0 8 35.96438604590501 37.0 35.0 37.0 35.0 37.0 9 37.690652553302925 39.0 37.0 39.0 35.0 39.0 10 37.11194908920775 39.0 37.0 39.0 33.0 39.0 11 36.89534635776259 39.0 37.0 39.0 33.0 39.0 12 36.44676435333647 39.0 35.0 39.0 32.0 39.0 13 36.338814924613025 39.0 35.0 39.0 32.0 39.0 14 37.408760332973806 39.0 35.0 41.0 32.0 41.0 15 37.543504588637404 39.0 35.0 41.0 33.0 41.0 16 37.599802087193424 39.0 35.0 41.0 33.0 41.0 17 37.53967675621738 39.0 35.0 41.0 33.0 41.0 18 37.418031802683416 39.0 36.0 41.0 32.0 41.0 19 37.36255411031362 39.0 35.0 41.0 32.0 41.0 20 37.23407847366994 39.0 35.0 41.0 32.0 41.0 21 37.14463472044816 39.0 35.0 41.0 32.0 41.0 22 37.0333015209889 39.0 35.0 41.0 32.0 41.0 23 36.96291826159684 39.0 35.0 41.0 32.0 41.0 24 36.852048790889384 38.0 35.0 41.0 31.0 41.0 25 36.759139493334935 38.0 35.0 41.0 31.0 41.0 26 36.616179578959134 38.0 35.0 41.0 31.0 41.0 27 36.51627294577479 38.0 35.0 40.0 31.0 41.0 28 36.433480801836694 38.0 35.0 40.0 31.0 41.0 29 36.49304303361412 38.0 35.0 40.0 31.0 41.0 30 36.45235497607864 38.0 35.0 40.0 31.0 41.0 31 36.38512672837295 38.0 35.0 40.0 30.0 41.0 32 36.294646831221364 38.0 35.0 41.0 30.0 41.0 33 36.18152082328415 38.0 35.0 41.0 30.0 41.0 34 36.038665454899274 38.0 35.0 41.0 30.0 41.0 35 35.931155947424756 38.0 35.0 41.0 30.0 41.0 36 35.812663935359524 38.0 35.0 41.0 29.0 41.0 37 35.75211213954923 38.0 35.0 41.0 29.0 41.0 38 35.68544699104217 38.0 35.0 40.0 29.0 41.0 39 35.60099556766403 38.0 35.0 40.0 28.0 41.0 40 35.482025335323506 38.0 35.0 40.0 27.0 41.0 41 35.43908384588472 38.0 34.0 40.0 27.0 41.0 42 35.375688606143164 38.0 34.0 40.0 27.0 41.0 43 35.252918489320784 38.0 34.0 40.0 27.0 41.0 44 35.118463029018244 37.0 34.0 40.0 27.0 41.0 45 35.01079307967647 37.0 34.0 40.0 26.0 41.0 46 34.97696468829768 37.0 34.0 40.0 26.0 41.0 47 34.93624143706228 37.0 34.0 40.0 26.0 41.0 48 34.852895509904954 37.0 34.0 40.0 26.0 41.0 49 34.76827846911546 37.0 34.0 40.0 26.0 41.0 50 34.680943232562036 36.0 34.0 40.0 26.0 41.0 51 34.29622847749984 36.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 2.0 13 3.0 14 5.0 15 31.0 16 66.0 17 147.0 18 378.0 19 730.0 20 1223.0 21 1974.0 22 3083.0 23 4565.0 24 6720.0 25 9877.0 26 13120.0 27 15171.0 28 16697.0 29 18685.0 30 22372.0 31 27583.0 32 34352.0 33 44129.0 34 72735.0 35 112758.0 36 74294.0 37 93909.0 38 137626.0 39 253688.0 40 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.19380342455402 22.719189468388812 28.01139033746618 14.075616769590987 2 32.867189327614014 25.01692402922319 28.349456878401625 13.76642976476117 3 29.254763053239785 25.2195983363731 31.627025449185474 13.898613161201636 4 25.40353717386309 28.421603963224655 30.553100047407984 15.621758815504275 5 22.558851112017404 32.547237191045895 29.902016599005055 14.991895097931646 6 21.013847685807207 41.1063450100509 27.110742152322477 10.769065151819412 7 86.33501089969589 4.41328996917446 7.311180624419045 1.9405185067106103 8 86.78507621506249 3.4603687885707424 7.058717582979498 2.6958374133872693 9 81.02780095271416 5.517544059406966 9.764802573694572 3.6898524141843034 10 42.14538724456102 25.013715191878124 17.50389718471103 15.337000378849828 11 35.0887398792235 25.395256303295167 23.959870901227845 15.556132916253485 12 31.540386840868578 23.32452110690397 27.551802020946464 17.58329003128099 13 22.654391656194818 31.38895042035769 29.0495009740374 16.90715694941009 14 19.45083336611178 33.07048470005652 29.871687910550037 17.606994023281665 15 17.947027270977 25.128922803654348 40.283226475599385 16.640823449769275 16 20.046952536120124 23.004361948571653 37.962305477174816 18.98638003813341 17 20.352516660076475 23.145550791754737 29.97737252117315 26.524560026995637 18 22.86420821420956 24.803484590334982 32.17014704755911 20.162160147896348 19 26.525802157580824 26.537395376375915 26.96499883032703 19.971803635716224 20 28.41384064706723 24.997153450742278 26.62403398469281 19.964971917497685 21 24.245457424939083 27.47975844700553 28.58318445018125 19.691599677874137 22 23.98088361029395 25.12737014042286 27.49725178608027 23.394494463202918 23 21.85487360286187 28.391999850944327 26.73893106382274 23.01419548237106 24 21.394146666639063 25.396601944762452 33.64786840040493 19.56138298819355 25 20.522585039365186 26.454276138050393 31.341024881945838 21.68211394063858 26 19.577737707565195 30.799766479449985 28.712469541922943 20.910026271061877 27 20.136489449135787 29.93224177657797 28.80469773787318 21.126571036413058 28 19.275796464482312 28.150094919478885 32.78624381781257 19.787864798226238 29 19.737144465997712 26.5932912527094 32.50603985997048 21.163524421322414 30 21.024819839309707 28.295941752356423 30.538712034796216 20.14052637353765 31 25.34422543842034 27.03776698044265 27.009094466101217 20.608913115035783 32 25.708583743408948 26.888504288455845 27.95984191818086 19.443070049954354 33 24.963201881413795 27.109085978208892 27.449429758550515 20.4782823818268 34 20.709732714200243 28.40907914649067 29.376388339706157 21.50479979960293 35 20.97844696412934 27.65841822950847 30.295564972745588 21.06756983361661 36 25.146312631846985 27.22481114439561 27.546626476841514 20.082249746915892 37 21.24446993112386 30.363468111402554 28.81225403226641 19.579807925207177 38 21.208448144153394 30.45569630735279 26.993774855550562 21.34208069294325 39 20.904850726956926 29.23923642092493 28.06821781173855 21.787695040379596 40 22.693932813156646 26.92887353247447 27.949387319088853 22.427806335280028 41 19.51107669949342 26.568655662769824 28.4810192095495 25.439248428187256 42 22.344169542544005 26.938500044509677 26.832815433886566 23.884514979059748 43 22.210433482872055 27.095526052653916 28.261472628617447 22.432567835856585 44 21.34321931264634 29.346680716543734 28.095544684612694 21.214555286197236 45 19.923671075540174 31.08856184050629 26.876911069660753 22.110856014292782 46 22.64983717738246 29.356514250343142 27.677464231814692 20.31618434045971 47 21.696294931486147 28.411356385896852 28.174937531182653 21.71741115143435 48 23.034587126144572 25.952144848987974 29.216153494216847 21.797114530650607 49 21.527779215428918 26.231210187126976 29.435389542502605 22.805621054941504 50 20.642864684364266 29.722114685916928 28.71039932428096 20.92462130543784 51 20.01165532532435 30.387068592521132 27.253380147854944 22.347895934299572 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1065.0 1 1257.0 2 1449.0 3 5283.5 4 9118.0 5 6513.5 6 3909.0 7 4083.0 8 4257.0 9 4404.5 10 4552.0 11 4797.0 12 5042.0 13 4916.5 14 4791.0 15 4771.5 16 4752.0 17 4710.5 18 4669.0 19 4458.5 20 4248.0 21 4655.5 22 5063.0 23 5159.5 24 5256.0 25 6015.5 26 7917.0 27 9059.0 28 11159.0 29 13259.0 30 14007.0 31 14755.0 32 18313.0 33 21871.0 34 24392.0 35 26913.0 36 30593.0 37 34273.0 38 39302.5 39 44332.0 40 51066.5 41 57801.0 42 67729.5 43 77658.0 44 82453.0 45 87248.0 46 87758.5 47 88269.0 48 83520.0 49 78771.0 50 73532.5 51 68294.0 52 61534.0 53 54774.0 54 50210.5 55 45647.0 56 41507.5 57 37368.0 58 34673.0 59 31978.0 60 29790.5 61 27603.0 62 25250.5 63 22898.0 64 20399.0 65 17900.0 66 15346.0 67 12792.0 68 10925.5 69 9059.0 70 8013.0 71 6967.0 72 5685.0 73 4403.0 74 3864.5 75 2693.0 76 2060.0 77 1510.5 78 961.0 79 737.5 80 514.0 81 369.5 82 225.0 83 152.0 84 79.0 85 63.5 86 48.0 87 32.5 88 17.0 89 13.5 90 10.0 91 5.0 92 0.0 93 1.5 94 3.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 966082.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.319434159470152 #Duplication Level Percentage of deduplicated Percentage of total 1 76.2397207257468 20.82826030703432 2 6.696856979096652 3.6590868663163834 3 2.8197893455625427 2.3110514810901397 4 1.8087809207411079 1.9765948509237 5 1.2400358336283694 1.6938538656096962 6 0.9679218151357292 1.5865845780069239 7 0.7605036472364624 1.4543570523099416 8 0.6860757685284334 1.4994561429376336 9 0.5582789023926451 1.3726677344883418 >10 7.316440285598448 46.43193394810894 >50 0.8765678230893623 15.159029419241774 >100 0.02788211298386432 1.1875583179853972 >500 3.8194675320361914E-4 0.0786766515913353 >1k 3.8194675320361914E-4 0.20023938249837192 >5k 3.8194675320361914E-4 0.5606494018570882 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5373 0.5561639695181154 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1919 0.19863738274804832 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.05413619133779534 0.0 2 0.0 0.0 0.0 0.1764860539788548 0.0 3 0.0 0.0 0.0 0.28082502313468216 0.0 4 0.0 0.0 0.0 0.4524460656548823 0.0 5 0.0 0.0 0.0 0.7668086145896518 0.0 6 0.0 0.0 0.0 1.4463575555698172 0.0 7 0.0 0.0 0.0 1.7816293026885917 0.0 8 0.0 0.0 0.0 2.67968971577982 0.0 9 0.0 0.0 0.0 3.160911806658234 0.0 10 0.0 0.0 0.0 3.831041257367387 0.0 11 0.0 0.0 0.0 4.271997615109276 0.0 12 0.0 0.0 0.0 4.678795381758484 0.0 13 0.0 0.0 0.0 4.893269929467685 0.0 14 0.0 0.0 0.0 4.995021126571037 0.0 15 0.0 0.0 0.0 5.0942880625040114 0.0 16 0.0 0.0 0.0 5.258663343277279 0.0 17 0.0 0.0 0.0 5.429973853151182 0.0 18 0.0 0.0 0.0 5.702000451307446 0.0 19 0.0 0.0 0.0 5.831803097459636 0.0 20 0.0 0.0 0.0 5.967195331245174 0.0 21 0.0 0.0 0.0 6.13488296024561 0.0 22 0.0 0.0 0.0 6.3141638080411395 0.0 23 0.0 0.0 0.0 6.519736419889823 0.0 24 0.0 0.0 0.0 6.676037851859366 0.0 25 0.0 0.0 0.0 6.818365314745539 0.0 26 0.0 0.0 0.0 6.953550526766879 0.0 27 0.0 0.0 0.0 7.092669152307982 0.0 28 0.0 0.0 0.0 7.23334044108057 0.0 29 0.0 0.0 0.0 7.387054100997638 0.0 30 0.0 0.0 0.0 7.606911214575988 0.0 31 0.0 0.0 0.0 7.772735647698642 0.0 32 0.0 0.0 0.0 7.934523156419434 0.0 33 0.0 0.0 0.0 8.096310665140226 0.0 34 0.0 0.0 0.0 8.262135098262881 0.0 35 0.0 0.0 0.0 8.441726478704707 0.0 36 0.0 0.0 0.0 8.60651580300637 0.0 37 0.0 0.0 0.0 8.777515780233976 0.0 38 0.0 0.0 0.0 8.95627907361901 0.0 39 0.0 0.0 0.0 9.157400717537435 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTACG 30 2.1651413E-6 45.000004 1 CTTAACG 20 7.032729E-4 45.0 1 GTCGCAA 20 7.032729E-4 45.0 9 ACGCATG 20 7.032729E-4 45.0 1 CGATCGA 20 7.032729E-4 45.0 10 AACGGGC 110 0.0 40.909092 4 TACGGGT 160 0.0 40.78125 4 CGTTTTT 3420 0.0 40.131577 1 GTACGAG 80 0.0 39.375 1 GACCGAT 800 0.0 39.09375 9 ACGGGTA 110 0.0 38.863636 5 TCACGAC 35 6.247252E-6 38.57143 25 TGATTCG 70 0.0 38.57143 15 CGGGATC 140 0.0 38.57143 6 ACGTAAG 35 6.247252E-6 38.57143 1 AGGGCGA 1185 0.0 38.544304 6 TAGGGCG 535 0.0 38.271027 5 TTGGGCG 410 0.0 37.865852 5 TACGCGG 30 1.1397869E-4 37.500004 2 AGTTGCG 30 1.1397869E-4 37.500004 1 >>END_MODULE