FastQCFastQC Report
Thu 26 May 2016
SRR1546667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546667_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences695727
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34590.4971777723158653No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24790.3563179235533478No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8150.11714364973617526No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA207.031364E-445.00000413
AACGTAG302.1642609E-645.0000041
TAATACG406.8084773E-945.0000041
CGTATGA207.031364E-445.00000427
ACGGGTA406.8084773E-945.0000045
TCTAGCG207.031364E-445.0000041
TAGCCGT207.031364E-445.00000444
ACGGGCC302.1642609E-645.0000045
ATGTACG302.1642609E-645.0000041
ACGATAT207.031364E-445.00000445
CGCTCTA207.031364E-445.00000412
CTACGAT207.031364E-445.00000443
TGTTACG207.031364E-445.0000041
TTACGAG207.031364E-445.0000041
ATCGGTA207.031364E-445.00000427
TCGAAGT302.1642609E-645.00000440
ATACGAG453.8380676E-1045.01
TACGTGG453.8380676E-1045.02
GTATGCG650.041.538461
AATACGG650.041.538462