FastQCFastQC Report
Thu 26 May 2016
SRR1546671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546671_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1702764
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206611.2133801278392073No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39550.23226941607879895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17860.104888287513713No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA207.034245E-445.044
GTTACGC253.8916005E-545.011
CGTAGAT207.034245E-445.023
CTACGAT253.8916005E-545.020
CGAATCT207.034245E-445.025
CGTTTTT139200.043.8523671
TTGTGCG1300.041.538461
CGACGGT556.184564E-1140.90909228
TACGGGA4000.040.54
CGTTGAT1400.040.1785725
GCGTAAG900.040.01
CTATGCG800.039.3751
GCGCGTT403.459536E-739.37513
GCCGACC403.459536E-739.37514
ATACCGG403.459536E-739.3752
AATCGTT1500.039.00000422
TAGTACG750.039.0000041
CTACGGG2850.038.6842083
AGGGCGA18450.038.658546
AGGTACG356.250062E-638.5714261
Produced by FastQC (version 0.11.5)