FastQCFastQC Report
Thu 26 May 2016
SRR1546672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546672_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1576008
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197961.2560849944924137No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29220.18540515022766382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16220.10291825929817616No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT207.034083E-445.0000049
TGCGCGA207.034083E-445.00000415
GTCGTAA207.034083E-445.00000428
CGTCGGT207.034083E-445.00000425
CGCGATT253.8914673E-545.026
ACTACGG453.8562575E-1045.02
TATTACG253.8914673E-545.01
TCGCGAT253.8914673E-545.025
CTATACG302.166018E-644.9999961
CGGGTAC1300.043.2692346
CGTTTTT115050.043.2203371
GCTACGA1400.041.78571310
CGCCAGT650.041.53846426
CGCATCG556.184564E-1140.90909221
TAATGCG556.184564E-1140.9090921
CGTAAGG2150.040.8139532
ACTCCGT501.0822987E-940.517
CGAATAT1450.040.3448314
CGGTCTA950.040.26315731
CGTTAGG1850.040.1351362