FastQCFastQC Report
Thu 26 May 2016
SRR1546669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546669_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2431841
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117470.48304967306661906No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48630.19997195540333434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC406.8212103E-945.00000435
TCTAGCG502.1827873E-1145.0000041
TCGTATA207.0348405E-445.00000428
TATCGTT253.8920938E-545.00000419
AGTACGA551.8189894E-1245.034
GTACGAC501.0822987E-940.50000435
AACACGT12450.040.30120541
TACGGGA5650.040.221244
CACGACC13650.040.2197827
ACACGAC13650.040.05494726
CGTTTTT84400.039.961491
CACGTGA12650.039.48616843
ACGACCA13950.039.3548428
AGGGTAC12850.039.221796
TAGGGCG12800.039.023445
CGAGACA14000.038.89285722
GACACGA14150.038.79858825
GCGAGAC14000.038.73214321
AGGGCGA31250.038.6646
AGACACG14650.038.2423224