##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546670_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2925344
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.436321677040375	31.0	31.0	33.0	30.0	34.0
2	31.845777795705395	31.0	31.0	34.0	30.0	34.0
3	31.78682028506733	31.0	31.0	34.0	30.0	34.0
4	35.65998357799972	37.0	35.0	37.0	33.0	37.0
5	35.62786154380476	37.0	35.0	37.0	33.0	37.0
6	35.700706993775775	37.0	35.0	37.0	33.0	37.0
7	36.03927196254526	37.0	35.0	37.0	35.0	37.0
8	36.018672333920385	37.0	35.0	37.0	35.0	37.0
9	37.7573140116171	39.0	38.0	39.0	35.0	39.0
10	37.23404461150552	39.0	37.0	39.0	34.0	39.0
11	37.047065575877575	39.0	37.0	39.0	33.0	39.0
12	36.805124798998	39.0	35.0	39.0	33.0	39.0
13	36.76860977717492	39.0	35.0	39.0	33.0	39.0
14	37.91498469923537	40.0	37.0	41.0	33.0	41.0
15	38.00935889932945	40.0	37.0	41.0	33.0	41.0
16	38.00710514729208	40.0	36.0	41.0	33.0	41.0
17	37.915628042377236	40.0	36.0	41.0	33.0	41.0
18	37.68539323922246	39.0	36.0	41.0	33.0	41.0
19	37.524692822450966	39.0	36.0	41.0	33.0	41.0
20	37.28779931522583	39.0	35.0	41.0	32.0	41.0
21	37.20406591498299	39.0	35.0	41.0	32.0	41.0
22	37.11622120338668	39.0	35.0	41.0	32.0	41.0
23	37.003289869499106	39.0	35.0	41.0	32.0	41.0
24	36.87182225406653	38.0	35.0	41.0	32.0	41.0
25	36.772348140936586	38.0	35.0	41.0	31.0	41.0
26	36.66223767187722	38.0	35.0	41.0	31.0	41.0
27	36.56612658203616	38.0	35.0	40.0	31.0	41.0
28	36.44817361650459	38.0	35.0	40.0	31.0	41.0
29	36.4315919768752	38.0	35.0	40.0	31.0	41.0
30	36.34879214205235	38.0	35.0	40.0	31.0	41.0
31	36.201500746578866	38.0	35.0	40.0	30.0	41.0
32	35.92941035310719	38.0	35.0	41.0	29.0	41.0
33	35.55616809510266	38.0	35.0	41.0	26.0	41.0
34	35.15957234431233	38.0	34.0	41.0	24.0	41.0
35	34.86768256998151	38.0	34.0	41.0	22.0	41.0
36	34.68367720172397	38.0	34.0	41.0	21.0	41.0
37	34.6140529113841	38.0	34.0	40.0	21.0	41.0
38	34.503214322828356	38.0	34.0	40.0	21.0	41.0
39	34.39858457671986	38.0	34.0	40.0	20.0	41.0
40	34.27112742979971	38.0	34.0	40.0	18.0	41.0
41	34.174116616712425	38.0	34.0	40.0	18.0	41.0
42	34.08354812288743	38.0	33.0	40.0	18.0	41.0
43	33.97357917564567	38.0	33.0	40.0	18.0	41.0
44	33.81889719636391	37.0	33.0	40.0	18.0	41.0
45	33.717383323123705	37.0	33.0	40.0	18.0	41.0
46	33.674905925593706	37.0	33.0	40.0	18.0	41.0
47	33.64626279849481	37.0	33.0	40.0	18.0	41.0
48	33.551195688438696	37.0	33.0	40.0	18.0	41.0
49	33.463586162858114	36.0	33.0	40.0	18.0	41.0
50	33.394514286183096	36.0	33.0	40.0	18.0	41.0
51	33.05106544734568	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	8.0
12	6.0
13	20.0
14	29.0
15	81.0
16	247.0
17	541.0
18	1349.0
19	2831.0
20	4713.0
21	7667.0
22	11953.0
23	18265.0
24	28558.0
25	47519.0
26	69484.0
27	78088.0
28	73176.0
29	68331.0
30	71687.0
31	80898.0
32	97212.0
33	121698.0
34	185710.0
35	264820.0
36	228048.0
37	282601.0
38	418283.0
39	761017.0
40	500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.540940142424272	21.4778159423302	25.915823916776965	21.065419998468556
2	38.57187394029557	23.297157530875	25.382450747672753	12.748517781156677
3	27.094728004638085	24.056828872091625	35.764067405405996	13.08437571786429
4	23.865159106074362	26.398194537121107	35.45603525602459	14.280611100779941
5	21.574556701707557	29.728572092717986	34.53778427425971	14.159086931314743
6	20.02379891048711	37.77148943850706	32.05517026373651	10.149541387269325
7	81.00596032466609	3.4350148221884336	13.886332684292856	1.6726921688526204
8	81.07942177056783	3.0828852948576304	13.697260903333078	2.140432031241454
9	75.15064894932016	5.4502308104619495	16.03838728026516	3.360732959952744
10	34.24534003522321	30.182843453624596	22.78576468271766	12.786051828434536
11	27.072064003412933	24.49250412942888	32.813064036229584	15.622367830928601
12	25.217000120327732	22.253348666003042	35.24877757966243	17.280873634006806
13	21.79552216764934	25.396808033516745	36.1044718159642	16.703197982869707
14	18.90266580614109	27.83652110657755	36.05189680256407	17.208916284717287
15	18.882292133848193	24.058196232648193	40.33559813820187	16.723913495301748
16	21.37697310128313	22.554714932671168	38.45824627804457	17.610065688001136
17	21.167698568100025	22.57857537438332	34.94775315313344	21.30597290438321
18	22.11934733145914	22.690664756008182	36.67602852861066	18.513959383922028
19	24.1907618386077	25.11407205443189	32.47163410525394	18.223532001706467
20	25.34433557215835	24.232842359736154	32.231115383353206	18.19170668475229
21	23.776725062078167	24.965713434043995	33.79192327466445	17.465638229213383
22	22.737394303028978	22.91798844853802	33.628557872168194	20.71605937626481
23	20.347316418171673	25.639548716321908	33.45490308148375	20.558231784022666
24	20.26288190380345	24.687011168601025	36.69093275867727	18.359174168918255
25	20.76203687497949	25.82858631326777	34.23987059299692	19.16950621875581
26	19.401137097038845	26.969648697725805	33.434768697288256	20.1944455079471
27	19.167523545948782	25.823151055055405	34.36652236454926	20.642803034446548
28	19.123152695888074	25.88567361650459	35.85752649944759	19.133647188159753
29	19.66466166030388	24.454354769900565	35.51736137698677	20.363622192808776
30	20.708504709189757	25.301366266668126	35.38021511316276	18.60991391097936
31	22.347012864128114	25.953494700110486	32.74599500092981	18.953497434831597
32	22.466793648883687	25.573060809258674	32.3408802520319	19.619265289825744
33	21.962271787523107	26.449778214117725	31.437772788431033	20.15017720992813
34	19.94599609481825	27.43027828522047	32.2687519826728	20.35497363728847
35	20.26571917695833	27.69619572945951	31.77424603738911	20.26383905619305
36	22.167683527133903	28.75084092674229	29.4920187164313	19.58945682969251
37	21.040260564227662	29.34359172801558	29.848455429515298	19.767692278241462
38	21.066513886913814	29.14908468884343	30.077283218657364	19.707118205585395
39	21.136317643326734	28.974130905630247	28.783931052211297	21.105620398831725
40	22.363626294890445	27.3545948784143	30.228957688394935	20.052821138300317
41	20.083005622586608	27.54896518153079	30.234871522802102	22.1331576730805
42	21.618619895643043	28.097310948729447	29.259362317730837	21.02470683789667
43	21.39468042049072	27.204151033177638	30.076120962184277	21.32504758414737
44	21.143462102234814	28.125581128236544	29.82028780204995	20.910668967478696
45	20.32721621799009	28.95396233742083	29.013681809729043	21.70513963486004
46	21.504718761280724	28.31530240546069	29.479644103394335	20.70033472986425
47	20.80240819541223	28.193880788037234	30.536340341511973	20.46737067503856
48	21.351095802750038	27.153080116389734	30.60542623363269	20.89039784722754
49	21.217641412428762	26.842962742159553	30.591308235886107	21.34808760952558
50	20.36724569828369	28.264060568603217	30.700355240272597	20.668338492840498
51	20.14334040714528	27.944064014351817	30.426575472833278	21.486020105669624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2835.0
1	3682.0
2	4529.0
3	42147.5
4	79766.0
5	55513.0
6	31260.0
7	31287.0
8	31314.0
9	32465.5
10	33617.0
11	33925.5
12	34234.0
13	33667.5
14	33101.0
15	31688.5
16	30276.0
17	28864.5
18	27453.0
19	26082.5
20	24712.0
21	24113.0
22	23514.0
23	24709.0
24	25904.0
25	26591.0
26	30745.5
27	34213.0
28	37758.0
29	41303.0
30	46506.0
31	51709.0
32	59651.5
33	67594.0
34	78113.0
35	88632.0
36	94563.5
37	100495.0
38	110130.0
39	119765.0
40	132120.5
41	144476.0
42	163108.0
43	181740.0
44	193156.5
45	204573.0
46	210277.0
47	215981.0
48	208388.0
49	200795.0
50	191338.5
51	181882.0
52	174494.0
53	167106.0
54	154974.5
55	142843.0
56	132519.0
57	122195.0
58	114275.0
59	106355.0
60	98488.5
61	90622.0
62	80970.0
63	71318.0
64	64236.0
65	57154.0
66	49483.0
67	41812.0
68	35067.5
69	28323.0
70	23749.0
71	19175.0
72	16821.0
73	14467.0
74	11981.5
75	7523.0
76	5550.0
77	4449.0
78	3348.0
79	2422.5
80	1497.0
81	1074.5
82	652.0
83	461.0
84	270.0
85	193.0
86	116.0
87	80.5
88	45.0
89	37.0
90	29.0
91	21.5
92	14.0
93	8.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2925344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.18525018462058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.94535631197158	20.1483340557375
2	6.543915064494836	3.427081063014096
3	2.819706810078418	2.215041848075454
4	1.7475924448486706	1.830445815564607
5	1.20972319403764	1.5838452245006955
6	0.9348044920125642	1.4686853698233686
7	0.770895014285716	1.4130255170603692
8	0.6623378822879621	1.3874786523569649
9	0.5249708682863555	1.2371844173144142
>10	6.653219001148224	40.67780884209685
>50	1.0930359345349578	18.825185479196254
>100	0.09099726037120905	3.3027996812528624
>500	0.00238549959821439	0.4049113874860415
>1k	9.276942881944789E-4	0.3972767881333715
>5k	0.0	0.0
>10k+	1.3252775545635414E-4	1.6808958583871516
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48437	1.655771081965061	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025603826421781505	0.0
2	0.0	0.0	0.0	0.08692994738396578	0.0
3	0.0	0.0	0.0	0.17833800059069976	0.0
4	0.0	0.0	0.0	0.2940167036765591	0.0
5	0.0	0.0	0.0	0.5094101753503177	0.0
6	0.0	0.0	0.0	1.0498594353347845	0.0
7	0.0	0.0	0.0	1.3598058894953893	0.0
8	0.0	0.0	0.0	1.993372403382303	0.0
9	0.0	0.0	0.0	2.396333559403612	0.0
10	0.0	0.0	0.0	2.9027013575155607	0.0
11	0.0	0.0	0.0	3.191487907063238	0.0
12	0.0	0.0	0.0	3.4458853386131683	0.0
13	0.0	0.0	0.0	3.6050461073979676	0.0
14	0.0	0.0	0.0	3.697069472855158	0.0
15	0.0	0.0	0.0	3.7675910935602785	0.0
16	0.0	0.0	0.0	3.862417548158439	0.0
17	0.0	0.0	0.0	3.9563894024087425	0.0
18	0.0	0.0	0.0	4.106423039478434	0.0
19	0.0	0.0	0.0	4.187268232385661	0.0
20	0.0	0.0	0.0	4.28007783016288	0.0
21	0.0	0.0	0.0	4.375690517081068	0.0
22	0.0	0.0	0.0	4.4823446404935625	0.0
23	0.0	0.0	0.0	4.605407090584902	0.0
24	0.0	3.418401391426102E-5	0.0	4.713496942581796	0.0
25	0.0	3.418401391426102E-5	0.0	4.804528971635473	0.0
26	0.0	3.418401391426102E-5	0.0	4.8948431363969505	0.0
27	0.0	3.418401391426102E-5	0.0	5.002044204032073	0.0
28	0.0	3.418401391426102E-5	0.0	5.0976910749641755	0.0
29	0.0	3.418401391426102E-5	0.0	5.21654889134406	0.0
30	0.0	3.418401391426102E-5	0.0	5.3767009965323735	0.0
31	0.0	3.418401391426102E-5	0.0	5.495592996926174	0.0
32	0.0	3.418401391426102E-5	0.0	5.616296750057429	0.0
33	0.0	3.418401391426102E-5	0.0	5.736043350799085	0.0
34	0.0	3.418401391426102E-5	0.0	5.862968594462736	0.0
35	0.0	3.418401391426102E-5	0.0	5.996320432742269	0.0
36	0.0	3.418401391426102E-5	0.0	6.117571130096152	0.0
37	0.0	3.418401391426102E-5	0.0	6.2514699125983135	0.0
38	0.0	3.418401391426102E-5	0.0	6.403691326558517	0.0
39	0.0	3.418401391426102E-5	0.0	6.624041480249844	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	31510	0.0	43.17201	1
TAGCCGT	140	0.0	40.178574	44
CGAATAT	260	0.0	38.076927	14
GTCGAAC	30	1.1403535E-4	37.499996	30
GTTTTTT	37375	0.0	37.276257	2
AGGGCGA	3540	0.0	36.991524	6
TAGGGAT	3915	0.0	36.89655	5
GACCGAT	1925	0.0	36.818184	9
GGGCGAT	6955	0.0	36.65349	7
AGGGATT	4140	0.0	36.630436	6
ACACGAC	1125	0.0	36.6	26
ATGGGCG	1035	0.0	36.52174	5
GGCGATT	1855	0.0	36.509434	8
CACGACC	1140	0.0	36.31579	27
TAGGGAC	2475	0.0	36.272728	5
ACGGGAT	1070	0.0	36.16823	5
AAGGGAT	3820	0.0	36.10602	5
GCGAGAC	1160	0.0	36.077583	21
ATACTAT	2190	0.0	36.061646	45
CGTACGT	25	0.0021076398	36.0	35
>>END_MODULE