##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546669_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2431841
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.346353236087392	31.0	31.0	33.0	30.0	34.0
2	31.74712368119462	31.0	31.0	34.0	30.0	34.0
3	31.867100686270195	31.0	31.0	34.0	30.0	34.0
4	35.62829930081777	37.0	35.0	37.0	33.0	37.0
5	35.5377954397512	37.0	35.0	37.0	33.0	37.0
6	35.59788119371291	37.0	35.0	37.0	33.0	37.0
7	35.963706919983665	37.0	35.0	37.0	35.0	37.0
8	35.94982936795621	37.0	35.0	37.0	35.0	37.0
9	37.683493287595695	39.0	37.0	39.0	35.0	39.0
10	37.09252948691958	39.0	37.0	39.0	33.0	39.0
11	36.85522203137458	39.0	37.0	39.0	32.0	39.0
12	36.29175673903023	38.0	35.0	39.0	32.0	39.0
13	36.100184181449364	38.0	35.0	39.0	32.0	39.0
14	37.11696447259504	39.0	35.0	41.0	32.0	41.0
15	37.321065398601306	39.0	35.0	41.0	32.0	41.0
16	37.3951294513087	39.0	35.0	41.0	32.0	41.0
17	37.32824473310549	39.0	35.0	41.0	32.0	41.0
18	37.236915982582744	39.0	35.0	41.0	32.0	41.0
19	37.18252385743969	39.0	35.0	41.0	32.0	41.0
20	37.05017885626569	39.0	35.0	41.0	32.0	41.0
21	36.936710911609765	38.0	35.0	41.0	32.0	41.0
22	36.827854288170975	38.0	35.0	41.0	31.0	41.0
23	36.742767722067356	38.0	35.0	41.0	31.0	41.0
24	36.63460974627864	38.0	35.0	41.0	31.0	41.0
25	36.54100699840162	38.0	35.0	40.0	31.0	41.0
26	36.40492120989818	38.0	35.0	40.0	31.0	41.0
27	36.29949203093459	38.0	35.0	40.0	31.0	41.0
28	36.24642811762776	38.0	35.0	40.0	30.0	41.0
29	36.27121510000037	38.0	35.0	40.0	30.0	41.0
30	36.24577470319811	38.0	35.0	40.0	30.0	41.0
31	36.15656944676893	38.0	35.0	40.0	30.0	41.0
32	36.06246584377844	38.0	35.0	40.0	30.0	41.0
33	35.93945903535634	38.0	35.0	40.0	30.0	41.0
34	35.802937363092404	38.0	35.0	40.0	29.0	41.0
35	35.69118992565715	38.0	35.0	40.0	29.0	41.0
36	35.547782523610714	38.0	35.0	40.0	28.0	41.0
37	35.502060784401614	38.0	34.0	40.0	28.0	41.0
38	35.4026402219553	38.0	34.0	40.0	28.0	41.0
39	35.34271113942071	38.0	34.0	40.0	27.0	41.0
40	35.193244130681244	37.0	34.0	40.0	27.0	41.0
41	35.1438371176405	37.0	34.0	40.0	27.0	41.0
42	35.08549736598733	37.0	34.0	40.0	27.0	41.0
43	34.98344381890099	37.0	34.0	40.0	26.0	41.0
44	34.85799194930919	37.0	34.0	40.0	26.0	41.0
45	34.73154577129014	37.0	34.0	40.0	26.0	41.0
46	34.69563758485855	36.0	34.0	40.0	26.0	41.0
47	34.646986789021156	36.0	34.0	40.0	26.0	41.0
48	34.53758489967066	36.0	34.0	40.0	25.0	41.0
49	34.476123233385735	36.0	34.0	40.0	25.0	41.0
50	34.36451108440067	36.0	34.0	40.0	24.0	41.0
51	33.97584998361324	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	4.0
11	1.0
12	14.0
13	20.0
14	34.0
15	78.0
16	181.0
17	472.0
18	1041.0
19	2005.0
20	3524.0
21	5735.0
22	8829.0
23	12611.0
24	18503.0
25	26451.0
26	34533.0
27	40685.0
28	44592.0
29	50408.0
30	59921.0
31	72750.0
32	90372.0
33	116216.0
34	194807.0
35	311071.0
36	182182.0
37	227292.0
38	336329.0
39	590796.0
40	379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.98682685257795	23.39593748110999	28.407284851271115	13.20995081504095
2	31.4401311598908	26.128723053850972	28.863688045394415	13.567457740863814
3	28.85875351225676	25.414778351051737	31.37980649228301	14.346661644408496
4	25.560305957502976	28.225611789586573	30.49973250718283	15.714349745727619
5	23.564040576665988	31.978529846318075	29.601112901706976	14.856316675308953
6	20.963459370904594	41.29825922007237	27.009537218921796	10.728744190101244
7	86.92858620279861	4.174985124438646	6.904481008421191	1.991947664341542
8	87.37931468381362	3.502737226652565	6.486443809443133	2.6315042800906805
9	81.90424456204168	5.2600889614082496	9.229139569568899	3.6065269069811716
10	44.13927555296584	24.042402443251838	17.029814038006595	14.788507965775723
11	38.067949343727655	24.926835265956942	22.277237697694872	14.727977692620527
12	34.001277221660466	22.990688947180345	25.692798172248928	17.315235658910268
13	23.112325189023462	33.21450703397138	27.046505096344703	16.626662680660452
14	19.261826739494893	34.963346699064616	28.736623817099883	17.038202744340605
15	18.364358525084494	24.104289713019888	40.88515655423196	16.64619520766366
16	20.34561470096112	21.495772133128767	39.087752858842336	19.070860307067772
17	20.373206965422494	22.531571759831337	28.430394914799116	28.664826359947053
18	23.547386527326417	23.63045116847689	32.487938150561654	20.334224153635045
19	28.107018509845012	26.106147564746212	25.905805519357557	19.881028406051218
20	29.97469818133669	24.433011862206452	25.546777112483916	20.04551284397294
21	24.40817471208027	27.963217989991946	27.523057634113414	20.105549663814372
22	24.20787378780109	24.15667800649796	26.71737173606334	24.91807646963761
23	21.788143221534632	28.933306083744785	25.787911298477162	23.490639396243424
24	21.509547704804714	25.299351396740168	34.196232401707185	18.994868496747937
25	21.016957934338635	25.75999006513995	31.64088441637426	21.582167584147154
26	19.295463807049885	31.195707285139118	27.67598704027114	21.83284186753986
27	19.9041795906887	30.75838428581474	28.125152919125878	21.21228320437068
28	18.85879874547719	28.84131816183706	32.9477955178813	19.35208757480444
29	19.494942309139454	25.98459356512206	32.41355006351155	22.106914062226927
30	21.993090831185096	28.570083323704143	29.973258942504877	19.463566902605887
31	26.75717697004039	27.73487246904711	25.75164247991542	19.756308080997073
32	27.549621870837772	26.859321805989783	26.608647522597078	18.982408800575367
33	26.265944196187167	28.02132211768779	24.872267553676412	20.840466132448626
34	21.227127924893114	28.70541289500424	28.368055312826783	21.699403867275862
35	21.38869276404173	27.943315373003415	29.7750551948092	20.892936668145655
36	26.465833909371543	28.221581920857492	26.514644666324816	18.797939503446155
37	22.4442305232949	30.58559338377797	27.92012306725645	19.050053025670675
38	21.805043997531087	31.638951724228683	26.07061892615512	20.48538535208511
39	22.46117242040084	29.90109139536672	26.348762110680756	21.288974073551685
40	24.732373539223985	26.574311396180917	26.70898302972933	21.984332034865766
41	20.064839765428744	25.516429733687357	28.22310340190827	26.19562709897563
42	23.239759507303315	26.415460550258015	25.869372216357895	24.475407726080775
43	22.55192670902415	26.343827577543106	27.405739108765747	23.698506604667
44	21.27630877183171	29.14964424072133	27.828834204209898	21.74521278323706
45	19.363313637692595	32.048147884668445	25.881914154749424	22.70662432288953
46	22.431976432669735	30.354369385169505	26.314508226483557	20.899145955677202
47	22.27880029985513	27.67791973241672	27.828628598662497	22.214651369065656
48	22.933324999455145	26.109026042409845	29.60395025826113	21.35369869987388
49	22.324691458035293	25.80489431669258	29.548190033805668	22.322224191466464
50	21.292921700061804	29.905738080738008	27.996937299765897	20.80440291943429
51	20.823688719780613	30.6571852353834	25.82746980579734	22.691656239038654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2830.0
1	3273.5
2	3717.0
3	12056.5
4	20396.0
5	14632.0
6	8868.0
7	9267.0
8	9666.0
9	10490.0
10	11314.0
11	11570.5
12	11827.0
13	12038.0
14	12249.0
15	11719.0
16	11189.0
17	11018.5
18	10848.0
19	10777.0
20	10706.0
21	10861.0
22	11016.0
23	11603.0
24	12190.0
25	12368.5
26	15766.0
27	18985.0
28	21543.0
29	24101.0
30	29879.5
31	35658.0
32	43733.5
33	51809.0
34	56182.5
35	60556.0
36	67571.0
37	74586.0
38	84451.5
39	94317.0
40	117990.0
41	141663.0
42	170670.0
43	199677.0
44	214386.0
45	229095.0
46	228101.5
47	227108.0
48	213792.0
49	200476.0
50	187747.0
51	175018.0
52	164543.0
53	154068.0
54	140851.0
55	127634.0
56	114057.0
57	100480.0
58	93601.0
59	86722.0
60	81173.5
61	75625.0
62	66405.0
63	57185.0
64	51625.0
65	46065.0
66	39343.0
67	32621.0
68	28320.5
69	24020.0
70	20012.0
71	16004.0
72	13476.5
73	10949.0
74	9369.5
75	6503.0
76	5216.0
77	3967.0
78	2718.0
79	1923.5
80	1129.0
81	874.5
82	620.0
83	441.5
84	263.0
85	215.5
86	168.0
87	127.5
88	87.0
89	57.5
90	28.0
91	18.5
92	9.0
93	9.5
94	10.0
95	12.5
96	15.0
97	8.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2431841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.905293099770166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00513401506493	16.725307760713243
2	7.175816391438616	3.0002508978631877
3	2.436324035233396	1.527962041277068
4	1.306511196867422	1.092519980345799
5	0.8556729131671407	0.8944046523646634
6	0.6105313898063717	0.7658002590307339
7	0.45150034586615617	0.660712294548572
8	0.394881718572761	0.660409445320362
9	0.3258977250552923	0.6131688716546277
>10	3.971462731323847	19.732455308977894
>50	1.4784502795499241	22.187366797403943
>100	0.9836332816935708	30.739624192275205
>500	0.0025900806042353066	0.3776502738941687
>1k	0.0013946587868959345	0.5330920762734603
>5k	0.0	0.0
>10k+	1.9923696955656206E-4	0.48927514805721845
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11747	0.48304967306661906	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4863	0.19997195540333434	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.112110948043067E-5	0.0	0.0	0.05218268793066652	0.0
2	4.112110948043067E-5	0.0	0.0	0.17131054209547417	0.0
3	4.112110948043067E-5	0.0	0.0	0.27448340578187475	0.0
4	4.112110948043067E-5	0.0	0.0	0.47515442004637637	0.0
5	4.112110948043067E-5	0.0	0.0	0.7921570530310165	0.0
6	4.112110948043067E-5	0.0	0.0	1.435373447523913	0.0
7	4.112110948043067E-5	0.0	0.0	1.732144494644181	0.0
8	4.112110948043067E-5	0.0	0.0	2.54074999146737	0.0
9	4.112110948043067E-5	0.0	0.0	2.935389279151063	0.0
10	4.112110948043067E-5	0.0	0.0	3.5342359965145747	0.0
11	4.112110948043067E-5	0.0	0.0	3.933357485131635	0.0
12	4.112110948043067E-5	0.0	0.0	4.274251482724405	0.0
13	4.112110948043067E-5	0.0	0.0	4.454649790015054	0.0
14	8.224221896086134E-5	0.0	0.0	4.535041559049296	0.0
15	8.224221896086134E-5	0.0	0.0	4.618311805747169	0.0
16	8.224221896086134E-5	0.0	0.0	4.777532741655396	0.0
17	8.224221896086134E-5	0.0	0.0	4.948185345999184	0.0
18	8.224221896086134E-5	0.0	0.0	5.2204482118691145	0.0
19	8.224221896086134E-5	0.0	0.0	5.3351761073195165	0.0
20	8.224221896086134E-5	0.0	0.0	5.468819713130916	0.0
21	8.224221896086134E-5	0.0	0.0	5.635031237650817	0.0
22	8.224221896086134E-5	0.0	0.0	5.799968007776824	0.0
23	8.224221896086134E-5	0.0	0.0	5.993771796758094	0.0
24	8.224221896086134E-5	0.0	0.0	6.146289991821011	0.0
25	8.224221896086134E-5	0.0	0.0	6.268666413634773	0.0
26	8.224221896086134E-5	0.0	0.0	6.396511943009432	0.0
27	8.224221896086134E-5	0.0	0.0	6.530649002134597	0.0
28	8.224221896086134E-5	0.0	0.0	6.663141216880544	0.0
29	8.224221896086134E-5	0.0	0.0	6.812945418717753	0.0
30	8.224221896086134E-5	0.0	0.0	7.032203174467409	0.0
31	8.224221896086134E-5	0.0	0.0	7.190272719310185	0.0
32	8.224221896086134E-5	0.0	0.0	7.360349628121247	0.0
33	8.224221896086134E-5	0.0	0.0	7.517226660789089	0.0
34	8.224221896086134E-5	0.0	0.0	7.681916704258215	0.0
35	8.224221896086134E-5	0.0	0.0	7.8672084235770345	0.0
36	8.224221896086134E-5	0.0	0.0	8.026799449470586	0.0
37	8.224221896086134E-5	0.0	0.0	8.191736219596594	0.0
38	8.224221896086134E-5	0.0	0.0	8.37480739900347	0.0
39	8.224221896086134E-5	0.0	0.0	8.599328656766623	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	40	6.8212103E-9	45.000004	35
TCTAGCG	50	2.1827873E-11	45.000004	1
TCGTATA	20	7.0348405E-4	45.000004	28
TATCGTT	25	3.8920938E-5	45.000004	19
AGTACGA	55	1.8189894E-12	45.0	34
GTACGAC	50	1.0822987E-9	40.500004	35
AACACGT	1245	0.0	40.301205	41
TACGGGA	565	0.0	40.22124	4
CACGACC	1365	0.0	40.21978	27
ACACGAC	1365	0.0	40.054947	26
CGTTTTT	8440	0.0	39.96149	1
CACGTGA	1265	0.0	39.486168	43
ACGACCA	1395	0.0	39.35484	28
AGGGTAC	1285	0.0	39.22179	6
TAGGGCG	1280	0.0	39.02344	5
CGAGACA	1400	0.0	38.892857	22
GACACGA	1415	0.0	38.798588	25
GCGAGAC	1400	0.0	38.732143	21
AGGGCGA	3125	0.0	38.664	6
AGACACG	1465	0.0	38.24232	24
>>END_MODULE