FastQCFastQC Report
Thu 26 May 2016
SRR1546670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546670_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2925344
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT484371.655771081965061No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT315100.043.172011
TAGCCGT1400.040.17857444
CGAATAT2600.038.07692714
GTCGAAC301.1403535E-437.49999630
GTTTTTT373750.037.2762572
AGGGCGA35400.036.9915246
TAGGGAT39150.036.896555
GACCGAT19250.036.8181849
GGGCGAT69550.036.653497
AGGGATT41400.036.6304366
ACACGAC11250.036.626
ATGGGCG10350.036.521745
GGCGATT18550.036.5094348
CACGACC11400.036.3157927
TAGGGAC24750.036.2727285
ACGGGAT10700.036.168235
AAGGGAT38200.036.106025
GCGAGAC11600.036.07758321
ATACTAT21900.036.06164645
CGTACGT250.002107639836.035
Produced by FastQC (version 0.11.5)