##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546668_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1039463
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.261642790556277	31.0	31.0	33.0	30.0	34.0
2	31.64807693972753	31.0	31.0	34.0	30.0	34.0
3	31.565801764949786	31.0	31.0	34.0	30.0	34.0
4	35.46918360730493	37.0	35.0	37.0	33.0	37.0
5	35.42488765833897	37.0	35.0	37.0	33.0	37.0
6	35.50092788295495	37.0	35.0	37.0	33.0	37.0
7	35.92954150364179	37.0	35.0	37.0	35.0	37.0
8	35.91709950233919	37.0	35.0	37.0	35.0	37.0
9	37.63287582145781	39.0	37.0	39.0	35.0	39.0
10	37.04912247958802	39.0	37.0	39.0	33.0	39.0
11	36.81834851264547	39.0	37.0	39.0	32.0	39.0
12	36.41254089852164	39.0	35.0	39.0	32.0	39.0
13	36.31292984935491	39.0	35.0	39.0	32.0	39.0
14	37.40205567682544	40.0	35.0	41.0	32.0	41.0
15	37.55670957023001	40.0	35.0	41.0	32.0	41.0
16	37.58176673917205	40.0	35.0	41.0	32.0	41.0
17	37.50361965745775	39.0	35.0	41.0	32.0	41.0
18	37.27426757854777	39.0	36.0	41.0	32.0	41.0
19	37.085948225189355	38.0	35.0	41.0	32.0	41.0
20	36.81732298311724	38.0	35.0	41.0	32.0	41.0
21	36.71880191983745	38.0	35.0	40.0	31.0	41.0
22	36.62795308731528	38.0	35.0	40.0	31.0	41.0
23	36.52558773135744	38.0	35.0	40.0	31.0	41.0
24	36.36831902626645	38.0	35.0	40.0	31.0	41.0
25	36.24794340924112	38.0	35.0	40.0	31.0	41.0
26	36.09116534210453	37.0	35.0	40.0	30.0	41.0
27	35.987270350171194	37.0	35.0	40.0	30.0	41.0
28	35.88294821460696	37.0	35.0	40.0	30.0	41.0
29	35.85636525783025	37.0	35.0	40.0	30.0	41.0
30	35.76400506800146	37.0	35.0	40.0	30.0	41.0
31	35.59820503471504	37.0	35.0	40.0	29.0	41.0
32	35.269980749675554	37.0	34.0	40.0	27.0	41.0
33	34.882323853759104	37.0	34.0	40.0	24.0	41.0
34	34.476041956279346	37.0	34.0	40.0	22.0	41.0
35	34.14280546782329	37.0	33.0	40.0	21.0	41.0
36	33.94723429309172	37.0	33.0	40.0	18.0	41.0
37	33.85484235610118	37.0	33.0	40.0	18.0	41.0
38	33.76402815684637	37.0	33.0	40.0	18.0	41.0
39	33.642632782504045	37.0	33.0	40.0	18.0	41.0
40	33.53290400908931	36.0	33.0	40.0	18.0	41.0
41	33.46551151892852	36.0	33.0	40.0	17.0	41.0
42	33.36908192018379	36.0	33.0	40.0	16.0	41.0
43	33.233676427155174	36.0	33.0	40.0	15.0	41.0
44	33.09239386106095	36.0	33.0	40.0	15.0	41.0
45	32.99553615665012	35.0	32.0	40.0	15.0	41.0
46	32.950687999476656	35.0	32.0	40.0	15.0	41.0
47	32.90775717846619	35.0	32.0	40.0	15.0	41.0
48	32.79691052014358	35.0	32.0	40.0	15.0	41.0
49	32.7197283597396	35.0	32.0	40.0	15.0	41.0
50	32.61955836811892	35.0	32.0	40.0	15.0	41.0
51	32.25929638669197	35.0	31.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	2.0
14	11.0
15	35.0
16	108.0
17	257.0
18	646.0
19	1300.0
20	2199.0
21	3559.0
22	5479.0
23	8129.0
24	12621.0
25	20133.0
26	28203.0
27	30380.0
28	28154.0
29	26912.0
30	28748.0
31	32127.0
32	38751.0
33	49017.0
34	74557.0
35	115016.0
36	84956.0
37	98401.0
38	133239.0
39	216401.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.289004995848817	22.994950277210442	25.736654407131375	20.97939031980936
2	37.47175224130152	23.630470733446018	26.3879522407243	12.509824784528165
3	26.427299480597195	23.518874649698933	37.0984825818716	12.955343287832275
4	22.510854162197212	26.22075052214461	37.249906923093945	14.018488392564239
5	20.41900481306213	30.03743278981551	36.04062867076558	13.502933726356783
6	18.66040445884077	38.2340689375187	33.13643679476807	9.969089808872464
7	79.67902657429846	3.639860197044051	14.89855819783869	1.7825550308187978
8	79.85767651181426	3.091788740917185	14.601096912540418	2.449437834728124
9	74.38898739060458	5.253962863517027	16.979344142119537	3.377705603758864
10	38.492760204066904	25.925405714296705	23.041512781118712	12.54032130051767
11	31.27114673634367	23.45326384873728	30.168654391738812	15.106935023180238
12	27.15248161791233	21.773646584823126	33.908277639511944	17.165594157752608
13	20.80968730969741	27.959725358189758	35.23376974457003	15.9968175875428
14	17.557912114235908	31.34782094215956	34.72071636989484	16.373550573709693
15	17.03264089246082	23.944960041867773	43.643015672515524	15.37938339315589
16	18.79008680443652	22.321910447990934	41.908658605453006	16.979344142119537
17	18.890138465727016	22.62437431635373	34.62441664590274	23.861070572016512
18	20.5360844974761	23.85260466221501	37.14870081955779	18.4626100207511
19	24.261373420698956	25.58696172927752	32.06732707176687	18.084337778256657
20	25.184927217226587	24.745854349794076	31.098172806535686	18.971045626443654
21	21.848108109668164	27.258594100992532	32.90458631043144	17.98871147890786
22	21.60327015006787	24.316882852011087	32.12629982981598	21.953547168105068
23	19.550287023203328	27.635038476598012	31.50395925588501	21.31071524431365
24	19.80763144046493	24.901607849437642	37.587966094031245	17.702794616066182
25	19.846786273296885	25.85027076480837	35.33699612203609	18.965946839858656
26	18.050474139050646	29.729196710224414	32.658497705065024	19.561831445659923
27	18.80894269444896	28.488363703181353	33.21994145053744	19.482752151832244
28	17.92098420049583	27.51016630702584	37.000547398031486	17.568302094446846
29	18.910245001505587	25.04100675060103	36.412455277388425	19.63629297050496
30	20.40851862933072	27.08706322399162	34.2112225254771	18.29319562120056
31	23.535229248179107	26.51927004616807	30.829572577378894	19.115928128273925
32	24.745758146273605	26.958150506559637	31.179657188375153	17.11643415879161
33	23.032084836112492	27.453983451070407	30.091980185922925	19.421951526894173
34	20.56581138530183	28.81786076079668	30.669970936916464	19.94635691698502
35	19.876801771684033	28.065549230708548	32.03808120154349	20.019567796063928
36	24.001239101343675	28.05544786105903	29.78990113164201	18.153411905955284
37	20.42391119260618	30.622927415405837	30.81389140354202	18.139269988445957
38	20.801702417498266	31.055169832884864	28.801890976398393	19.34123677321848
39	20.135300631191296	30.50729078379894	29.485898006951665	19.871510578058093
40	22.47593228426601	27.993877607957184	29.184011359711697	20.346178748065107
41	19.14854112171381	27.331420165989556	29.71986496873867	23.800173743557973
42	21.429237981534698	28.355218030848622	27.84101021392777	22.374533773688913
43	21.1345665983301	27.915086924690925	29.7224624637914	21.227884013187577
44	21.034322529998665	30.101215723888195	28.523574191673973	20.340887554439167
45	19.220597558547055	31.7467769415554	27.778189315059798	21.254436184837747
46	21.862153823657025	29.847430836884044	27.928363010516005	20.362052328942926
47	20.961881279083528	28.996991715914856	28.728295283237593	21.312831721764024
48	21.329667337846562	27.66139824120724	29.51216156804042	21.496772852905778
49	20.839221790482203	26.8378961059701	30.385400923361388	21.937481180186307
50	20.007061338402618	29.06818232106386	30.02088578429439	20.903870556239134
51	19.361728123078937	30.662274655278736	28.382347423621617	21.59364979802071
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1079.0
1	1289.5
2	1500.0
3	15206.0
4	28912.0
5	19752.5
6	10593.0
7	11038.0
8	11483.0
9	12552.0
10	13621.0
11	14597.5
12	15574.0
13	15759.5
14	15945.0
15	15343.0
16	14741.0
17	13863.5
18	12986.0
19	11907.5
20	10829.0
21	10409.5
22	9990.0
23	9419.5
24	8849.0
25	9042.5
26	9745.0
27	10254.0
28	10975.5
29	11697.0
30	13173.5
31	14650.0
32	16323.5
33	17997.0
34	20784.0
35	23571.0
36	26200.0
37	28829.0
38	32093.0
39	35357.0
40	44104.0
41	52851.0
42	61945.0
43	71039.0
44	78116.0
45	85193.0
46	86503.5
47	87814.0
48	83971.5
49	80129.0
50	72994.5
51	65860.0
52	60897.5
53	55935.0
54	51540.5
55	47146.0
56	44197.5
57	41249.0
58	37948.0
59	34647.0
60	31921.0
61	29195.0
62	26395.0
63	23595.0
64	20816.5
65	18038.0
66	15528.0
67	13018.0
68	11198.5
69	9379.0
70	8011.0
71	6643.0
72	5185.5
73	3728.0
74	3247.5
75	2301.5
76	1836.0
77	1305.0
78	774.0
79	655.5
80	537.0
81	379.0
82	221.0
83	164.5
84	108.0
85	68.5
86	29.0
87	24.5
88	20.0
89	13.5
90	7.0
91	6.5
92	6.0
93	4.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1039463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.73083771588286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75299881895647	28.192019438264392
2	7.492575996034223	5.504171859685049
3	3.3652152217619613	3.7082152256867214
4	2.053201894673762	3.0166330236482066
5	1.4054717142522828	2.5812076725232145
6	1.073867140752507	2.3666423805239685
7	0.8409062779007999	2.1621034419488967
8	0.6961242217101979	2.045538065418604
9	0.5940401357859979	1.9637632641848857
>10	5.539801145648244	40.92008912682326
>50	0.1642181094961942	3.748541415028845
>100	0.019210860745898256	1.1974461547057442
>500	0.0015789748543188376	0.4125524850883455
>1k	5.263249514396125E-4	0.21400918509503208
>5k	0.0	0.0
>10k+	2.6316247571980627E-4	1.967067261374843
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20350	1.9577416415976328	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	1127	0.1084213675715249	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1087	0.10457322675265979	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.620352047162814E-5	0.0	0.0	0.0605120143766541	0.0
2	9.620352047162814E-5	0.0	0.0	0.19221463390231303	0.0
3	9.620352047162814E-5	0.0	0.0	0.3471023018616343	0.0
4	9.620352047162814E-5	0.0	0.0	0.530851025962444	0.0
5	9.620352047162814E-5	0.0	0.0	0.9187436205040487	0.0
6	9.620352047162814E-5	0.0	0.0	1.749364816256086	0.0
7	9.620352047162814E-5	0.0	0.0	2.2254760390701738	0.0
8	9.620352047162814E-5	0.0	0.0	3.2886211438021364	0.0
9	9.620352047162814E-5	0.0	0.0	3.8427534217187143	0.0
10	9.620352047162814E-5	0.0	0.0	4.530993407172742	0.0
11	9.620352047162814E-5	0.0	0.0	4.915134064415954	0.0
12	9.620352047162814E-5	0.0	0.0	5.299659535741051	0.0
13	9.620352047162814E-5	0.0	0.0	5.506304697714108	0.0
14	9.620352047162814E-5	0.0	0.0	5.61867040962497	0.0
15	9.620352047162814E-5	0.0	0.0	5.714392912494239	0.0
16	9.620352047162814E-5	0.0	0.0	5.876014826886575	0.0
17	9.620352047162814E-5	0.0	0.0	6.046679872203243	0.0
18	9.620352047162814E-5	0.0	0.0	6.332789142085866	0.0
19	9.620352047162814E-5	0.0	0.0	6.4463092962423865	0.0
20	9.620352047162814E-5	0.0	0.0	6.5808018178617225	0.0
21	9.620352047162814E-5	0.0	0.0	6.722509603516431	0.0
22	9.620352047162814E-5	0.0	0.0	6.884516331990653	0.0
23	9.620352047162814E-5	0.0	0.0	7.065378950477314	0.0
24	9.620352047162814E-5	0.0	0.0	7.216995698740599	0.0
25	9.620352047162814E-5	0.0	0.0	7.349083132348145	0.0
26	9.620352047162814E-5	0.0	0.0	7.476745204013996	0.0
27	9.620352047162814E-5	0.0	0.0	7.615951698136442	0.0
28	9.620352047162814E-5	0.0	0.0	7.749866998632948	0.0
29	9.620352047162814E-5	0.0	0.0	7.908025586288304	0.0
30	9.620352047162814E-5	0.0	0.0	8.133430434753329	0.0
31	9.620352047162814E-5	0.0	0.0	8.290145969601612	0.0
32	9.620352047162814E-5	0.0	0.0	8.458886944508848	0.0
33	9.620352047162814E-5	0.0	0.0	8.632053281357779	0.0
34	9.620352047162814E-5	0.0	0.0	8.808394334382273	0.0
35	9.620352047162814E-5	0.0	0.0	8.991084819757894	0.0
36	9.620352047162814E-5	0.0	0.0	9.16992716431465	0.0
37	9.620352047162814E-5	0.0	0.0	9.34174665187698	0.0
38	9.620352047162814E-5	0.0	0.0	9.530016941439955	0.0
39	9.620352047162814E-5	0.0	0.0	9.746955880103476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGACG	20	7.0329756E-4	45.0	1
CTAAACG	20	7.0329756E-4	45.0	45
TATAACG	25	3.890548E-5	45.0	1
CGTTTTT	11720	0.0	43.809727	1
ACGGGTA	70	0.0	41.785713	5
CGTTCGG	165	0.0	40.90909	45
TATACGG	100	0.0	40.5	2
ATAACGG	95	0.0	40.263157	2
AATCGAG	45	1.9279469E-8	40.0	1
TACGGGA	305	0.0	39.836067	4
CGTAAGG	160	0.0	39.375	2
TAGGGAT	745	0.0	38.959732	5
CTCACGA	35	6.247714E-6	38.571426	24
CGACGGT	35	6.247714E-6	38.571426	28
GTTTTTT	13625	0.0	38.411007	2
ACGTAGG	100	0.0	38.25	2
AGGGCGA	1165	0.0	38.04721	6
TAAGGGA	965	0.0	37.53886	4
CTATACG	30	1.1398469E-4	37.499996	1
ATAGGGC	525	0.0	37.285717	4
>>END_MODULE