##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546667_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	695727
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35484176983213	31.0	31.0	33.0	30.0	34.0
2	31.76767323964716	31.0	31.0	34.0	30.0	34.0
3	31.878110235767764	31.0	31.0	34.0	30.0	34.0
4	35.641408196030916	37.0	35.0	37.0	33.0	37.0
5	35.55092442869114	37.0	35.0	37.0	33.0	37.0
6	35.61598011863849	37.0	35.0	37.0	33.0	37.0
7	35.95488747741571	37.0	35.0	37.0	35.0	37.0
8	35.93668781001743	37.0	35.0	37.0	35.0	37.0
9	37.66011955839	39.0	37.0	39.0	35.0	39.0
10	37.151034816817514	39.0	37.0	39.0	33.0	39.0
11	36.86276944836121	39.0	37.0	39.0	33.0	39.0
12	36.080104696238614	37.0	35.0	39.0	32.0	39.0
13	35.78235141082637	37.0	35.0	39.0	31.0	39.0
14	36.788059109392044	39.0	35.0	41.0	31.0	41.0
15	37.06949708721955	39.0	35.0	41.0	32.0	41.0
16	37.18685633876506	39.0	35.0	41.0	32.0	41.0
17	37.12172446951175	38.0	35.0	41.0	32.0	41.0
18	37.02981198084881	38.0	35.0	41.0	32.0	41.0
19	36.942820962820186	38.0	35.0	41.0	32.0	41.0
20	36.77205858044894	38.0	35.0	41.0	32.0	41.0
21	36.65530732600575	38.0	35.0	41.0	32.0	41.0
22	36.51674722987609	38.0	35.0	40.0	31.0	41.0
23	36.45619474305295	38.0	35.0	40.0	31.0	41.0
24	36.320608514546656	38.0	35.0	40.0	31.0	41.0
25	36.2043574563011	37.0	35.0	40.0	31.0	41.0
26	36.018688364832755	37.0	35.0	40.0	31.0	41.0
27	35.94335421796193	37.0	35.0	40.0	30.0	41.0
28	35.903049615725706	37.0	35.0	40.0	30.0	41.0
29	35.95424498402391	37.0	35.0	40.0	30.0	41.0
30	35.9278768827428	37.0	35.0	40.0	30.0	41.0
31	35.80905153889385	37.0	35.0	40.0	30.0	41.0
32	35.64980804252242	37.0	34.0	40.0	30.0	41.0
33	35.5070825194365	37.0	34.0	40.0	29.0	41.0
34	35.392712946313715	37.0	34.0	40.0	29.0	41.0
35	35.26162129685925	37.0	34.0	40.0	28.0	41.0
36	35.09273465022918	37.0	34.0	40.0	27.0	41.0
37	35.0002256632271	36.0	34.0	40.0	27.0	41.0
38	34.95063868442651	36.0	34.0	40.0	27.0	41.0
39	34.8779046953762	36.0	34.0	40.0	27.0	41.0
40	34.707074757771366	36.0	34.0	40.0	25.0	41.0
41	34.65910191784996	36.0	34.0	40.0	25.0	41.0
42	34.58615232699033	36.0	34.0	40.0	25.0	41.0
43	34.45504199204573	35.0	34.0	40.0	25.0	41.0
44	34.30836808115827	35.0	34.0	40.0	24.0	41.0
45	34.200373134864684	35.0	33.0	40.0	23.0	41.0
46	34.16350378812379	35.0	33.0	40.0	23.0	41.0
47	34.10890909796515	35.0	33.0	40.0	23.0	41.0
48	34.04070705894697	35.0	33.0	40.0	23.0	41.0
49	33.97389062089009	35.0	33.0	40.0	24.0	41.0
50	33.86381296111837	35.0	33.0	39.0	24.0	41.0
51	33.479657969289676	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	4.0
14	17.0
15	22.0
16	55.0
17	146.0
18	341.0
19	628.0
20	1092.0
21	1789.0
22	2769.0
23	4175.0
24	5972.0
25	8195.0
26	10847.0
27	12620.0
28	13729.0
29	15158.0
30	17998.0
31	21800.0
32	27106.0
33	35247.0
34	62871.0
35	108412.0
36	50689.0
37	60623.0
38	85840.0
39	147467.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56895305198735	24.723490679533782	28.616684417882297	14.09087185059657
2	31.46464058459712	27.068807161429696	28.340139163781195	13.126413090191985
3	28.017742591562495	26.17262230731307	31.105735439331806	14.703899661792628
4	24.85155815427603	29.425622406489904	30.55652576369754	15.166293675536524
5	23.099290382578225	32.72562370010076	29.821323593880933	14.353762323440087
6	21.35507174509542	40.85165589376293	26.7423860221035	11.050886339038158
7	86.01534797413353	4.577082677544497	7.622098897987286	1.7854704503346859
8	86.51338815368672	3.905698643289681	6.748049162961908	2.832864040061691
9	80.99556291476398	5.724371772261247	9.845097286723096	3.4349680262516764
10	50.03658044031639	22.282878197913835	14.482692205419653	13.19784915635012
11	43.007817721606315	21.046329954134308	21.634779159066703	14.31107316519267
12	37.99220096388382	20.587241834800142	25.64094824550434	15.77960895581169
13	20.67477616938828	37.06295716566987	26.976960790654953	15.285305874286898
14	16.147856846147988	38.89729735945278	28.817194100559558	16.13765169383968
15	14.523225345573767	23.296925374464408	46.4988422182839	15.68100706167793
16	16.624049375689026	18.537012362607747	45.24605197153481	19.592886290168412
17	16.923448421579153	20.063904376285528	29.228706087301486	33.78394111483384
18	21.527984396178386	22.384211048299118	34.3269702052673	21.7608343502552
19	28.25734806899833	25.16978642484768	25.716121409690867	20.856744096463125
20	30.532378361052537	22.70186438071255	25.035538364904625	21.730218893330285
21	23.240581434959402	28.149834633412247	27.324942110914197	21.28464182071416
22	23.365343015291916	24.30450449673507	25.645404016230504	26.68474847174251
23	20.574305726240322	29.700442846116363	24.36458553426847	25.360665893374843
24	20.35281080078824	23.920302072508328	36.835425389556534	18.891461737146898
25	18.950967836522086	24.59916030281993	33.71178637597793	22.738085484680052
26	17.773925692117743	32.84822926233997	27.151167052593905	22.226677992948385
27	18.250549425277445	31.851286495996273	28.320591266401905	21.577572812324377
28	17.2376521250433	27.648488559449326	36.098354670725726	19.015504644781643
29	17.683947870357194	23.976071073855117	35.95835722920053	22.381623826587152
30	19.74050166228995	29.762392432663958	30.50909336564486	19.988012539401232
31	28.65434286724534	25.869342428854996	24.888210461862194	20.588104242037467
32	29.538597754579023	25.442450846380837	25.75018649556507	19.26876490347507
33	26.83753828728797	27.496704885680735	23.76104420268295	21.904712624348342
34	20.95620839783421	27.017493930809067	27.251493761202312	24.774803910154414
35	20.936516765915368	25.812998489349994	30.04583694466364	23.204647800071005
36	29.025609182912266	24.704086516694048	26.98112909230201	19.28917520809168
37	21.120784445623066	30.606257914383082	28.993700115131364	19.27925752486248
38	22.347989944331612	30.391518512290023	25.154406829115445	22.106084714262924
39	21.46459746423525	30.620343899259332	26.519597485795433	21.395461150709977
40	25.215781477504827	25.84217660087937	26.36853248472461	22.573509436891197
41	18.59163148763811	23.829318108970902	29.058955596088698	28.520094807302286
42	23.579794948305874	24.659816278511542	25.508568734575487	26.2518200386071
43	23.83592989779037	25.212188114015987	26.272661546842368	24.679220441351276
44	21.58418460114384	29.110125092169774	27.676085591043613	21.629604715642774
45	18.843023197317336	34.53423541130357	24.003236901830746	22.61950448954834
46	23.41234420972594	30.493138831754408	25.70390397382882	20.390612984690833
47	22.702583053410315	27.281678014508564	26.661176007255722	23.354562924825398
48	23.79439061585938	23.932806977449488	30.223924039170534	22.048878367520594
49	21.87668438913539	23.524600885117295	31.17918378904369	23.41953093670362
50	19.709023798127713	30.485664635697624	27.53407586596467	22.271235700209996
51	19.599354344448326	31.74463546764751	24.846096241772994	23.80991394613117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1108.0
1	1220.0
2	1332.0
3	3663.0
4	5994.0
5	4449.5
6	2905.0
7	3147.5
8	3390.0
9	3706.0
10	4022.0
11	4276.5
12	4531.0
13	4402.5
14	4274.0
15	4189.0
16	4104.0
17	3923.0
18	3742.0
19	3632.5
20	3523.0
21	3373.0
22	3223.0
23	3479.5
24	3736.0
25	3610.0
26	4332.0
27	5180.0
28	5201.0
29	5222.0
30	6511.0
31	7800.0
32	9051.5
33	10303.0
34	12036.5
35	13770.0
36	15089.5
37	16409.0
38	21054.0
39	25699.0
40	34573.0
41	43447.0
42	52582.5
43	61718.0
44	61687.5
45	61657.0
46	61811.5
47	61966.0
48	61068.5
49	60171.0
50	56458.0
51	52745.0
52	47541.5
53	42338.0
54	38815.0
55	35292.0
56	31886.0
57	28480.0
58	27456.0
59	26432.0
60	24531.5
61	22631.0
62	20431.0
63	18231.0
64	16282.0
65	14333.0
66	12471.5
67	10610.0
68	9211.0
69	7812.0
70	6459.0
71	5106.0
72	4294.5
73	3483.0
74	3024.0
75	1871.0
76	1177.0
77	1036.5
78	896.0
79	685.5
80	475.0
81	352.0
82	229.0
83	162.5
84	96.0
85	66.5
86	37.0
87	28.5
88	20.0
89	18.5
90	17.0
91	10.5
92	4.0
93	4.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.652862679341364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.15781419464973	20.83129489047477
2	6.448248105726689	3.4372854256851313
3	2.529631287028109	2.022657459675773
4	1.4909664343133284	1.5895409453304155
5	1.0471594898597247	1.3954899043300204
6	0.8231340147272442	1.3163326716732118
7	0.6709005302682325	1.2516993793315556
8	0.567656049195413	1.2103726982642233
9	0.4667680762582816	1.1196634895651087
>10	6.5755297263428725	41.421234680926695
>50	1.1217899881882563	19.877932413286945
>100	0.09877396122412255	3.549682448385695
>500	5.42714072660014E-4	0.11788880176994058
>1k	0.001085428145320028	0.8589247913004996
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3459	0.4971777723158653	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2479	0.3563179235533478	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	815	0.11714364973617526	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4373453955358927E-4	0.0	0.0	0.03190906778089682	0.0
2	1.4373453955358927E-4	0.0	0.0	0.13870383066921363	0.0
3	1.4373453955358927E-4	0.0	0.0	0.23399983039324332	0.0
4	1.4373453955358927E-4	0.0	0.0	0.47504265322461253	0.0
5	1.4373453955358927E-4	0.0	0.0	0.8020387307090281	0.0
6	1.4373453955358927E-4	0.0	0.0	1.5185554103836707	0.0
7	1.4373453955358927E-4	0.0	0.0	1.8471325678031756	0.0
8	1.4373453955358927E-4	0.0	0.0	2.748923068962395	0.0
9	1.4373453955358927E-4	0.0	0.0	3.25529985180969	0.0
10	1.4373453955358927E-4	0.0	0.0	4.03017275454309	0.0
11	1.4373453955358927E-4	0.0	0.0	4.603098629203696	0.0
12	1.4373453955358927E-4	0.0	0.0	5.080872238679827	0.0
13	1.4373453955358927E-4	0.0	0.0	5.333845028294144	0.0
14	1.4373453955358927E-4	0.0	0.0	5.429859700715942	0.0
15	1.4373453955358927E-4	0.0	0.0	5.519118849778721	0.0
16	1.4373453955358927E-4	0.0	0.0	5.699649431458028	0.0
17	1.4373453955358927E-4	0.0	0.0	5.88190482761198	0.0
18	1.4373453955358927E-4	0.0	0.0	6.160606099806389	0.0
19	1.4373453955358927E-4	0.0	0.0	6.274012651514171	0.0
20	2.8746907910717854E-4	0.0	0.0	6.414441296658028	0.0
21	2.8746907910717854E-4	0.0	0.0	6.580454689842424	0.0
22	2.8746907910717854E-4	0.0	0.0	6.742587250458873	0.0
23	2.8746907910717854E-4	0.0	0.0	6.929154682799432	0.0
24	2.8746907910717854E-4	0.0	0.0	7.076051382223199	0.0
25	2.8746907910717854E-4	0.0	0.0	7.198513209922858	0.0
26	2.8746907910717854E-4	0.0	0.0	7.31594432873814	0.0
27	2.8746907910717854E-4	0.0	0.0	7.438262421898244	0.0
28	2.8746907910717854E-4	0.0	0.0	7.552675115382901	0.0
29	2.8746907910717854E-4	0.0	0.0	7.686635706246847	0.0
30	2.8746907910717854E-4	0.0	0.0	7.88297708727705	0.0
31	2.8746907910717854E-4	0.0	0.0	8.023980670579121	0.0
32	2.8746907910717854E-4	0.0	0.0	8.153916694335566	0.0
33	2.8746907910717854E-4	0.0	0.0	8.283996452631564	0.0
34	2.8746907910717854E-4	0.0	0.0	8.421550406984348	0.0
35	2.8746907910717854E-4	0.0	0.0	8.59460679260687	0.0
36	2.8746907910717854E-4	0.0	0.0	8.737478924923138	0.0
37	2.8746907910717854E-4	0.0	0.0	8.887106580598424	0.0
38	2.8746907910717854E-4	0.0	0.0	9.040040130683444	0.0
39	2.8746907910717854E-4	0.0	0.0	9.19168006991248	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	20	7.031364E-4	45.000004	13
AACGTAG	30	2.1642609E-6	45.000004	1
TAATACG	40	6.8084773E-9	45.000004	1
CGTATGA	20	7.031364E-4	45.000004	27
ACGGGTA	40	6.8084773E-9	45.000004	5
TCTAGCG	20	7.031364E-4	45.000004	1
TAGCCGT	20	7.031364E-4	45.000004	44
ACGGGCC	30	2.1642609E-6	45.000004	5
ATGTACG	30	2.1642609E-6	45.000004	1
ACGATAT	20	7.031364E-4	45.000004	45
CGCTCTA	20	7.031364E-4	45.000004	12
CTACGAT	20	7.031364E-4	45.000004	43
TGTTACG	20	7.031364E-4	45.000004	1
TTACGAG	20	7.031364E-4	45.000004	1
ATCGGTA	20	7.031364E-4	45.000004	27
TCGAAGT	30	2.1642609E-6	45.000004	40
ATACGAG	45	3.8380676E-10	45.0	1
TACGTGG	45	3.8380676E-10	45.0	2
GTATGCG	65	0.0	41.53846	1
AATACGG	65	0.0	41.53846	2
>>END_MODULE