FastQCFastQC Report
Thu 26 May 2016
SRR1546668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546668_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1039463
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203501.9577416415976328No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT11270.1084213675715249No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10870.10457322675265979No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACG207.0329756E-445.01
CTAAACG207.0329756E-445.045
TATAACG253.890548E-545.01
CGTTTTT117200.043.8097271
ACGGGTA700.041.7857135
CGTTCGG1650.040.9090945
TATACGG1000.040.52
ATAACGG950.040.2631572
AATCGAG451.9279469E-840.01
TACGGGA3050.039.8360674
CGTAAGG1600.039.3752
TAGGGAT7450.038.9597325
CTCACGA356.247714E-638.57142624
CGACGGT356.247714E-638.57142628
GTTTTTT136250.038.4110072
ACGTAGG1000.038.252
AGGGCGA11650.038.047216
TAAGGGA9650.037.538864
CTATACG301.1398469E-437.4999961
ATAGGGC5250.037.2857174