##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546672_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1576008
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32446789610205	31.0	31.0	33.0	30.0	34.0
2	31.715846620067918	31.0	31.0	34.0	30.0	34.0
3	31.72002616737986	31.0	31.0	34.0	30.0	34.0
4	35.541404612159326	37.0	35.0	37.0	33.0	37.0
5	35.47088656910371	37.0	35.0	37.0	33.0	37.0
6	35.55224910025837	37.0	35.0	37.0	33.0	37.0
7	35.991617428337925	37.0	35.0	37.0	35.0	37.0
8	35.983231684103124	37.0	35.0	37.0	35.0	37.0
9	37.70670834158202	39.0	37.0	39.0	35.0	39.0
10	37.125318526301896	39.0	37.0	39.0	33.0	39.0
11	36.86163902721306	39.0	37.0	39.0	32.0	39.0
12	36.346912579123966	38.0	35.0	39.0	32.0	39.0
13	36.16123712569987	38.0	35.0	39.0	32.0	39.0
14	37.22558768737215	39.0	35.0	41.0	32.0	41.0
15	37.42827130319135	39.0	35.0	41.0	32.0	41.0
16	37.491793188867064	39.0	35.0	41.0	33.0	41.0
17	37.415754869264624	39.0	35.0	41.0	32.0	41.0
18	37.26708430414059	39.0	35.0	41.0	32.0	41.0
19	37.16248521581109	38.0	35.0	41.0	32.0	41.0
20	36.94450662686992	38.0	35.0	41.0	32.0	41.0
21	36.847421459789544	38.0	35.0	41.0	32.0	41.0
22	36.73660920502942	38.0	35.0	41.0	31.0	41.0
23	36.662950949487566	38.0	35.0	40.0	31.0	41.0
24	36.53621555220532	38.0	35.0	40.0	31.0	41.0
25	36.441502200496444	38.0	35.0	40.0	31.0	41.0
26	36.28207280673702	38.0	35.0	40.0	31.0	41.0
27	36.193342927193264	38.0	35.0	40.0	31.0	41.0
28	36.11243851554053	37.0	35.0	40.0	30.0	41.0
29	36.10631671920447	38.0	35.0	40.0	30.0	41.0
30	36.05640390150304	37.0	35.0	40.0	30.0	41.0
31	35.91137735341445	37.0	35.0	40.0	30.0	41.0
32	35.670443931756694	38.0	35.0	40.0	30.0	41.0
33	35.42794833528764	38.0	34.0	40.0	28.0	41.0
34	35.203723585159466	38.0	34.0	40.0	27.0	41.0
35	35.005663676834125	37.0	34.0	40.0	25.0	41.0
36	34.836217201943136	37.0	34.0	40.0	24.0	41.0
37	34.76257227120674	37.0	34.0	40.0	24.0	41.0
38	34.689641169334166	37.0	34.0	40.0	24.0	41.0
39	34.61316693823889	37.0	34.0	40.0	23.0	41.0
40	34.492722752676386	37.0	34.0	40.0	23.0	41.0
41	34.45214174039726	37.0	34.0	40.0	23.0	41.0
42	34.375982228516605	37.0	34.0	40.0	23.0	41.0
43	34.25696189359445	36.0	33.0	40.0	23.0	41.0
44	34.1210101725372	36.0	33.0	40.0	22.0	41.0
45	34.008855919513096	36.0	33.0	40.0	23.0	41.0
46	33.9780952888564	36.0	33.0	40.0	22.0	41.0
47	33.951246440373396	36.0	33.0	40.0	22.0	41.0
48	33.858670768168686	36.0	33.0	40.0	22.0	41.0
49	33.80183412774554	36.0	33.0	40.0	21.0	41.0
50	33.68504855305303	35.0	33.0	40.0	21.0	41.0
51	33.334144242922626	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	1.0
13	4.0
14	8.0
15	33.0
16	108.0
17	299.0
18	747.0
19	1423.0
20	2611.0
21	4134.0
22	6445.0
23	9980.0
24	15258.0
25	23298.0
26	31230.0
27	33624.0
28	32939.0
29	34622.0
30	39596.0
31	47083.0
32	58703.0
33	75232.0
34	121936.0
35	203796.0
36	125147.0
37	146209.0
38	201637.0
39	359630.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.266211846640374	24.187821381617354	27.664072771204207	16.881894000538068
2	33.55363678356963	25.62252222069939	27.80975731087659	13.014083684854391
3	26.51782224455713	24.98502545672357	35.096712707042094	13.400439591677198
4	23.9785584844747	27.47860416952198	34.47216003979675	14.070677306206566
5	21.214930381064057	31.74844290130507	33.275211800955326	13.761414916675548
6	19.45358145390125	40.128349602286285	29.97281739686601	10.445251546946462
7	84.46727427779554	3.8077852396688345	10.134593225415099	1.5903472571205224
8	84.88478484880787	3.0048070821975523	9.752805823320694	2.35760224567388
9	79.1850675884894	5.6977502652270795	12.26154943375922	2.855632712524302
10	40.573588458941835	31.39812742067299	16.68525794285308	11.343026177532094
11	34.0078222953183	22.76492251308369	28.714130892736584	14.513124298861428
12	31.510055786518848	22.53421302429937	30.061585981797045	15.894145207384733
13	20.564552971812326	33.71372480342739	31.319193811198925	14.402528413561352
14	16.4556271287963	36.70108273562063	31.65535961746387	15.187930518119197
15	15.478538180009238	25.277727016614126	44.54006578646809	14.703669016908544
16	17.554352515977076	22.79258734727235	42.64940279490967	17.003657341840906
17	17.70314617692296	22.894173126024743	32.20053451505323	27.202146181999076
18	20.657192095471597	25.13673788457927	35.51124105969005	18.694828960259084
19	25.093400541114004	26.950497713209575	29.275676265602712	18.680425480073705
20	26.599991878213817	26.188572646839358	28.374602159379904	18.836833315566924
21	22.235293221861816	29.35860731671413	29.997119303962922	18.40898015746113
22	21.686247785544236	25.623537443972367	28.962670240252585	23.72754453023081
23	18.24381602123847	30.853396683265565	28.407660367206255	22.495126928289704
24	19.22185674184395	26.933365820478066	36.50971314866422	17.33506428901376
25	17.986901081720397	28.33868863609829	34.110550200252796	19.563860081928517
26	17.042426180577763	33.457000218273	30.002005065964134	19.4985685351851
27	18.3717976050883	31.83391201059893	30.59407058847417	19.2002197958386
28	16.46945954589063	29.823706478647317	36.43357140319085	17.273262572271207
29	16.97015497383262	26.614839518581125	35.69632895264491	20.718676554941347
30	19.20840503347699	29.694328962797144	32.50782990949285	18.589436094233026
31	24.850698727417626	28.6285348805336	27.29231069893046	19.22845569311831
32	24.862944858147927	29.00340607408084	28.597253313434955	17.536395754336272
33	23.409272034152114	29.160194618301432	27.300940096750775	20.129593250795683
34	18.917860823041508	30.35955401241618	29.27954680433094	21.443038360211368
35	18.88321632885112	28.806325856213927	30.973446835295253	21.337010979639697
36	25.481342734300842	28.548332241968318	27.32987396003066	18.640451063700183
37	19.566461591565528	31.865828092243188	29.024535408449704	19.543174907741584
38	19.832703894904085	33.68199907614682	25.901201009131935	20.584096019817157
39	19.594634037390673	32.05510378119908	27.224417642550037	21.125844538860207
40	22.3163207293364	29.36311236998797	26.950814970482384	21.36975193019325
41	17.76977020421216	27.914642565266167	28.063182420393808	26.252404810127867
42	21.03656834229268	29.06971284409724	26.01903036025198	23.874688453358107
43	21.274892005624338	28.356074334648046	28.153981451870802	22.215052207856814
44	20.31855168247877	31.340703854295153	28.25448855589566	20.08625590733042
45	18.248574880330555	34.35782051867757	26.111034969365637	21.282569631626234
46	22.053822061816945	32.12286993467038	26.62708564931143	19.196222354201247
47	20.91023649626144	29.515015152207347	27.414264394596984	22.16048395693423
48	21.832693742671356	28.073778813305516	29.133037395749263	20.960490048273865
49	20.567027578540213	27.195293424906474	29.44915254237288	22.788526454180435
50	19.15370987964528	31.83169120968929	28.64122517144583	20.3733737392196
51	18.733153638814017	32.88244729722184	26.113509576093524	22.270889487870623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2022.0
1	1944.5
2	1867.0
3	14565.0
4	27263.0
5	18556.0
6	9849.0
7	10675.5
8	11502.0
9	13207.0
10	14912.0
11	16240.0
12	17568.0
13	18326.0
14	19084.0
15	18643.0
16	18202.0
17	16972.0
18	15742.0
19	14756.0
20	13770.0
21	13693.5
22	13617.0
23	12601.5
24	11586.0
25	12057.0
26	13556.0
27	14584.0
28	16414.5
29	18245.0
30	21318.5
31	24392.0
32	27380.5
33	30369.0
34	35618.5
35	40868.0
36	44702.0
37	48536.0
38	57837.0
39	67138.0
40	81846.5
41	96555.0
42	112588.5
43	128622.0
44	133549.5
45	138477.0
46	143903.0
47	149329.0
48	147703.0
49	146077.0
50	133541.5
51	121006.0
52	105219.5
53	89433.0
54	80355.5
55	71278.0
56	62301.5
57	53325.0
58	47260.5
59	41196.0
60	37154.0
61	33112.0
62	27877.5
63	22643.0
64	20440.5
65	18238.0
66	14896.5
67	11555.0
68	9424.5
69	7294.0
70	6079.0
71	4864.0
72	4339.5
73	3815.0
74	3095.5
75	1988.0
76	1600.0
77	1179.5
78	759.0
79	603.0
80	447.0
81	323.0
82	199.0
83	138.0
84	77.0
85	69.0
86	61.0
87	35.0
88	9.0
89	8.0
90	7.0
91	7.5
92	8.0
93	4.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1576008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.432361596311072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10778765014085	21.923565003603738
2	7.5361003891103255	4.285382625785707
3	3.2161082525845446	2.743246583110917
4	1.9515476937862593	2.2194843880871153
5	1.251432491307046	1.7790590553107173
6	0.9354286353144932	1.5957867124083283
7	0.7554280720887416	1.5035022873941872
8	0.5658929784442618	1.2871739030352574
9	0.5091120203470978	1.302773134998345
>10	4.9524785364379875	31.098588040554603
>50	0.9908963586463889	19.584798989223714
>100	0.2246294795921493	8.547403318719555
>500	0.0020297842714068395	0.4245005884783264
>1k	9.021263428474842E-4	0.43533753703615674
>5k	0.0	0.0
>10k+	2.2553158571187105E-4	1.2693978322533155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19796	1.2560849944924137	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2922	0.18540515022766382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1622	0.10291825929817616	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06992350292638108	0.0
2	0.0	0.0	0.0	0.21966893569068177	0.0
3	0.0	0.0	0.0	0.3657976355449972	0.0
4	0.0	0.0	0.0	0.5664945863218969	0.0
5	0.0	0.0	0.0	0.9689671626032356	0.0
6	0.0	0.0	0.0	1.8250541875421953	0.0
7	0.0	0.0	0.0	2.3116633925716115	0.0
8	0.0	0.0	0.0	3.4758072294049267	0.0
9	0.0	0.0	0.0	4.115397891381262	0.0
10	0.0	0.0	0.0	4.95308399449749	0.0
11	0.0	0.0	0.0	5.473449373353435	0.0
12	0.0	0.0	0.0	5.965959563657037	0.0
13	0.0	0.0	0.0	6.247937827726763	0.0
14	0.0	0.0	0.0	6.391338115034949	0.0
15	0.0	0.0	0.0	6.511324815610073	0.0
16	0.0	0.0	0.0	6.709229902386283	0.0
17	0.0	0.0	0.0	6.907896406617225	0.0
18	0.0	0.0	0.0	7.246283013791808	0.0
19	0.0	0.0	0.0	7.3988203105568	0.0
20	0.0	0.0	0.0	7.576801640600809	0.0
21	0.0	0.0	0.0	7.77089964010335	0.0
22	0.0	0.0	0.0	7.973182877244278	0.0
23	0.0	0.0	0.0	8.20401926893772	0.0
24	0.0	0.0	0.0	8.387013263892062	0.0
25	0.0	0.0	0.0	8.540946492657397	0.0
26	0.0	0.0	0.0	8.691199537058187	0.0
27	0.0	0.0	0.0	8.853635260734718	0.0
28	0.0	0.0	0.0	9.023685158958584	0.0
29	0.0	0.0	0.0	9.20813853736783	0.0
30	0.0	0.0	0.0	9.49531982071157	0.0
31	0.0	0.0	0.0	9.699760407307577	0.0
32	0.0	0.0	0.0	9.894746727173974	0.0
33	6.345145456114435E-5	0.0	0.0	10.084910736493724	0.0
34	6.345145456114435E-5	0.0	0.0	10.289478225998852	0.0
35	6.345145456114435E-5	0.0	0.0	10.503119273506226	0.0
36	6.345145456114435E-5	0.0	0.0	10.70736950573855	0.0
37	6.345145456114435E-5	0.0	0.0	10.906416718696859	0.0
38	6.345145456114435E-5	0.0	0.0	11.118154222567398	0.0
39	6.345145456114435E-5	0.0	0.0	11.336554129166856	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	20	7.034083E-4	45.000004	9
TGCGCGA	20	7.034083E-4	45.000004	15
GTCGTAA	20	7.034083E-4	45.000004	28
CGTCGGT	20	7.034083E-4	45.000004	25
CGCGATT	25	3.8914673E-5	45.0	26
ACTACGG	45	3.8562575E-10	45.0	2
TATTACG	25	3.8914673E-5	45.0	1
TCGCGAT	25	3.8914673E-5	45.0	25
CTATACG	30	2.166018E-6	44.999996	1
CGGGTAC	130	0.0	43.269234	6
CGTTTTT	11505	0.0	43.220337	1
GCTACGA	140	0.0	41.785713	10
CGCCAGT	65	0.0	41.538464	26
CGCATCG	55	6.184564E-11	40.909092	21
TAATGCG	55	6.184564E-11	40.909092	1
CGTAAGG	215	0.0	40.813953	2
ACTCCGT	50	1.0822987E-9	40.5	17
CGAATAT	145	0.0	40.34483	14
CGGTCTA	95	0.0	40.263157	31
CGTTAGG	185	0.0	40.135136	2
>>END_MODULE