FastQCFastQC Report
Thu 26 May 2016
SRR1546666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546666_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1852516
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430472.323704626572726No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47730.25764959654869374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29720.16043046321867124No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT24770.13371004622902044No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC22330.12053876997553598No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC20360.10990458382005878TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT19760.1066657453970708TruSeq Adapter, Index 22 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCC207.034407E-445.039
TACCCGC207.034407E-445.025
TACGCCC207.034407E-445.037
TCGTCGT253.891733E-545.018
TCGTAAT207.034407E-445.040
TCGCGTG207.034407E-445.01
ATCGACG253.891733E-545.01
CGCTTAA207.034407E-445.020
CGTTTTT159200.043.657351
ATTCGGC2350.041.1702117
ACTACGG1100.040.909092
AACGCCG2100.040.71428713
CGTAAGG3050.040.5737722
GCGAGAC1450.040.3448321
CGACGGT950.040.26315728
AAGGGCG6950.040.1438875
AGTAACG451.929402E-840.01
GTTGATC10000.039.82499716
AACACGT1300.039.80769341
ACGTTAG850.039.7058831
Produced by FastQC (version 0.11.5)